INVESTIGATION OF DNA GYRASE RESISTANCE GENES IN ACINETOBACTER BAUMANNII ISOLATES

Uğur Kayiş; Tammam Sipahi; Suzan Ökten; Fatma Kaynak Onurdağ

Makale detayları

INVESTIGATION OF DNA GYRASE RESISTANCE GENES IN ACINETOBACTER BAUMANNII ISOLATES

Yazarlar:

Uğur Kayiş | Tammam Sipahi | Suzan Ökten | Fatma Kaynak Onurdağ

Yayıncı:

Ankara University

Ana Başlıklar:

Farmasotik Mikrobiyoloji

Dergi:

Ankara Üniversitesi Eczacılık Fakültesi Dergisi Vol.49 Issue 1, pp.62-70

Belge Tipi:

Makale

Yayın Tarihi:

1/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001333050

pISSN:

1015-3918

Anahtar kelimeler:

Özet:

 Objective: It is known that bacteria resistant to antimicrobial agents can spread to the community in the hospital environment. Acinetobacter baumannii is a difficult-to-treat pathogen due to its antibiotic resistance genes. It is resistant to many antibiotics, including fluoroquinolones. By identifying mutations in DNA gyrase and topoisomerase IV genes, quinolone resistance of A. baumanni is found. Our study, aimed to determine mutations in DNA gyrase resistance genes that cause quinolone resistance gyrA, gyrB, parC in A. baumannii isolates using the real-time PCR method. Material and Method: 73 A. baumannii resistant clinical isolates were used in the study. A. baumannii was amplified using primers for gyrA, parC, gyrB genes. Using HRM analysis, gyrA, gyrB, parC gene Real-Time PCR amplicons were grouped according to their DNA sequences. A. baumannii isolates were grouped as P1, P2, P3, GA1, GA2, GA3, GB1 and GB2 according to different HRM profiles, DNA sequence analysis of 2 different Real-Time PCR products from each group was performed. Result and Discussion: Mutations were detected in GA1, GA2, GA3 genotypes. No mutations were observed in the P1, P2, P3 and, GB1, GB2 genotypes.

 Objective: It is known that bacteria resistant to antimicrobial agents can spread to the community in the hospital environment. Acinetobacter baumannii is a difficult-to-treat pathogen due to its antibiotic resistance genes. It is resistant to many antibiotics, including fluoroquinolones. By identifying mutations in DNA gyrase and topoisomerase IV genes, quinolone resistance of A. baumanni is found. Our study, aimed to determine mutations in DNA gyrase resistance genes that cause quinolone resistance gyrA, gyrB, parC in A. baumannii isolates using the real-time PCR method. Material and Method: 73 A. baumannii resistant clinical isolates were used in the study. A. baumannii was amplified using primers for gyrA, parC, gyrB genes. Using HRM analysis, gyrA, gyrB, parC gene Real-Time PCR amplicons were grouped according to their DNA sequences. A. baumannii isolates were grouped as P1, P2, P3, GA1, GA2, GA3, GB1 and GB2 according to different HRM profiles, DNA sequence analysis of 2 different Real-Time PCR products from each group was performed. Result and Discussion: Mutations were detected in GA1, GA2, GA3 genotypes. No mutations were observed in the P1, P2, P3 and, GB1, GB2 genotypes.

 Objective: It is known that bacteria resistant to antimicrobial agents can spread to the community in the hospital environment. Acinetobacter baumannii is a difficult-to-treat pathogen due to its antibiotic resistance genes. It is resistant to many antibiotics, including fluoroquinolones. By identifying mutations in DNA gyrase and topoisomerase IV genes, quinolone resistance of A. baumanni is found. Our study, aimed to determine mutations in DNA gyrase resistance genes that cause quinolone resistance gyrA, gyrB, parC in A. baumannii isolates using the real-time PCR method. Material and Method: 73 A. baumannii resistant clinical isolates were used in the study. A. baumannii was amplified using primers for gyrA, parC, gyrB genes. Using HRM analysis, gyrA, gyrB, parC gene Real-Time PCR amplicons were grouped according to their DNA sequences. A. baumannii isolates were grouped as P1, P2, P3, GA1, GA2, GA3, GB1 and GB2 according to different HRM profiles, DNA sequence analysis of 2 different Real-Time PCR products from each group was performed. Result and Discussion: Mutations were detected in GA1, GA2, GA3 genotypes. No mutations were observed in the P1, P2, P3 and, GB1, GB2 genotypes.

