Determination of Molnupiravir, the Active Pharmaceutical Substance Used in Covid-19 Disease, by Electrochemical Methods

Mehmet Işıklı; Hilal Çelik Kazıcı

Makale detayları

Determination of Molnupiravir, the Active Pharmaceutical Substance Used in Covid-19 Disease, by Electrochemical Methods

Yazarlar:

Mehmet Işıklı | Hilal Çelik Kazıcı

Yayıncı:

Van Yuzuncu Yıl University

Ana Başlıklar:

Sensör Teknolojisi | Elektrokimyasal Teknolojiler | Malzeme Bilimi ve Teknolojileri

Dergi:

Yüzüncü Yıl Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.30 Issue 2, pp.752-770

Belge Tipi:

Makale

Yayın Tarihi:

8/2025

Belge Dili:

Türkçe

Document ID (Org No.):

2001357531

pISSN:

1300-5413

Anahtar kelimeler:

Özet:

 The mutation capacity and unpredictable spread of SARS-CoV-2 complicate the control of the infection and increase the need for advanced sensor technologies for the sensitive analysis of antiviral drugs. In this study, the electrochemical behavior and sensitive voltammetric determination of MLP were described for the first time using multi-walled carbon nanotube MWCNT supported ferrite nanoparticle modified glassy carbon electrode CrFe2O4MWCNT prepared by coprecipitation method. The prepared CrFe₂O₄ nanoparticles were characterized by transmission electron microscope TEM images, energy dispersive X-ray SEMEDX and X-ray diffraction XRD spectra. Cyclic voltammetry CV measurements showed good electrochemical activity of MWCNT supported ferrite nanoparticles against oxidation of MLP in 0.1M phosphate buffer. Due to the high electrochemical activity of CrFe₂O₄ nanoparticles, the peak current of MLP showed a significant increase in CrFe₂O₄MWCNT electrode compared to the plain glassy carbon electrode. According to differential pulse voltammetry DPV studies, the developed electrode exhibited a wide linear range LR and provided successful results with high sensitivity and low detection limit LOD. Within the scope of interference studies, it was observed that the performance of the sensor was not significantly affected in the presence of biomolecules such as dopamine DA, ascorbic acid AA, Glucose Gl and uric acid UA and ions such as K, Na, Cl-. As a result of stability studies, it was determined that the sensor maintained its stability for 3 days. In conclusion, the developed CrFe₂O₄ MWCNT based electrochemical sensor provides high sensitivity, selectivity and stability in MLP analysis, thus providing a strong alternative to existing methods.

 The mutation capacity and unpredictable spread of SARS-CoV-2 complicate the control of the infection and increase the need for advanced sensor technologies for the sensitive analysis of antiviral drugs. In this study, the electrochemical behavior and sensitive voltammetric determination of MLP were described for the first time using multi-walled carbon nanotube MWCNT supported ferrite nanoparticle modified glassy carbon electrode CrFe2O4MWCNT prepared by coprecipitation method. The prepared CrFe₂O₄ nanoparticles were characterized by transmission electron microscope TEM images, energy dispersive X-ray SEMEDX and X-ray diffraction XRD spectra. Cyclic voltammetry CV measurements showed good electrochemical activity of MWCNT supported ferrite nanoparticles against oxidation of MLP in 0.1M phosphate buffer. Due to the high electrochemical activity of CrFe₂O₄ nanoparticles, the peak current of MLP showed a significant increase in CrFe₂O₄MWCNT electrode compared to the plain glassy carbon electrode. According to differential pulse voltammetry DPV studies, the developed electrode exhibited a wide linear range LR and provided successful results with high sensitivity and low detection limit LOD. Within the scope of interference studies, it was observed that the performance of the sensor was not significantly affected in the presence of biomolecules such as dopamine DA, ascorbic acid AA, Glucose Gl and uric acid UA and ions such as K, Na, Cl-. As a result of stability studies, it was determined that the sensor maintained its stability for 3 days. In conclusion, the developed CrFe₂O₄ MWCNT based electrochemical sensor provides high sensitivity, selectivity and stability in MLP analysis, thus providing a strong alternative to existing methods.

 The mutation capacity and unpredictable spread of SARS-CoV-2 complicate the control of the infection and increase the need for advanced sensor technologies for the sensitive analysis of antiviral drugs. In this study, the electrochemical behavior and sensitive voltammetric determination of MLP were described for the first time using multi-walled carbon nanotube MWCNT supported ferrite nanoparticle modified glassy carbon electrode CrFe2O4MWCNT prepared by coprecipitation method. The prepared CrFe₂O₄ nanoparticles were characterized by transmission electron microscope TEM images, energy dispersive X-ray SEMEDX and X-ray diffraction XRD spectra. Cyclic voltammetry CV measurements showed good electrochemical activity of MWCNT supported ferrite nanoparticles against oxidation of MLP in 0.1M phosphate buffer. Due to the high electrochemical activity of CrFe₂O₄ nanoparticles, the peak current of MLP showed a significant increase in CrFe₂O₄MWCNT electrode compared to the plain glassy carbon electrode. According to differential pulse voltammetry DPV studies, the developed electrode exhibited a wide linear range LR and provided successful results with high sensitivity and low detection limit LOD. Within the scope of interference studies, it was observed that the performance of the sensor was not significantly affected in the presence of biomolecules such as dopamine DA, ascorbic acid AA, Glucose Gl and uric acid UA and ions such as K, Na, Cl-. As a result of stability studies, it was determined that the sensor maintained its stability for 3 days. In conclusion, the developed CrFe₂O₄ MWCNT based electrochemical sensor provides high sensitivity, selectivity and stability in MLP analysis, thus providing a strong alternative to existing methods.

