Comparison of Bone Morphogenetic Protein -2 Secretion Capacity of Myoma, Myometrium and Endometrium

Yeliz Aykanat; Fulya Gokdagli Sagir; Lebriz Hale Aktün Tamer; Şükriye Leyla Altuntaş; Betül Dündar; Türkan Yiğitbaşı

Makale detayları

Comparison of Bone Morphogenetic Protein -2 Secretion Capacity of Myoma, Myometrium and Endometrium

Yazarlar:

Yeliz Aykanat | Fulya Gokdagli Sagir | Lebriz Hale Aktün Tamer | Şükriye Leyla Altuntaş | Betül Dündar | Türkan Yiğitbaşı

Yayıncı:

Van Yuzuncu Yıl University

Ana Başlıklar:

Cerrahi (Diğer)

Dergi:

Van Sağlık Bilimleri Dergisi Vol.18 Issue 1, pp.14-19

Belge Tipi:

Makale

Yayın Tarihi:

4/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001346810

pISSN:

2667-5072

Anahtar kelimeler:

Özet:

 Introduction: Bone morphogenetic protein-2 BMP-2 is a member of the transforming growth factor β3 TGF-β3 family and plays a crucial role in decidualization and implantation. Myomas can adversely affect endometrial receptivity and embryo implantation. Recent studies have highlighted the importance of understanding the molecular mechanisms underlying endometrial receptivity. The aim of this study is to determine BMP-2 secretion capacity of intramural, subserous, submucous myomas and their effects on endometrial tissue BMP-2 levels. Material- methods: Ninety-seven women who underwent hysterectomy or myomectomy because of symptomatic myomas were divided into three groups as submucous SMM, n26, intramural IMM, n34, and subserous SSM, n37. Myoma, myometrium and endometrial tissue samples were isolated and homogenized. BMP-2 concentrations of myoma, myometrium and endometrium tissue samples were evaluated with ELISA kits. Results: BMP-2 levels showed no significant difference between myoma samples of SMM, IMM, SSM p0,05. Within the groups, endometrium of SMM have the lowest BMP-2 levels 2417720 pgml, p value 0,05. BMP-2 levels in endometrium of SSM and IMM groups were higher than myoma and myometrium of SSM and IMM groups. SMM group did not show any difference in BMP-2 levels in endometrium, myometrium and myoma. Conclusion: Subserous, submucous and intramural myomas show different effect on BMP-2 expression in endometrium, myometrium and myoma. SSM and IMM have no impact on BMP-2 expression of endometrium. But SMM alter release of BMP-2 in endometrium. Decreased BMP-2 levels may be the responsible mechanism for impared implantation and decreased pregnancy rates in this group of patients. Further investigations may lead to the development of novel therapeutics aimed at restoring BMP-2 levels.

 Introduction: Bone morphogenetic protein-2 BMP-2 is a member of the transforming growth factor β3 TGF-β3 family and plays a crucial role in decidualization and implantation. Myomas can adversely affect endometrial receptivity and embryo implantation. Recent studies have highlighted the importance of understanding the molecular mechanisms underlying endometrial receptivity. The aim of this study is to determine BMP-2 secretion capacity of intramural, subserous, submucous myomas and their effects on endometrial tissue BMP-2 levels. Material- methods: Ninety-seven women who underwent hysterectomy or myomectomy because of symptomatic myomas were divided into three groups as submucous SMM, n26, intramural IMM, n34, and subserous SSM, n37. Myoma, myometrium and endometrial tissue samples were isolated and homogenized. BMP-2 concentrations of myoma, myometrium and endometrium tissue samples were evaluated with ELISA kits. Results: BMP-2 levels showed no significant difference between myoma samples of SMM, IMM, SSM p0,05. Within the groups, endometrium of SMM have the lowest BMP-2 levels 2417720 pgml, p value 0,05. BMP-2 levels in endometrium of SSM and IMM groups were higher than myoma and myometrium of SSM and IMM groups. SMM group did not show any difference in BMP-2 levels in endometrium, myometrium and myoma. Conclusion: Subserous, submucous and intramural myomas show different effect on BMP-2 expression in endometrium, myometrium and myoma. SSM and IMM have no impact on BMP-2 expression of endometrium. But SMM alter release of BMP-2 in endometrium. Decreased BMP-2 levels may be the responsible mechanism for impared implantation and decreased pregnancy rates in this group of patients. Further investigations may lead to the development of novel therapeutics aimed at restoring BMP-2 levels.

 Introduction: Bone morphogenetic protein-2 BMP-2 is a member of the transforming growth factor β3 TGF-β3 family and plays a crucial role in decidualization and implantation. Myomas can adversely affect endometrial receptivity and embryo implantation. Recent studies have highlighted the importance of understanding the molecular mechanisms underlying endometrial receptivity. The aim of this study is to determine BMP-2 secretion capacity of intramural, subserous, submucous myomas and their effects on endometrial tissue BMP-2 levels. Material- methods: Ninety-seven women who underwent hysterectomy or myomectomy because of symptomatic myomas were divided into three groups as submucous SMM, n26, intramural IMM, n34, and subserous SSM, n37. Myoma, myometrium and endometrial tissue samples were isolated and homogenized. BMP-2 concentrations of myoma, myometrium and endometrium tissue samples were evaluated with ELISA kits. Results: BMP-2 levels showed no significant difference between myoma samples of SMM, IMM, SSM p0,05. Within the groups, endometrium of SMM have the lowest BMP-2 levels 2417720 pgml, p value 0,05. BMP-2 levels in endometrium of SSM and IMM groups were higher than myoma and myometrium of SSM and IMM groups. SMM group did not show any difference in BMP-2 levels in endometrium, myometrium and myoma. Conclusion: Subserous, submucous and intramural myomas show different effect on BMP-2 expression in endometrium, myometrium and myoma. SSM and IMM have no impact on BMP-2 expression of endometrium. But SMM alter release of BMP-2 in endometrium. Decreased BMP-2 levels may be the responsible mechanism for impared implantation and decreased pregnancy rates in this group of patients. Further investigations may lead to the development of novel therapeutics aimed at restoring BMP-2 levels.

