Optimization of ultrasound-assisted extraction of protein from the byproduct of the hazelnut oil industry using reverse micelles

Elif Meltem İşçimen; Mehmet Hayta

Makale detayları

Optimization of ultrasound-assisted extraction of protein from the byproduct of the hazelnut oil industry using reverse micelles

Yazarlar:

Elif Meltem İşçimen | Mehmet Hayta

Yayıncı:

Yildiz Technical University

Ana Başlıklar:

Clinical Chemistry

Dergi:

Sigma Journal of Engineering and Natural Sciences Vol.42 Issue 4, pp.1202-1213

Belge Tipi:

Makale

Yayın Tarihi:

1/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001324618

pISSN:

1304-7191

Anahtar kelimeler:

Özet:

 In the current research, an ultrasound-assisted extraction UAE procedure was established for the protein extracted employing a reverse micelles system RMS from the by-product of the hazelnut oil industry. The optimum extraction circumstances in the UAE were identified as a dioctyl sodium sulfosuccinate AOT an amount of 0.05 gmL, a water content of 25.2 W0, a 0.02 gmL solid-to-liquid ratio, ultrasound time of 17.52 min, ultrasound cycle 1, and ultrasound power 80 using the response surface approach. Under ideal circumstances, the maximum yield was recorded as 44.84 mg BSAg of hazelnut meal protein HMP for RMS, it had a higher yield obtained by alkaline solution AS. α-helix, β-turn, and β-sheet structures of HMP increased while the random coil decreased as evidenced by FTIR and SEM images proving that the cell walls were destructed and had more cracks in RMS. Overall, the find-ings indicated that UAE combined with RMS might be an effective approach for extracting protein from HMP and is likely to lead to an alternative evaluation possibility of an industrial by-product.

 In the current research, an ultrasound-assisted extraction UAE procedure was established for the protein extracted employing a reverse micelles system RMS from the by-product of the hazelnut oil industry. The optimum extraction circumstances in the UAE were identified as a dioctyl sodium sulfosuccinate AOT an amount of 0.05 gmL, a water content of 25.2 W0, a 0.02 gmL solid-to-liquid ratio, ultrasound time of 17.52 min, ultrasound cycle 1, and ultrasound power 80 using the response surface approach. Under ideal circumstances, the maximum yield was recorded as 44.84 mg BSAg of hazelnut meal protein HMP for RMS, it had a higher yield obtained by alkaline solution AS. α-helix, β-turn, and β-sheet structures of HMP increased while the random coil decreased as evidenced by FTIR and SEM images proving that the cell walls were destructed and had more cracks in RMS. Overall, the find-ings indicated that UAE combined with RMS might be an effective approach for extracting protein from HMP and is likely to lead to an alternative evaluation possibility of an industrial by-product.

 In the current research, an ultrasound-assisted extraction UAE procedure was established for the protein extracted employing a reverse micelles system RMS from the by-product of the hazelnut oil industry. The optimum extraction circumstances in the UAE were identified as a dioctyl sodium sulfosuccinate AOT an amount of 0.05 gmL, a water content of 25.2 W0, a 0.02 gmL solid-to-liquid ratio, ultrasound time of 17.52 min, ultrasound cycle 1, and ultrasound power 80 using the response surface approach. Under ideal circumstances, the maximum yield was recorded as 44.84 mg BSAg of hazelnut meal protein HMP for RMS, it had a higher yield obtained by alkaline solution AS. α-helix, β-turn, and β-sheet structures of HMP increased while the random coil decreased as evidenced by FTIR and SEM images proving that the cell walls were destructed and had more cracks in RMS. Overall, the find-ings indicated that UAE combined with RMS might be an effective approach for extracting protein from HMP and is likely to lead to an alternative evaluation possibility of an industrial by-product.

 In the current research, an ultrasound-assisted extraction UAE procedure was established for the protein extracted employing a reverse micelles system RMS from the by-product of the hazelnut oil industry. The optimum extraction circumstances in the UAE were identified as a dioctyl sodium sulfosuccinate AOT an amount of 0.05 gmL, a water content of 25.2 W0, a 0.02 gmL solid-to-liquid ratio, ultrasound time of 17.52 min, ultrasound cycle 1, and ultrasound power 80 using the response surface approach. Under ideal circumstances, the maximum yield was recorded as 44.84 mg BSAg of hazelnut meal protein HMP for RMS, it had a higher yield obtained by alkaline solution AS. α-helix, β-turn, and β-sheet structures of HMP increased while the random coil decreased as evidenced by FTIR and SEM images proving that the cell walls were destructed and had more cracks in RMS. Overall, the find-ings indicated that UAE combined with RMS might be an effective approach for extracting protein from HMP and is likely to lead to an alternative evaluation possibility of an industrial by-product.

Makale detayları

Optimization of ultrasound-assisted extraction of protein from the byproduct of the hazelnut oil industry using reverse micelles

Yazarlar:

Elif Meltem İşçimen | Mehmet Hayta

Yayıncı:

Yildiz Technical University

Ana Başlıklar:

Clinical Chemistry

Dergi:

Sigma Journal of Engineering and Natural Sciences Vol.42 Issue 4, pp.1202-1213

Belge Tipi:

Makale

Yayın Tarihi:

1/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001324618

pISSN:

1304-7191

AOT

Hazelnut Waste

FTIR

Optimization

Ultrasound

Sayı

Makale detayları

Optimization of ultrasound-assisted extraction of protein from the byproduct of the hazelnut oil industry using reverse micelles

Yazarlar: Elif Meltem İşçimen | Mehmet Hayta

Yayıncı: Yildiz Technical University

Ana Başlıklar: Clinical Chemistry

Dergi: Sigma Journal of Engineering and Natural Sciences Vol.42 Issue 4, pp.1202-1213

Belge Tipi: Makale

Yayın Tarihi: 1/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001324618

pISSN: 1304-7191

AOT

Hazelnut Waste

FTIR

Optimization

Ultrasound

Sayı

Yazarlar:

Elif Meltem İşçimen | Mehmet Hayta

Yayıncı:

Yildiz Technical University

Ana Başlıklar:

Clinical Chemistry

Dergi:

Sigma Journal of Engineering and Natural Sciences Vol.42 Issue 4, pp.1202-1213

Belge Tipi:

Makale

Yayın Tarihi:

1/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001324618

pISSN:

1304-7191