 SARS-CoV-2nin mutasyon kapasitesi ve öngörülemeyen yayılımı, enfeksiyonun kontrol altına alınmasını zorlaştırmakta ve antiviral ilaçların hassas analizine yönelik gelişmiş sensör teknolojilerine duyulan ihtiyacı artırmaktadır. Bu çalışmada, birlikte çöktürme yöntemiyle hazırlanmış çok duvarlı karbon nanotüp MWCNT destekli ferrit nanopartikül modifiye camımsı karbon elektrodu CrFe2O4MWCNT kullanılarak MLPnin elektrokimyasal davranışı ve hassas voltammetrik tayini ilk kez tanımlanmıştır. Hazırlanan CrFe₂O₄ nanopartikülleri, transmisyon elektron mikroskobu TEM görüntüleri, enerji dağılımlı X-ışını SEMEDX ve X-ışını kırınımı XRD spektrumları ile karakterize edilmiştir. Döngüsel voltammetri CV ölçümleri, MWCNT destekli ferrit nanopartiküllerinin MLPnin 0.1M fosfat tamponunda oksidasyonuna karşı iyi bir elektrokimyasal aktivite göstermiştir. CrFe₂O₄ nanopartiküllerinin yüksek elektrokimyasal aktivitesi sayesinde, MLPnin pik akımı, yalın camımsı karbon elektrot ile karşılaştırıldığında CrFe₂O₄MWCNT elektrodunda belirgin bir artış göstermiştir. Diferansiyel puls voltammetri DPV çalışmalarına göre, geliştirilen elektrot geniş bir doğrusal aralık LR sergileyerek yüksek duyarlılık ve düşük tespit sınırı LOD ile başarılı sonuçlar elde edilmesini sağlamıştır. Girişim çalışmaları kapsamında, dopamin DA, askorbik asit AA, Glukoz Gl ve ürik asit UA gibi biyomoleküllerin ve K, Na, Cl- gibi iyonların varlığında sensörün performansının önemli ölçüde etkilenmediği gözlemlenmiştir. Stabilite çalışmaları sonucunda, sensörün 3 gün boyunca kararlılığını koruduğu belirlenmiştir. Sonuç olarak, geliştirilen MWCNT destekli CrFe₂O₄ tabanlı elektrokimyasal sensör, MLP analizinde yüksek hassasiyet, seçicilik ve stabilite sunarak mevcut yöntemlere kıyasla güçlü bir alternatif oluşturmuştur.

Makale detayları

Determination of Molnupiravir, the Active Pharmaceutical Substance Used in Covid-19 Disease, by Electrochemical Methods

Yazarlar:

Mehmet Işıklı | Hilal Çelik Kazıcı

Yayıncı:

Van Yuzuncu Yıl University

Ana Başlıklar:

Sensör Teknolojisi | Elektrokimyasal Teknolojiler | Malzeme Bilimi ve Teknolojileri

Dergi:

Yüzüncü Yıl Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.30 Issue 2, pp.752-770

Belge Tipi:

Makale

Yayın Tarihi:

8/2025

Belge Dili:

Türkçe

Document ID (Org No.):

2001357531

pISSN:

1300-5413

Electrochemical sensor

Molnupiravir (MLP)

SARS-CoV-2Elektrokimyasal sensör

Molnupiravir (MLP)

SARS-CoV-2

Sayı

Makale detayları

Determination of Molnupiravir, the Active Pharmaceutical Substance Used in Covid-19 Disease, by Electrochemical Methods

Yazarlar: Mehmet Işıklı | Hilal Çelik Kazıcı

Yayıncı: Van Yuzuncu Yıl University

Ana Başlıklar: Sensör Teknolojisi | Elektrokimyasal Teknolojiler | Malzeme Bilimi ve Teknolojileri

Dergi: Yüzüncü Yıl Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.30 Issue 2, pp.752-770

Belge Tipi: Makale

Yayın Tarihi: 8/2025

Belge Dili: Türkçe

Document ID (Org No.): 2001357531

pISSN: 1300-5413

Electrochemical sensor

Molnupiravir (MLP)

SARS-CoV-2Elektrokimyasal sensör

Molnupiravir (MLP)

SARS-CoV-2

Sayı

Yazarlar:

Mehmet Işıklı | Hilal Çelik Kazıcı

Yayıncı:

Van Yuzuncu Yıl University

Ana Başlıklar:

Sensör Teknolojisi | Elektrokimyasal Teknolojiler | Malzeme Bilimi ve Teknolojileri

Dergi:

Yüzüncü Yıl Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.30 Issue 2, pp.752-770

Belge Tipi:

Makale

Yayın Tarihi:

8/2025

Belge Dili:

Türkçe

Document ID (Org No.):

2001357531

pISSN:

1300-5413