In vitro antiproliferative/cytotoxic activity of 2,3'-biindole against various cancer cell lines

Salih ÖKTEN; Ramazan ERENLER; Tuğba KUL KÖPRÜLÜ; Şaban TEKİN

Makale detayları

In vitro antiproliferative/cytotoxic activity of 2,3'-biindole against various cancer cell lines

Yazarlar:

Salih ÖKTEN | Ramazan ERENLER | Tuğba KUL KÖPRÜLÜ | Şaban TEKİN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2015 Vol.39 Issue 1, pp.15-22

Belge Tipi:

Makale

Yayın Tarihi:

3/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000812010

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Anahtar kelimeler:

Özet:

 

2,3'-Biindole (2) was synthesized via bromination of indole (1) with molecular bromine and underwent simultaneous dimerization. Antiproliferative and cytotoxic activity of 2 was investigated in vitro on C6 (rat brain tumor), HeLa (human cervix carcinoma), and HT29 (human colon carcinoma) cells lines by using BrdU cell proliferation ELISA and lactate dehydrogenase (LDH) assays. In contrast to 5-fluorouracil (5-FU), 2,3'-biindole (2) significantly inhibited proliferation of HeLa and HT29 cell lines. According to LDH assay, the cytotoxicity of compound 2 was low on HT29 cell lines and high on HeLa and C6 cell lines. Moreover, 2 did not cause any DNA laddering on the DNA of tested cells; therefore, it is suggested that the mechanism of action of this compound may not involve apoptosis. In addition, 2 inhibited relaxation of supercoiled plasmid DNA by topoisomerase activity. Results of the present study indicates that biindole (2) may have promising anticancer and antitopoisomerase potential with an unknown mechanism of action.

 

2,3'-Biindole (2) was synthesized via bromination of indole (1) with molecular bromine and underwent simultaneous dimerization. Antiproliferative and cytotoxic activity of 2 was investigated in vitro on C6 (rat brain tumor), HeLa (human cervix carcinoma), and HT29 (human colon carcinoma) cells lines by using BrdU cell proliferation ELISA and lactate dehydrogenase (LDH) assays. In contrast to 5-fluorouracil (5-FU), 2,3'-biindole (2) significantly inhibited proliferation of HeLa and HT29 cell lines. According to LDH assay, the cytotoxicity of compound 2 was low on HT29 cell lines and high on HeLa and C6 cell lines. Moreover, 2 did not cause any DNA laddering on the DNA of tested cells; therefore, it is suggested that the mechanism of action of this compound may not involve apoptosis. In addition, 2 inhibited relaxation of supercoiled plasmid DNA by topoisomerase activity. Results of the present study indicates that biindole (2) may have promising anticancer and antitopoisomerase potential with an unknown mechanism of action.

Makale detayları

In vitro antiproliferative/cytotoxic activity of 2,3'-biindole against various cancer cell lines

Yazarlar:

Salih ÖKTEN | Ramazan ERENLER | Tuğba KUL KÖPRÜLÜ | Şaban TEKİN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2015 Vol.39 Issue 1, pp.15-22

Belge Tipi:

Makale

Yayın Tarihi:

3/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000812010

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

2

3'-Biindole

anticancer

cytotoxicity

apoptosis

antitopoisomerase

C6 cell line

HeLa cell line

HT29 cell line

Sayı

Makale detayları

Yazarlar: Salih ÖKTEN | Ramazan ERENLER | Tuğba KUL KÖPRÜLÜ | Şaban TEKİN

Yayıncı: TUBITAK

Dergi: Turkish Journal of Biology 2015 Vol.39 Issue 1, pp.15-22

Belge Tipi: Makale

Yayın Tarihi: 3/2015

Belge Dili: Türkçe

Document ID (Org No.): 2000812010

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

Sayı

Yazarlar:

Salih ÖKTEN | Ramazan ERENLER | Tuğba KUL KÖPRÜLÜ | Şaban TEKİN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2015 Vol.39 Issue 1, pp.15-22

Belge Tipi:

Makale

Yayın Tarihi:

3/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000812010

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152