 Amaç: Hastane ortamındaki antimikrobiyal ajanlara dirençli bakterilerin topluma yayılabileceği bilinmektedir. Acinetobacter baumannii, antibiyotik direnç genleri sebebiyle tedavisi oldukça zor olan bir mikroorganizmadır. Florokinolonlar da dahil olmak üzere birçok antibiyotiğe dirençlidir. A. baumanninin kinolon direnci DNA giraz ve topoizomeraz IV genlerindeki mutasyonlarının belirlenmesiyle tespit edilir. Çalışmamızda Real Time PCR yöntemi kullanılarak, A. baumannii izolatlarında kinolon direncine sebep olan gyrA, gyrB, parC DNA giraz direnç genlerine ait mutasyonların belirlenmesi amaçlanmıştır. Gereç ve Yöntem: Çalışmada 73 adet A. baumannii dirençli klinik izolatı kullanıldı. A. baumannii gyrA, parC, gyrB genlerine ait primerler kullanılarak amplifiye edildi. HRM analizi kullanarak gyrA, gyrB, parC geni Real-Tıme PCR amplikonları DNA sekanslarına göre gruplara ayrıldı. A. baumannii izolatları farklı HRM profillerine göre P1, P2, P3, GA1, GA2, GA3, GB1 ve GB2 olarak gruplandırıldı ve her gruptan 2 farklı Real-Tıme PCR ürününün DNA dizi analizi yapıldı. Sonuç ve Tartışma: GA1, GA2, GA3 genotipinde mutasyonu tespit edildi. P1, P2, P3 ve GB1, GB2 genotiplerinde mutasyon gözlemlenmedi.

Makale detayları

INVESTIGATION OF DNA GYRASE RESISTANCE GENES IN ACINETOBACTER BAUMANNII ISOLATES

Yazarlar:

Uğur Kayiş | Tammam Sipahi | Suzan Ökten | Fatma Kaynak Onurdağ

Yayıncı:

Ankara University

Ana Başlıklar:

Farmasotik Mikrobiyoloji

Dergi:

Ankara Üniversitesi Eczacılık Fakültesi Dergisi Vol.49 Issue 1, pp.62-70

Belge Tipi:

Makale

Yayın Tarihi:

1/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001333050

pISSN:

1015-3918

Acinetobacter baumannii

DNA gyrase

HRM

RT-PCRAcinetobacter baumannii

DNA giraz

HRM

RT-PCR

Sayı

Makale detayları

Yazarlar: Uğur Kayiş | Tammam Sipahi | Suzan Ökten | Fatma Kaynak Onurdağ

Yayıncı: Ankara University

Ana Başlıklar: Farmasotik Mikrobiyoloji

Dergi: Ankara Üniversitesi Eczacılık Fakültesi Dergisi Vol.49 Issue 1, pp.62-70

Belge Tipi: Makale

Yayın Tarihi: 1/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001333050

pISSN: 1015-3918

Sayı

Yazarlar:

Uğur Kayiş | Tammam Sipahi | Suzan Ökten | Fatma Kaynak Onurdağ

Yayıncı:

Ankara University

Ana Başlıklar:

Farmasotik Mikrobiyoloji

Dergi:

Ankara Üniversitesi Eczacılık Fakültesi Dergisi Vol.49 Issue 1, pp.62-70

Belge Tipi:

Makale

Yayın Tarihi:

1/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001333050

pISSN:

1015-3918