EFFECT of CURCUMIN on BREAST CANCER CELLS THROUGH miR-145-5p AND ITS TARGET GENES

Asmaa Abuaisha; Murat Kaya; İlknur Suer; Selman Emiroğlu; Fahrünnisa Abanoz; Şükrü Palanduz; Kıvanç Çefle; Şükrü Öztürk

Makale detayları

EFFECT of CURCUMIN on BREAST CANCER CELLS THROUGH miR-145-5p AND ITS TARGET GENES

Yazarlar:

Asmaa Abuaisha | Murat Kaya | İlknur Suer | Selman Emiroğlu | Fahrünnisa Abanoz | Şükrü Palanduz | Kıvanç Çefle | Şükrü Öztürk

Yayıncı:

Istanbul University

Ana Başlıklar:

Sağlık Hizmetleri ve Sistemleri (Diğer)

Dergi:

İstanbul Tıp Fakültesi Dergisi Vol.87 Issue 3, pp.235-245

Belge Tipi:

Makale

Yayın Tarihi:

7/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001330653

pISSN:

1305-6441

Anahtar kelimeler:

Özet:

 Objective: Curcumin is considered an epigenetic regulator with anticancer effects. The micro-RNA miRNA miR-145-5p is a tumor suppressor that shows low expression levels in various cancers, including breast cancer BC. This study aims to investigate whether curcumin inhibits MCF-7 human BC cell line proliferation and migration by regulating miR-145-5p and its possible target genes. Material and Method: MCF-7 cells were treated with curcumin and its solvent control. Additionally, cells were transfected with an miR-145-5p mimic and a non-targeting miRNA mimic. Cell viability was then evaluated, and the scratch wound assay was used to assess cell migration. The study predicts the miR-145- 5p putative target genes by searching for overlapping genes in the miRNet and miRTarBase v8 databases via the overexpressed genes in the BC tissue samples in the Cancer Genome Atlas TCGA datasets. Expression levels of miR-145-5p and the selected genes were detected using the quantitative real-time polymerase chain reaction qRT-PCR. The 2-ΔΔCt method was used for the quantification analysis, with p0.05 being considered statistically significant. Result: Curcumin treatment and overexpression of miR-145- 5p via the transfection of an miR-145-5p mimic significantly decreased the proliferation and migration of MCF-7 cells. Moreover, curcumin treatment significantly increased the expression of miR-145-5p. The possible target genes of miR-145- 5p i.e., MCM2, MMP1, MMP9, EEF1A2 were downregulated in curcumin-treated MCF-7 cells. Additionally, miR-145-5p mimictransfected cells showed low expression levels of the MCM2, MMP1, and MMP9 genes. Conclusion: Curcumin inhibits the proliferation and migration of MCF-7 cells by acting on miR-145-5p and its possible target genes.

 Objective: Curcumin is considered an epigenetic regulator with anticancer effects. The micro-RNA miRNA miR-145-5p is a tumor suppressor that shows low expression levels in various cancers, including breast cancer BC. This study aims to investigate whether curcumin inhibits MCF-7 human BC cell line proliferation and migration by regulating miR-145-5p and its possible target genes. Material and Method: MCF-7 cells were treated with curcumin and its solvent control. Additionally, cells were transfected with an miR-145-5p mimic and a non-targeting miRNA mimic. Cell viability was then evaluated, and the scratch wound assay was used to assess cell migration. The study predicts the miR-145- 5p putative target genes by searching for overlapping genes in the miRNet and miRTarBase v8 databases via the overexpressed genes in the BC tissue samples in the Cancer Genome Atlas TCGA datasets. Expression levels of miR-145-5p and the selected genes were detected using the quantitative real-time polymerase chain reaction qRT-PCR. The 2-ΔΔCt method was used for the quantification analysis, with p0.05 being considered statistically significant. Result: Curcumin treatment and overexpression of miR-145- 5p via the transfection of an miR-145-5p mimic significantly decreased the proliferation and migration of MCF-7 cells. Moreover, curcumin treatment significantly increased the expression of miR-145-5p. The possible target genes of miR-145- 5p i.e., MCM2, MMP1, MMP9, EEF1A2 were downregulated in curcumin-treated MCF-7 cells. Additionally, miR-145-5p mimictransfected cells showed low expression levels of the MCM2, MMP1, and MMP9 genes. Conclusion: Curcumin inhibits the proliferation and migration of MCF-7 cells by acting on miR-145-5p and its possible target genes.

 Objective: Curcumin is considered an epigenetic regulator with anticancer effects. The micro-RNA miRNA miR-145-5p is a tumor suppressor that shows low expression levels in various cancers, including breast cancer BC. This study aims to investigate whether curcumin inhibits MCF-7 human BC cell line proliferation and migration by regulating miR-145-5p and its possible target genes. Material and Method: MCF-7 cells were treated with curcumin and its solvent control. Additionally, cells were transfected with an miR-145-5p mimic and a non-targeting miRNA mimic. Cell viability was then evaluated, and the scratch wound assay was used to assess cell migration. The study predicts the miR-145- 5p putative target genes by searching for overlapping genes in the miRNet and miRTarBase v8 databases via the overexpressed genes in the BC tissue samples in the Cancer Genome Atlas TCGA datasets. Expression levels of miR-145-5p and the selected genes were detected using the quantitative real-time polymerase chain reaction qRT-PCR. The 2-ΔΔCt method was used for the quantification analysis, with p0.05 being considered statistically significant. Result: Curcumin treatment and overexpression of miR-145- 5p via the transfection of an miR-145-5p mimic significantly decreased the proliferation and migration of MCF-7 cells. Moreover, curcumin treatment significantly increased the expression of miR-145-5p. The possible target genes of miR-145- 5p i.e., MCM2, MMP1, MMP9, EEF1A2 were downregulated in curcumin-treated MCF-7 cells. Additionally, miR-145-5p mimictransfected cells showed low expression levels of the MCM2, MMP1, and MMP9 genes. Conclusion: Curcumin inhibits the proliferation and migration of MCF-7 cells by acting on miR-145-5p and its possible target genes.

