Nuclear receptor agonist activity studies on some Plantago species and Scutellaria salviifolia Benth.: A particular focus on liver x receptor alpha and retinoid x receptor alpha connected with the inflammation process

Vahap Murat Kutluay; Yasin Genç; Zeynep Doğan; İclal Saraçoğlu

Makale detayları

Nuclear receptor agonist activity studies on some Plantago species and Scutellaria salviifolia Benth.: A particular focus on liver x receptor alpha and retinoid x receptor alpha connected with the inflammation process

Yazarlar:

Vahap Murat Kutluay | Yasin Genç | Zeynep Doğan | İclal Saraçoğlu

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmacognosy

Dergi:

Journal of Research in Pharmacy Vol.26 Issue 2, pp.272-278

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001346335

pISSN:

2630-6344

Anahtar kelimeler:

Özet:

 Plantago, Scutellaria, liver Nuclear receptors are a superfamily of transcription factors that regulate gene transcription and intracellular function. They have a key role in various biological processes. Liver X receptors LXR mainly play role in lipid metabolism, cholesterol synthesis, glucose homeostasis, and inflammation whereas retinoid X receptor alpha RXRα plays important role in inflammation and immunity. In this study, Plantago holosteum, P. major subsp. major, P. lagopus, P. scabra, and Scutellaria salviifolia were selected for further investigation due to their anti-inflammatory activities. For this purpose, liver x receptor alpha LXRα and retinoid x receptor alpha RXRα agonist activity of the extracts was determined through luciferase reporter gene assay on HEK293 cells at a concentration of 100 µgml. P. holosteum, P. major subsp. major, P. lagopus, and P. scabra extracts showed weak LXRα agonist activity with the fold values in a range of 0.95-1.39. P. major showed the highest agonist activity among tested Plantago species. For LXRα agonist activity, S. salviifolia extract had a fold value of 2.37 which was comparable to the positive control T09011317 at 10 nM concentration. Results for RXRα agonist activity found to be similar to LXRα for all extracts. S. salviifolia showed the highest RXRα agonist activity with a fold value of 1.62. Results suggested that Scutellaria species, which are used as traditional medicine especially in eastern Asia due to its anti-inflammatory effects, might show this effect due to LXRα activation. x receptor,retinoid x receptor, anti-inflammatory.

 Plantago, Scutellaria, liver Nuclear receptors are a superfamily of transcription factors that regulate gene transcription and intracellular function. They have a key role in various biological processes. Liver X receptors LXR mainly play role in lipid metabolism, cholesterol synthesis, glucose homeostasis, and inflammation whereas retinoid X receptor alpha RXRα plays important role in inflammation and immunity. In this study, Plantago holosteum, P. major subsp. major, P. lagopus, P. scabra, and Scutellaria salviifolia were selected for further investigation due to their anti-inflammatory activities. For this purpose, liver x receptor alpha LXRα and retinoid x receptor alpha RXRα agonist activity of the extracts was determined through luciferase reporter gene assay on HEK293 cells at a concentration of 100 µgml. P. holosteum, P. major subsp. major, P. lagopus, and P. scabra extracts showed weak LXRα agonist activity with the fold values in a range of 0.95-1.39. P. major showed the highest agonist activity among tested Plantago species. For LXRα agonist activity, S. salviifolia extract had a fold value of 2.37 which was comparable to the positive control T09011317 at 10 nM concentration. Results for RXRα agonist activity found to be similar to LXRα for all extracts. S. salviifolia showed the highest RXRα agonist activity with a fold value of 1.62. Results suggested that Scutellaria species, which are used as traditional medicine especially in eastern Asia due to its anti-inflammatory effects, might show this effect due to LXRα activation. x receptor,retinoid x receptor, anti-inflammatory.

 Plantago, Scutellaria, liver Nuclear receptors are a superfamily of transcription factors that regulate gene transcription and intracellular function. They have a key role in various biological processes. Liver X receptors LXR mainly play role in lipid metabolism, cholesterol synthesis, glucose homeostasis, and inflammation whereas retinoid X receptor alpha RXRα plays important role in inflammation and immunity. In this study, Plantago holosteum, P. major subsp. major, P. lagopus, P. scabra, and Scutellaria salviifolia were selected for further investigation due to their anti-inflammatory activities. For this purpose, liver x receptor alpha LXRα and retinoid x receptor alpha RXRα agonist activity of the extracts was determined through luciferase reporter gene assay on HEK293 cells at a concentration of 100 µgml. P. holosteum, P. major subsp. major, P. lagopus, and P. scabra extracts showed weak LXRα agonist activity with the fold values in a range of 0.95-1.39. P. major showed the highest agonist activity among tested Plantago species. For LXRα agonist activity, S. salviifolia extract had a fold value of 2.37 which was comparable to the positive control T09011317 at 10 nM concentration. Results for RXRα agonist activity found to be similar to LXRα for all extracts. S. salviifolia showed the highest RXRα agonist activity with a fold value of 1.62. Results suggested that Scutellaria species, which are used as traditional medicine especially in eastern Asia due to its anti-inflammatory effects, might show this effect due to LXRα activation. x receptor,retinoid x receptor, anti-inflammatory.

 Plantago, Scutellaria, liver Nuclear receptors are a superfamily of transcription factors that regulate gene transcription and intracellular function. They have a key role in various biological processes. Liver X receptors LXR mainly play role in lipid metabolism, cholesterol synthesis, glucose homeostasis, and inflammation whereas retinoid X receptor alpha RXRα plays important role in inflammation and immunity. In this study, Plantago holosteum, P. major subsp. major, P. lagopus, P. scabra, and Scutellaria salviifolia were selected for further investigation due to their anti-inflammatory activities. For this purpose, liver x receptor alpha LXRα and retinoid x receptor alpha RXRα agonist activity of the extracts was determined through luciferase reporter gene assay on HEK293 cells at a concentration of 100 µgml. P. holosteum, P. major subsp. major, P. lagopus, and P. scabra extracts showed weak LXRα agonist activity with the fold values in a range of 0.95-1.39. P. major showed the highest agonist activity among tested Plantago species. For LXRα agonist activity, S. salviifolia extract had a fold value of 2.37 which was comparable to the positive control T09011317 at 10 nM concentration. Results for RXRα agonist activity found to be similar to LXRα for all extracts. S. salviifolia showed the highest RXRα agonist activity with a fold value of 1.62. Results suggested that Scutellaria species, which are used as traditional medicine especially in eastern Asia due to its anti-inflammatory effects, might show this effect due to LXRα activation. x receptor,retinoid x receptor, anti-inflammatory.

Makale detayları

Nuclear receptor agonist activity studies on some Plantago species and Scutellaria salviifolia Benth.: A particular focus on liver x receptor alpha and retinoid x receptor alpha connected with the inflammation process

Yazarlar: Vahap Murat Kutluay | Yasin Genç | Zeynep Doğan | İclal Saraçoğlu

Yayıncı: Marmara University

Ana Başlıklar: Pharmacognosy

Dergi: Journal of Research in Pharmacy Vol.26 Issue 2, pp.272-278

Belge Tipi: Makale

Yayın Tarihi: 6/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001346335

pISSN: 2630-6344

Plantago

Scutellaria

liver x receptor

retinoid x receptor

anti-inflammatory

Sayı

Yazarlar:

Vahap Murat Kutluay | Yasin Genç | Zeynep Doğan | İclal Saraçoğlu

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmacognosy

Dergi:

Journal of Research in Pharmacy Vol.26 Issue 2, pp.272-278

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001346335

pISSN:

2630-6344