Real-time PCR using high-resolution melting analysis technology for diagnosis of  Leishmania  and determination of types of clinical samples

Serhat SİREKBASAN; Erdal POLAT

Makale detayları

Real-time PCR using high-resolution melting analysis technology for diagnosis of Leishmania and determination of types of clinical samples

Yazarlar:

Serhat SİREKBASAN | Erdal POLAT

Yayıncı:

TUBITAK

Ana Başlıklar:

Sağlık teşvikleri

Dergi:

Turkish Journal of Medical Sciences 2018 Vol.48 Issue 6, pp.1358-1363

Belge Tipi:

Makale

Yayın Tarihi:

12/2018

Belge Dili:

İngilizce

Document ID (Org No.):

2000750523

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

Özet:

 


 
                         Background/aim: 
Leishmaniasis is a disease group carried by 
Phlebotomus
 and 
Lutzomyia
 sand flies infected with 
Leishmania
 and is 
mostly  observed  in  rural  areas.  In  this  study,  using  high-resolution  melting  analysis  (HRMA),  we  aim  to  identify  the  active  types  of  
leishmaniasis, which are inadequately identified by classical methods. 
Materials  and  methods:  
Samples  of  85  patients  were  examined  in  the  study.  Six  of  the  patients  were  suspected  of  having  visceral  
leishmaniasis  (VL)  and  79  cutaneous  leishmaniasis  (CL).  The  slides  prepared  from  the  samples  were  stained  with  Giemsa  stain  and  
examined under a light microscope. The results were compared with those determined in real-time PCR. When the real-time PCR result 
was positive for 
Leishmania
, we determined the type by HRMA.
Results: 
Among  85  
Leishmania
  amastigote  samples,  28  (32.9%)  of  them  were  detected  and  accepted  as  positive  by  microscopic  
examination.  On  the  other  hand,  25  (29.4%)  of  the  85  samples  were  found  as  positive  using  real-time  PCR.  In  addition,  when  25  
Leishmania
-positive samples were examined by HRMA, the results showed that 21 (84%) were 
L. tropica
, 3 (12%) were 
L. major
, and 1 
(4%) was 
L. infantum
.
Conclusion: 
Based on our findings, we conclude that using real-time PCR and HRMA is useful in determining the cause of the disease 
in leishmaniasis.
                    

.

Makale detayları

Real-time PCR using high-resolution melting analysis technology for diagnosis of Leishmania and determination of types of clinical samples

Yazarlar: Serhat SİREKBASAN | Erdal POLAT

Yayıncı: TUBITAK

Ana Başlıklar: Sağlık teşvikleri

Dergi: Turkish Journal of Medical Sciences 2018 Vol.48 Issue 6, pp.1358-1363

Belge Tipi: Makale

Yayın Tarihi: 12/2018

Belge Dili: İngilizce

Document ID (Org No.): 2000750523

Sıklık: İki Aylık

eISSN: 1303-6165

pISSN: 1300-0144

Sayı

Yazarlar:

Serhat SİREKBASAN | Erdal POLAT

Yayıncı:

TUBITAK

Ana Başlıklar:

Sağlık teşvikleri

Dergi:

Turkish Journal of Medical Sciences 2018 Vol.48 Issue 6, pp.1358-1363

Belge Tipi:

Makale

Yayın Tarihi:

12/2018

Belge Dili:

İngilizce

Document ID (Org No.):

2000750523

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144