Analysis of Functional Domains on Glutamate  Synthase

Barbaros NALBANTOĞLU

Makale detayları

Analysis of Functional Domains on Glutamate Synthase

Yazarlar:

Barbaros NALBANTOĞLU

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2000 Vol.24 Issue 2, pp.197-214

Belge Tipi:

Makale

Yayın Tarihi:

1/2000

Belge Dili:

Türkçe

Document ID (Org No.):

2000814057

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Anahtar kelimeler:

Özet:

 

Glutamate synthases (GOGAT) were analyzed to identify the functional binding domains of  the substrate (glutamine) and cofactors (FMN, NAD(P)H, FAD, [3Fe-4S]1+,0 and [4Fe-4S]2+,1+  clusters and ferredoxin) on this enzyme. The published amino acid sequences of six different NAD(P)H-dependent  GOGATs (NAD(P)H-GOGAT) and ten different ferredoxin-dependent GOGATs (Fd-GOGAT)  were used for this analysis. The amino acid sequences of these sixteen GOGATs were compared with  the amino acid sequences of aminotransferases for glutamine, flavoproteins for FMN, flavoproteins  and pyridine-nucleotide-dependent enzymes for FAD and NAD(P)H, iron-sulfur proteins for [3Fe-4S]  1+,0 and [4Fe-4S]2+,1+ clusters and ferredoxin-dependent enzymes for ferredoxin. It was  determined that Fd-GOGAT has one domain each for glutamine, FMN and [3Fe-4S]1+,0 cluster and two  domains each for FAD and ferredoxin; the NADPH-GOGAT a subunit has the same domains as Fd-GOGAT  except for the ferredoxin domains, and b subunit has one domain each for NADPH and FAD  and two domains for two [4Fe-4S]2+,1+ clusters; NADH-GOGAT has the same domains as NADPH-GOGAT.

 

Glutamate synthases (GOGAT) were analyzed to identify the functional binding domains of  the substrate (glutamine) and cofactors (FMN, NAD(P)H, FAD, [3Fe-4S]1+,0 and [4Fe-4S]2+,1+  clusters and ferredoxin) on this enzyme. The published amino acid sequences of six different NAD(P)H-dependent  GOGATs (NAD(P)H-GOGAT) and ten different ferredoxin-dependent GOGATs (Fd-GOGAT)  were used for this analysis. The amino acid sequences of these sixteen GOGATs were compared with  the amino acid sequences of aminotransferases for glutamine, flavoproteins for FMN, flavoproteins  and pyridine-nucleotide-dependent enzymes for FAD and NAD(P)H, iron-sulfur proteins for [3Fe-4S]  1+,0 and [4Fe-4S]2+,1+ clusters and ferredoxin-dependent enzymes for ferredoxin. It was  determined that Fd-GOGAT has one domain each for glutamine, FMN and [3Fe-4S]1+,0 cluster and two  domains each for FAD and ferredoxin; the NADPH-GOGAT a subunit has the same domains as Fd-GOGAT  except for the ferredoxin domains, and b subunit has one domain each for NADPH and FAD  and two domains for two [4Fe-4S]2+,1+ clusters; NADH-GOGAT has the same domains as NADPH-GOGAT.

Makale detayları

Analysis of Functional Domains on Glutamate Synthase

Yazarlar:

Barbaros NALBANTOĞLU

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2000 Vol.24 Issue 2, pp.197-214

Belge Tipi:

Makale

Yayın Tarihi:

1/2000

Belge Dili:

Türkçe

Document ID (Org No.):

2000814057

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Glutamate synthase

glutamine

FMN

FAD

NAD(P)H

iron-sulfur cluster

ferredoxin

binding domain.

Sayı

Makale detayları

Yazarlar: Barbaros NALBANTOĞLU

Yayıncı: TUBITAK

Dergi: Turkish Journal of Biology 2000 Vol.24 Issue 2, pp.197-214

Belge Tipi: Makale

Yayın Tarihi: 1/2000

Belge Dili: Türkçe

Document ID (Org No.): 2000814057

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

Sayı

Yazarlar:

Barbaros NALBANTOĞLU

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2000 Vol.24 Issue 2, pp.197-214

Belge Tipi:

Makale

Yayın Tarihi:

1/2000

Belge Dili:

Türkçe

Document ID (Org No.):

2000814057

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152