 Amaç: Kurkumin anti-kanser etkileri olan bir epigenetik regülatör olarak kabul edilmektedir. miR-145-5p, meme kanseri MK dahil olmak üzere bir çok kanserde ekspresyon düzeyi düşük olan bir tümör baskılayıcı mikroRNAdır miRNA. Bu çalışmada, kurkuminin miR-145-5pyi ve olası hedef genlerini regüle ederek MCF-7 insan MK hücre hattının proliferasyonunu ve göçünü inhibe edip etmediğinin araştırılması amaçlanmıştır. Gereç ve Yöntem: MCF-7 hücreleri kurkumin ve onun solvent kontrolü ile muamele edildi. Ayrıca hücreler miR-145-5p mimiği ve negatif kontrol miRNA mimiği ile transfekte edildi. Hücre canlılığı değerlendirildi ve hücre göçü scratch yara wound testi kullanılarak değerlendirildi. miR-145-5pnin potansiyel hedefleri, MK doku örneklerinde aşırı eksprese edilen genlerle miRNet ve miRTarBase v8 veri-tabanları ile örtüşen genlerin Kanser Genom Atlası TCGA datasetlerinde araştırılması yoluyla belirlendi. miR-145-5p ve seçilen genlerin ekspresyon düzeyleri, kantitatif gerçek-zamanlı polimeraz zincir reaksiyonu qRT-PCR ile tespit edildi. Kantifikasyon analizi için 2-ΔΔCt yöntemi kullanıldı. p-değeri 0,05 istatistiksel olarak anlamlı kabul edildi. Bulgular: Hem kurkumin ile muamele edilmiş hem de miR-145-5p mimiği ile transfekte edilmiş MCF-7 hücrelerinde, proliferasyonun ve göçün, kontrollere kıyasla azaldığı gözlenmiştir. Ayrıca, kurkumin ile muamele edilen hücrelerde miR-145-5pnin ekspresyonunun anlamlı düzeyde arttığı görülmüştür. miR-145-5pnin olası hedef genlerinin, MCM2, MMP1, MMP9 ve EEF1A2, kurkumin ile muamele edilmiş MCF-7 hücrelerinde ekspresyon düzeyinin azaldığı saptanmıştır. Bunun yanı sıra, miR-145-5p mimiği ile transfekte edilmiş hücrelerde, MCM2, MMP1 ve MMP9 genlerinin ekspresyon düzeyinin düşük olduğu belirlenmiştir. Sonuç: Kurkuminin, miR-145-5p ve olası hedef genleri üzerinden etki ederek MCF-7 hücrelerinin hem proliferasyonunu hem de göçünü inhibe ettiği gösterilmiştir.

Makale detayları

EFFECT of CURCUMIN on BREAST CANCER CELLS THROUGH miR-145-5p AND ITS TARGET GENES

Yazarlar:

Asmaa Abuaisha | Murat Kaya | İlknur Suer | Selman Emiroğlu | Fahrünnisa Abanoz | Şükrü Palanduz | Kıvanç Çefle | Şükrü Öztürk

Yayıncı:

Istanbul University

Ana Başlıklar:

Sağlık Hizmetleri ve Sistemleri (Diğer)

Dergi:

İstanbul Tıp Fakültesi Dergisi Vol.87 Issue 3, pp.235-245

Belge Tipi:

Makale

Yayın Tarihi:

7/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001330653

pISSN:

1305-6441

Breast cancer

MCF-7 cell line

curcumin

miR-145- 5p

target genesMeme kanseri

MCF-7 hücre hattı

kurkumin

miR-145-5p

hedef genler

Sayı

Makale detayları

Yazarlar: Asmaa Abuaisha | Murat Kaya | İlknur Suer | Selman Emiroğlu | Fahrünnisa Abanoz | Şükrü Palanduz | Kıvanç Çefle | Şükrü Öztürk

Yayıncı: Istanbul University

Ana Başlıklar: Sağlık Hizmetleri ve Sistemleri (Diğer)

Dergi: İstanbul Tıp Fakültesi Dergisi Vol.87 Issue 3, pp.235-245

Belge Tipi: Makale

Yayın Tarihi: 7/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001330653

pISSN: 1305-6441

Sayı

Yazarlar:

Asmaa Abuaisha | Murat Kaya | İlknur Suer | Selman Emiroğlu | Fahrünnisa Abanoz | Şükrü Palanduz | Kıvanç Çefle | Şükrü Öztürk

Yayıncı:

Istanbul University

Ana Başlıklar:

Sağlık Hizmetleri ve Sistemleri (Diğer)

Dergi:

İstanbul Tıp Fakültesi Dergisi Vol.87 Issue 3, pp.235-245

Belge Tipi:

Makale

Yayın Tarihi:

7/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001330653

pISSN:

1305-6441