Marker-assisted selection for rice brown planthopper (Nilaparvata lugens)resistance using linked SSR markers

MAHMOODREZA SHABANIMOFRAD; MOHD RAFII YUSOP; SADEGH ASHKANI; MOHAMED HANAFI MUSA; NUR AZURA ADAM; IFFAH HAIFA; ABDUL RAHIM HARUN; MOHAMMAD ABDUL LATIF

Makale detayları

Marker-assisted selection for rice brown planthopper (Nilaparvata lugens)resistance using linked SSR markers

Yazarlar:

MAHMOODREZA SHABANIMOFRAD | MOHD RAFII YUSOP | SADEGH ASHKANI | MOHAMED HANAFI MUSA | NUR AZURA ADAM | IFFAH HAIFA | ABDUL RAHIM HARUN | MOHAMMAD ABDUL LATIF

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2015 Vol.39 Issue 5, pp.666-673

Belge Tipi:

Makale

Yayın Tarihi:

12/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000811833

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Anahtar kelimeler:

Özet:

 

Abstract: Developing cultivars resistant against different biotypes of brown planthopper (BPH) through marker-assisted selection (MAS) is an effective management strategy to control this pest. In this study, 28 polymorphic simple sequence repeat (SSR) markers were analyzed in 108 F3 progenies derived from the cross of Rathu Heenati and MR276 rice cultivars to investigate the association with BPH resistance against biotypes 2 and 3. For statistical analysis, parents and their offspring were grouped into 2 phenotypic classes based on their levels of BPH resistance. Chi-square analysis demonstrated a good fit to a ratio of 3:1 for the segregation of resistance and susceptibility. Association of SSR markers with phenotypic traits in F3 progenies were identified. Six SSR markers (RM401, RM5953, RM217, RM210, RM242, and RM1103) were significantly associated with BPH resistance to biotypes 2 and 3 of BPH. Out of these 6 markers, RM401, RM5953, and RM217 accounted for about 17% of total phenotypic variation and RM210, RM242, and RM1103 accounted for 20% of total phenotypic variation against biotypes 2 and 3, respectively. Therefore, the above 6 markers were confirmed for MAS in rice breeding programs to develop BPH-resistant rice cultivars.

 

Abstract: Developing cultivars resistant against different biotypes of brown planthopper (BPH) through marker-assisted selection (MAS) is an effective management strategy to control this pest. In this study, 28 polymorphic simple sequence repeat (SSR) markers were analyzed in 108 F3 progenies derived from the cross of Rathu Heenati and MR276 rice cultivars to investigate the association with BPH resistance against biotypes 2 and 3. For statistical analysis, parents and their offspring were grouped into 2 phenotypic classes based on their levels of BPH resistance. Chi-square analysis demonstrated a good fit to a ratio of 3:1 for the segregation of resistance and susceptibility. Association of SSR markers with phenotypic traits in F3 progenies were identified. Six SSR markers (RM401, RM5953, RM217, RM210, RM242, and RM1103) were significantly associated with BPH resistance to biotypes 2 and 3 of BPH. Out of these 6 markers, RM401, RM5953, and RM217 accounted for about 17% of total phenotypic variation and RM210, RM242, and RM1103 accounted for 20% of total phenotypic variation against biotypes 2 and 3, respectively. Therefore, the above 6 markers were confirmed for MAS in rice breeding programs to develop BPH-resistant rice cultivars.

Makale detayları

Marker-assisted selection for rice brown planthopper (Nilaparvata lugens)resistance using linked SSR markers

Yazarlar:

MAHMOODREZA SHABANIMOFRAD | MOHD RAFII YUSOP | SADEGH ASHKANI | MOHAMED HANAFI MUSA | NUR AZURA ADAM | IFFAH HAIFA | ABDUL RAHIM HARUN | MOHAMMAD ABDUL LATIF

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2015 Vol.39 Issue 5, pp.666-673

Belge Tipi:

Makale

Yayın Tarihi:

12/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000811833

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Bph resistance gene

F3 progenies

microsatellites

rice

Sayı

Makale detayları

Marker-assisted selection for rice brown planthopper (Nilaparvata lugens)resistance using linked SSR markers

Yazarlar: MAHMOODREZA SHABANIMOFRAD | MOHD RAFII YUSOP | SADEGH ASHKANI | MOHAMED HANAFI MUSA | NUR AZURA ADAM | IFFAH HAIFA | ABDUL RAHIM HARUN | MOHAMMAD ABDUL LATIF

Yayıncı: TUBITAK

Dergi: Turkish Journal of Biology 2015 Vol.39 Issue 5, pp.666-673

Belge Tipi: Makale

Yayın Tarihi: 12/2015

Belge Dili: Türkçe

Document ID (Org No.): 2000811833

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

Bph resistance gene

F3 progenies

microsatellites

rice

Sayı

Yazarlar:

MAHMOODREZA SHABANIMOFRAD | MOHD RAFII YUSOP | SADEGH ASHKANI | MOHAMED HANAFI MUSA | NUR AZURA ADAM | IFFAH HAIFA | ABDUL RAHIM HARUN | MOHAMMAD ABDUL LATIF

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2015 Vol.39 Issue 5, pp.666-673

Belge Tipi:

Makale

Yayın Tarihi:

12/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000811833

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152