The influence of toluidine blue staining on decision-making for the selection of biopsy sites in oral disorders

Ceyda Gürhan; Betül İlhan; Barış Oğuz Gürses; Gaye Bölükbaşı; Pelin Güneri

Makale detayları

The influence of toluidine blue staining on decision-making for the selection of biopsy sites in oral disorders

Yazarlar:

Ceyda Gürhan | Betül İlhan | Barış Oğuz Gürses | Gaye Bölükbaşı | Pelin Güneri

Yayıncı:

Istanbul University

Ana Başlıklar:

Dentistry (Other)

Dergi:

European Oral Research Vol.59 Issue 2, pp.144-149

Belge Tipi:

Makale

Yayın Tarihi:

5/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001352464

pISSN:

2630-6158

Anahtar kelimeler:

Özet:

 Purpose: The aim of this study is to evaluate the influence of toluidine blue TB staining on decision-making processes for the identification of biopsy sites in oral lesions. Material-Methods: Original and TB-stained images of oral mucosal lesions that required histopathological examination were accessed through archives. Twenty dental specialists were asked to select and mark their choice of biopsy sites on both original and TB-stained images using a standard circle selection tool of software. The X and Y coordinates of the center points of selections were calculated by a custom made image processing algorithm in Matlab. In order to assess the dispersity of the selected centers from the mean center, the distances of the selected points from this average point were calculated using Euclidean distance. Data was analysed using Wilcoxon test. Results: The study involved original and TB-stained images of 5 suspicious lesions. The histological diagnoses of oral lesions were reported as bening for 3 cases and malignant for 2 cases. 20 dental specialists marked their choice of biopsy site on a total of 200 images. The results revealed that the biopsy selections varied significantly between original and TB-stained images in certain cases. In 60 of cases, specialists showed statistically significant agreement in biopsy site selection on TB-stained images compared to original images p 0.05. Conclusion: While the study did not evaluate the accuracy of site selection against a gold standard, the observed consensus among specialists suggests the potential utility of TB staining in enhancing the consistency and objectivity of biopsy site selection for suspicious oral lesions.

 Purpose: The aim of this study is to evaluate the influence of toluidine blue TB staining on decision-making processes for the identification of biopsy sites in oral lesions. Material-Methods: Original and TB-stained images of oral mucosal lesions that required histopathological examination were accessed through archives. Twenty dental specialists were asked to select and mark their choice of biopsy sites on both original and TB-stained images using a standard circle selection tool of software. The X and Y coordinates of the center points of selections were calculated by a custom made image processing algorithm in Matlab. In order to assess the dispersity of the selected centers from the mean center, the distances of the selected points from this average point were calculated using Euclidean distance. Data was analysed using Wilcoxon test. Results: The study involved original and TB-stained images of 5 suspicious lesions. The histological diagnoses of oral lesions were reported as bening for 3 cases and malignant for 2 cases. 20 dental specialists marked their choice of biopsy site on a total of 200 images. The results revealed that the biopsy selections varied significantly between original and TB-stained images in certain cases. In 60 of cases, specialists showed statistically significant agreement in biopsy site selection on TB-stained images compared to original images p 0.05. Conclusion: While the study did not evaluate the accuracy of site selection against a gold standard, the observed consensus among specialists suggests the potential utility of TB staining in enhancing the consistency and objectivity of biopsy site selection for suspicious oral lesions.

 Purpose: The aim of this study is to evaluate the influence of toluidine blue TB staining on decision-making processes for the identification of biopsy sites in oral lesions. Material-Methods: Original and TB-stained images of oral mucosal lesions that required histopathological examination were accessed through archives. Twenty dental specialists were asked to select and mark their choice of biopsy sites on both original and TB-stained images using a standard circle selection tool of software. The X and Y coordinates of the center points of selections were calculated by a custom made image processing algorithm in Matlab. In order to assess the dispersity of the selected centers from the mean center, the distances of the selected points from this average point were calculated using Euclidean distance. Data was analysed using Wilcoxon test. Results: The study involved original and TB-stained images of 5 suspicious lesions. The histological diagnoses of oral lesions were reported as bening for 3 cases and malignant for 2 cases. 20 dental specialists marked their choice of biopsy site on a total of 200 images. The results revealed that the biopsy selections varied significantly between original and TB-stained images in certain cases. In 60 of cases, specialists showed statistically significant agreement in biopsy site selection on TB-stained images compared to original images p 0.05. Conclusion: While the study did not evaluate the accuracy of site selection against a gold standard, the observed consensus among specialists suggests the potential utility of TB staining in enhancing the consistency and objectivity of biopsy site selection for suspicious oral lesions.

 Purpose: The aim of this study is to evaluate the influence of toluidine blue TB staining on decision-making processes for the identification of biopsy sites in oral lesions. Material-Methods: Original and TB-stained images of oral mucosal lesions that required histopathological examination were accessed through archives. Twenty dental specialists were asked to select and mark their choice of biopsy sites on both original and TB-stained images using a standard circle selection tool of software. The X and Y coordinates of the center points of selections were calculated by a custom made image processing algorithm in Matlab. In order to assess the dispersity of the selected centers from the mean center, the distances of the selected points from this average point were calculated using Euclidean distance. Data was analysed using Wilcoxon test. Results: The study involved original and TB-stained images of 5 suspicious lesions. The histological diagnoses of oral lesions were reported as bening for 3 cases and malignant for 2 cases. 20 dental specialists marked their choice of biopsy site on a total of 200 images. The results revealed that the biopsy selections varied significantly between original and TB-stained images in certain cases. In 60 of cases, specialists showed statistically significant agreement in biopsy site selection on TB-stained images compared to original images p 0.05. Conclusion: While the study did not evaluate the accuracy of site selection against a gold standard, the observed consensus among specialists suggests the potential utility of TB staining in enhancing the consistency and objectivity of biopsy site selection for suspicious oral lesions.

Makale detayları

The influence of toluidine blue staining on decision-making for the selection of biopsy sites in oral disorders

Yazarlar:

Ceyda Gürhan | Betül İlhan | Barış Oğuz Gürses | Gaye Bölükbaşı | Pelin Güneri

Yayıncı:

Istanbul University

Ana Başlıklar:

Dentistry (Other)

Dergi:

European Oral Research Vol.59 Issue 2, pp.144-149

Belge Tipi:

Makale

Yayın Tarihi:

5/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001352464

pISSN:

2630-6158

Oral mucosal lesion

tissue biopsy

biopsy site selection

vital staining

toluidine blue

Sayı

Makale detayları

The influence of toluidine blue staining on decision-making for the selection of biopsy sites in oral disorders

Yazarlar: Ceyda Gürhan | Betül İlhan | Barış Oğuz Gürses | Gaye Bölükbaşı | Pelin Güneri

Yayıncı: Istanbul University

Ana Başlıklar: Dentistry (Other)

Dergi: European Oral Research Vol.59 Issue 2, pp.144-149

Belge Tipi: Makale

Yayın Tarihi: 5/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001352464

pISSN: 2630-6158

Oral mucosal lesion

tissue biopsy

biopsy site selection

vital staining

toluidine blue

Sayı

Yazarlar:

Ceyda Gürhan | Betül İlhan | Barış Oğuz Gürses | Gaye Bölükbaşı | Pelin Güneri

Yayıncı:

Istanbul University

Ana Başlıklar:

Dentistry (Other)

Dergi:

European Oral Research Vol.59 Issue 2, pp.144-149

Belge Tipi:

Makale

Yayın Tarihi:

5/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001352464

pISSN:

2630-6158