An efficient multiplex PCR assay for early detection of Agrobacterium tumefaciens in transgenic plant materials

Li YANG; Changchun WANG; Lihuan WANG; Changjie XU; Kunsong CHEN

Makale detayları

An efficient multiplex PCR assay for early detection of Agrobacterium tumefaciens in transgenic plant materials

Yazarlar:

Li YANG | Changchun WANG | Lihuan WANG | Changjie XU | Kunsong CHEN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Agriculture and Forestry 2013 Vol.37 Issue 2, pp.157-162

Belge Tipi:

Makale

Yayın Tarihi:

1/2013

Belge Dili:

Türkçe

Document ID (Org No.):

2000807703

Sıklık:

İki Aylık

eISSN:

1303-6173

pISSN:

1300-011X

Anahtar kelimeler:

Özet:

 

The multiplex polymerase chain reaction (MPCR) is a variant of conventional PCR in which 2 or more target sequences are amplified by more than 1 pair of primers in a single reaction mixture. In this study, an efficient MPCR assay was established and optimized for the quick detection of Agrobacterium-derived plant transformants. The neomycin phosphotransferase II gene fragment (nptII; target gene) and the chromosomal virulence gene ChvA fragment of Agrobacterium tumefaciens (external reference gene) were amplified simultaneously with 2 pairs of corresponding primers. The MPCR results showed that 9 out of 10 kanamycin-resistant lines tested were transformed with the foreign genes. This optimized MPCR assay, rapid, sensitive, and reliable, can facilitate the identification of Agrobacterium-derived plant transformants at the early stage of selection.

 

The multiplex polymerase chain reaction (MPCR) is a variant of conventional PCR in which 2 or more target sequences are amplified by more than 1 pair of primers in a single reaction mixture. In this study, an efficient MPCR assay was established and optimized for the quick detection of Agrobacterium-derived plant transformants. The neomycin phosphotransferase II gene fragment (nptII; target gene) and the chromosomal virulence gene ChvA fragment of Agrobacterium tumefaciens (external reference gene) were amplified simultaneously with 2 pairs of corresponding primers. The MPCR results showed that 9 out of 10 kanamycin-resistant lines tested were transformed with the foreign genes. This optimized MPCR assay, rapid, sensitive, and reliable, can facilitate the identification of Agrobacterium-derived plant transformants at the early stage of selection.

Makale detayları

An efficient multiplex PCR assay for early detection of Agrobacterium tumefaciens in transgenic plant materials

Yazarlar:

Li YANG | Changchun WANG | Lihuan WANG | Changjie XU | Kunsong CHEN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Agriculture and Forestry 2013 Vol.37 Issue 2, pp.157-162

Belge Tipi:

Makale

Yayın Tarihi:

1/2013

Belge Dili:

Türkçe

Document ID (Org No.):

2000807703

Sıklık:

İki Aylık

eISSN:

1303-6173

pISSN:

1300-011X

Key words: Agrobacterium tumefaciens

multiplex polymerase chain reaction

transgenic plant detection

Sayı

Makale detayları

An efficient multiplex PCR assay for early detection of Agrobacterium tumefaciens in transgenic plant materials

Yazarlar: Li YANG | Changchun WANG | Lihuan WANG | Changjie XU | Kunsong CHEN

Yayıncı: TUBITAK

Dergi: Turkish Journal of Agriculture and Forestry 2013 Vol.37 Issue 2, pp.157-162

Belge Tipi: Makale

Yayın Tarihi: 1/2013

Belge Dili: Türkçe

Document ID (Org No.): 2000807703

Sıklık: İki Aylık

eISSN: 1303-6173

pISSN: 1300-011X

Key words: Agrobacterium tumefaciens

multiplex polymerase chain reaction

transgenic plant detection

Sayı

Yazarlar:

Li YANG | Changchun WANG | Lihuan WANG | Changjie XU | Kunsong CHEN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Agriculture and Forestry 2013 Vol.37 Issue 2, pp.157-162

Belge Tipi:

Makale

Yayın Tarihi:

1/2013

Belge Dili:

Türkçe

Document ID (Org No.):

2000807703

Sıklık:

İki Aylık

eISSN:

1303-6173

pISSN:

1300-011X