INVESTIGATION OF THE RELATIONSHIP BETWEEN PARKINSON'S DISEASE AND TIRAP GENE POLYMORPHISM

Merve Kır Kayan; Nilüfer Şahin Calapoğlu

Makale detayları

INVESTIGATION OF THE RELATIONSHIP BETWEEN PARKINSON'S DISEASE AND TIRAP GENE POLYMORPHISM

Yazarlar:

Merve Kır Kayan | Nilüfer Şahin Calapoğlu

Yayıncı:

Süleyman Demirel University

Ana Başlıklar:

Genomics

Dergi:

Medical Journal of Süleyman Demirel University Vol.31 Issue 1, pp.103-109

Belge Tipi:

Makale

Yayın Tarihi:

3/2024

Belge Dili:

Türkçe

Document ID (Org No.):

2001313405

pISSN:

1300-7416

Anahtar kelimeler:

Özet:

 Objective Parkinsons disease PD is a progressive disease characterized by the loss of dopamine-producing cells in the brain. A protein called α-synuclein accumulates in the brain tissue of patients with PD and causes neuroinflammation. Neuroinflammation involves the activation of immune system cells in the brain and the release of inflammation-related molecules. The Toll Like Receptors TLRs involved in this process recognize pathogens and damaged cells and initiate the immune response. In this study, we aimed to investigate the association of the polymorphic allele on the TollIL-1 receptor-associated protein TIRAP gene region, an adaptor protein involved in the TLR signaling pathway, in patients with PD and in the control group. Material and Method 39 PD patients and 40 healthy individuals participated in the study. Blood samples were taken from the participants and DNA isolation was performed. TIRAP rs8177374 975CT polymorphism was determined by PCR and RFLP methods. Results T allele frequency was found to be 0.218 in PD patients and 0.200 in the control group. C allele frequency and CC genotype frequency were found to be high in both groups. F value was found to be 0,128 in PD patients and 0,250 in the control group. OR value was 1.115; CI value was 0,517-2,402. Total OR1,508; P0,758. Conclusion No statistically significant association was found between TIRAP polymorphism and PD. It was concluded that TIRAP polymorphism is not a risk factor in PD patients.

 Objective Parkinsons disease PD is a progressive disease characterized by the loss of dopamine-producing cells in the brain. A protein called α-synuclein accumulates in the brain tissue of patients with PD and causes neuroinflammation. Neuroinflammation involves the activation of immune system cells in the brain and the release of inflammation-related molecules. The Toll Like Receptors TLRs involved in this process recognize pathogens and damaged cells and initiate the immune response. In this study, we aimed to investigate the association of the polymorphic allele on the TollIL-1 receptor-associated protein TIRAP gene region, an adaptor protein involved in the TLR signaling pathway, in patients with PD and in the control group. Material and Method 39 PD patients and 40 healthy individuals participated in the study. Blood samples were taken from the participants and DNA isolation was performed. TIRAP rs8177374 975CT polymorphism was determined by PCR and RFLP methods. Results T allele frequency was found to be 0.218 in PD patients and 0.200 in the control group. C allele frequency and CC genotype frequency were found to be high in both groups. F value was found to be 0,128 in PD patients and 0,250 in the control group. OR value was 1.115; CI value was 0,517-2,402. Total OR1,508; P0,758. Conclusion No statistically significant association was found between TIRAP polymorphism and PD. It was concluded that TIRAP polymorphism is not a risk factor in PD patients.

 Objective Parkinsons disease PD is a progressive disease characterized by the loss of dopamine-producing cells in the brain. A protein called α-synuclein accumulates in the brain tissue of patients with PD and causes neuroinflammation. Neuroinflammation involves the activation of immune system cells in the brain and the release of inflammation-related molecules. The Toll Like Receptors TLRs involved in this process recognize pathogens and damaged cells and initiate the immune response. In this study, we aimed to investigate the association of the polymorphic allele on the TollIL-1 receptor-associated protein TIRAP gene region, an adaptor protein involved in the TLR signaling pathway, in patients with PD and in the control group. Material and Method 39 PD patients and 40 healthy individuals participated in the study. Blood samples were taken from the participants and DNA isolation was performed. TIRAP rs8177374 975CT polymorphism was determined by PCR and RFLP methods. Results T allele frequency was found to be 0.218 in PD patients and 0.200 in the control group. C allele frequency and CC genotype frequency were found to be high in both groups. F value was found to be 0,128 in PD patients and 0,250 in the control group. OR value was 1.115; CI value was 0,517-2,402. Total OR1,508; P0,758. Conclusion No statistically significant association was found between TIRAP polymorphism and PD. It was concluded that TIRAP polymorphism is not a risk factor in PD patients.