 Giriş: Bone morfogenetik protein-2 BMP-2, transforming growth faktörü β3 TGF-β3 ailesinin bir üyesi olup, desidualizasyon ve implantasyonda kritik bir rol oynar. Miyomlar, endometrial reseptivite ve embriyo implantasyonunu olumsuz etkileyebilir. Son çalışmalar, endometrial reseptivitenin temelinde yatan moleküler mekanizmaların anlaşılmasının önemini vurgulamıştır. Bu çalışmanın amacı, intramural, subseröz, submuköz miyomların BMP-2 salgı kapasitesini ve endometrial dokuda BMP-2 seviyelerine olan etkilerini belirlemektir. Materyal ve Metodlar: Semptomatik miyomlar nedeniyle histerektomi veya miyomektomi geçiren 97 kadın, submuköz SMM, n26, intramural IMM, n34 ve subseröz SSM, n37 olmak üzere üç gruba ayrıldı. Miyom, miyometrium ve endometrial doku örnekleri izole edilip homojenize edildi. Miyom, miyometrium ve endometrium doku örneklerinin BMP-2 konsantrasyonları ELISA kitleri kullanılarak değerlendirildi. Sonuçlar: BMP-2 seviyeleri, SMM, IMM, ve SSM gruplarının miyom örnekleri arasında anlamlı bir farklılık göstermedi p0,05. Ancak, bu gruplar içinde, SMM grubunun endometriumunda en düşük BMP-2 seviyelerini saptandı 2417720 pgml, p değeri 0,05. SSM ve IMM gruplarının endometriumundaki BMP-2 seviyeleri, aynı gruplardaki miyom ve miyometrium örneklerinden daha yüksekti. SMM grubu, endometrium, miyometrium ve miyom dokuları arasında BMP-2 seviyelerinde farklılık göstermedi. Sonuç: Subseröz, submüköz ve intramural miyomlar endometriyum, miyometrium ve miyomdaki BMP-2 ekspresyonu üzerinde farklı etkiler gösterir. Çalışmamızda SSM ve IMMların endometriyumun BMP-2 ekspresyonu üzerine etkisi gösterilememiştir. SMMlar ise endometriumda BMP-2 salınımını değiştirmiştir. Azalmış BMP-2 seviyeleri, bu hasta grubunda daha sık karşımıza çıkan implantasyonun bozulması ve azalmış gebelik oranlarından sorumlu mekanizma olabilir. BMP-2 seviyelerinin artırılmasını sağlayan tedaviler, gelecek çalışmalar için araştırma konusu olabilir.

Makale detayları

Comparison of Bone Morphogenetic Protein -2 Secretion Capacity of Myoma, Myometrium and Endometrium

Yazarlar:

Yeliz Aykanat | Fulya Gokdagli Sagir | Lebriz Hale Aktün Tamer | Şükriye Leyla Altuntaş | Betül Dündar | Türkan Yiğitbaşı

Yayıncı:

Van Yuzuncu Yıl University

Ana Başlıklar:

Cerrahi (Diğer)

Dergi:

Van Sağlık Bilimleri Dergisi Vol.18 Issue 1, pp.14-19

Belge Tipi:

Makale

Yayın Tarihi:

4/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001346810

pISSN:

2667-5072

myoma uteri

Bone morphogenetic protein -2

infertilitymyom

infertilite

bone morfogenetik proteini-2

Sayı

Makale detayları

Comparison of Bone Morphogenetic Protein -2 Secretion Capacity of Myoma, Myometrium and Endometrium

Yazarlar: Yeliz Aykanat | Fulya Gokdagli Sagir | Lebriz Hale Aktün Tamer | Şükriye Leyla Altuntaş | Betül Dündar | Türkan Yiğitbaşı

Yayıncı: Van Yuzuncu Yıl University

Ana Başlıklar: Cerrahi (Diğer)

Dergi: Van Sağlık Bilimleri Dergisi Vol.18 Issue 1, pp.14-19

Belge Tipi: Makale

Yayın Tarihi: 4/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001346810

pISSN: 2667-5072

myoma uteri

Bone morphogenetic protein -2

infertilitymyom

infertilite

bone morfogenetik proteini-2

Sayı

Yazarlar:

Yeliz Aykanat | Fulya Gokdagli Sagir | Lebriz Hale Aktün Tamer | Şükriye Leyla Altuntaş | Betül Dündar | Türkan Yiğitbaşı

Yayıncı:

Van Yuzuncu Yıl University

Ana Başlıklar:

Cerrahi (Diğer)

Dergi:

Van Sağlık Bilimleri Dergisi Vol.18 Issue 1, pp.14-19

Belge Tipi:

Makale

Yayın Tarihi:

4/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001346810

pISSN:

2667-5072