 Amaç Parkinson hastalığı PH, beyinde dopamin üreten hücrelerin kaybıyla ilerleyen bir hastalıktır. PHli hastaların beyin dokularında α-sinüklein adlı bir protein birikir ve nöroinflamasyona neden olur. Nöroinflamasyon, beyindeki bağışıklık sistemi hücrelerinin aktivasyonu ve iltihaplanma ile ilgili moleküllerin salınımını içerir. Bu süreçte rol oynayan Toll Like Reseptör TLRler patojenleri ve hasarlı hücreleri tanıyarak bağışıklık yanıtını başlatır. Bu çalışmada, TLR sinyal yolağında yer alan bir adaptör protein olan TollIL-1 reseptör ilişkili protein TIRAP gen bölgesi üzerindeki polimorfik allelin PHli hastalarda ve kontrol grubunda karşılaştırmalı olarak ilişkisini araştırmayı amaçladık. Gereç ve Yöntem Çalışmaya 39 PH hastası ve 40 sağlıklı kişi katıldı. Katılımcılardan kan örnekleri alınarak DNA izolasyonu yapıldı. TIRAP rs8177374 975CT polimorfizmi PCR ve RFLP yöntemleri ile belirlendi. Bulgular T allel frekansı PH hastalarında 0,218; kontrol grubunda ise 0,200 olarak bulundu. C allel frekansı ve CC genotip frekansı her iki grupta da yüksek bulundu. F değeri PH hastalarında 0,128; kontrol grubunda ise 0,250 olarak bulundu. OR değeri 1,115; CI değeri ise 0,517-2,402 olarak bulundu. Total OR1,508; P0,758 olarak bulundu. Sonuç TIRAP polimorfizmi ile PH arasında istatistiksel olarak anlamlı bir ilişki bulunmadı. TIRAP polimorfizminin PH hastalarında risk faktörü olmadığı sonucuna varıldı.

Makale detayları

INVESTIGATION OF THE RELATIONSHIP BETWEEN PARKINSON'S DISEASE AND TIRAP GENE POLYMORPHISM

Yazarlar:

Merve Kır Kayan | Nilüfer Şahin Calapoğlu

Yayıncı:

Süleyman Demirel University

Ana Başlıklar:

Genomics

Dergi:

Medical Journal of Süleyman Demirel University Vol.31 Issue 1, pp.103-109

Belge Tipi:

Makale

Yayın Tarihi:

3/2024

Belge Dili:

Türkçe

Document ID (Org No.):

2001313405

pISSN:

1300-7416

Toll Like Receptor

Neuroinflammation

TIRAP

ParkinsonParkinson Hastalığı

Toll Like Reseptör

Nöroinflamasyon

TIRAP

Sayı

Makale detayları

Yazarlar: Merve Kır Kayan | Nilüfer Şahin Calapoğlu

Yayıncı: Süleyman Demirel University

Ana Başlıklar: Genomics

Dergi: Medical Journal of Süleyman Demirel University Vol.31 Issue 1, pp.103-109

Belge Tipi: Makale

Yayın Tarihi: 3/2024

Belge Dili: Türkçe

Document ID (Org No.): 2001313405

pISSN: 1300-7416

Sayı

Yazarlar:

Merve Kır Kayan | Nilüfer Şahin Calapoğlu

Yayıncı:

Süleyman Demirel University

Ana Başlıklar:

Genomics

Dergi:

Medical Journal of Süleyman Demirel University Vol.31 Issue 1, pp.103-109

Belge Tipi:

Makale

Yayın Tarihi:

3/2024

Belge Dili:

Türkçe

Document ID (Org No.):

2001313405

pISSN:

1300-7416