Detection of Shiga-Toxin Producing E.coli (STEC), Enteropathogenic E.coli (EPEC) And Enterotoxigenic E.coli (ETEC) From Animals By M-PCR

Esra BÜYÜKCANGAZ; Mohammed KHİDER; Ayşin ŞEN; Murat CENGİZ; Songül SONAL

doi:https://doi.org/10.30782/jrvm.624890

Makale detayları

Detection of Shiga-Toxin Producing E.coli (STEC), Enteropathogenic E.coli (EPEC) And Enterotoxigenic E.coli (ETEC) From Animals By M-PCR

Yazarlar:

Esra BÜYÜKCANGAZ | Mohammed KHİDER | Ayşin ŞEN | Murat CENGİZ | Songül SONAL

Yayıncı:

Bursa Uludağ University

Ana Başlıklar:

Hayvancılık

Dergi:

Journal of Research in Veterinary Medicine 2019 Vol.38 Issue 2, pp.88-94

Belge Tipi:

Makale

Yayın Tarihi:

12/2019

Belge Dili:

İngilizce

Document ID (Org No.):

2000493995

Sıklık:

İki Yıllık

eISSN:

2667-6745

DOI:

https://doi.org/10.30782/jrvm.624890

Özet:

 


This research investigated the presence of virulence
genes encoding F41, K99, eae, Stx1, Stx2 and STa and the antimicrobial resistance of
animal Escherichia coli (E. coli) isolates. Clinical isolates (n:233) were
evaluated from faecal samples of cattle, sheep, goats, horses, cats and dogs collected
between the years of 2010 to 2015 from Turkey.
Enterohaemorrahic E.coli (EHEC) O157:H7
was detected by using cefixime tellurite sorbitol MacConkey agar (CT-SMAC) and
Wellcollex E. coli (Remel®). The Kirby-Bauer disc diffusion test was performed to detect the resistance pattern
of the isolates to ampicillin, Amoxycillin/clavulanic acid, enrofloxacin,
ceftiofur, trimethoprim/sulfamethoxazole and tetracycline. The results showed
that 40% of the ruminant isolates were identified as Shiga-toxin producing Escherichia coli (STEC).  Enterotoxigenic E. coli (ETEC) was detected
in samples from cattle (0.9%) and sheep (12%). Enteropathogenic E.coli (EPEC) was detected in samples
from cattle (0.9%) and dogs (11.4%). EHEC O157:H7 was not detected any
of the isolates. Among all E. coli isolates that carried at least one
virulence gene, 8 (19%) were resistant to more than three antimicrobials, 7 (16.7%)
were resistant to at least one antimicrobial and 27 (64.3%) were susceptible to
all antimicrobials.


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Makale detayları

Detection of Shiga-Toxin Producing E.coli (STEC), Enteropathogenic E.coli (EPEC) And Enterotoxigenic E.coli (ETEC) From Animals By M-PCR

Yazarlar: Esra BÜYÜKCANGAZ | Mohammed KHİDER | Ayşin ŞEN | Murat CENGİZ | Songül SONAL

Yayıncı: Bursa Uludağ University

Ana Başlıklar: Hayvancılık

Dergi: Journal of Research in Veterinary Medicine 2019 Vol.38 Issue 2, pp.88-94

Belge Tipi: Makale

Yayın Tarihi: 12/2019

Belge Dili: İngilizce

Document ID (Org No.): 2000493995

Sıklık: İki Yıllık

eISSN: 2667-6745

DOI: https://doi.org/10.30782/jrvm.624890

Sayı

Yazarlar:

Esra BÜYÜKCANGAZ | Mohammed KHİDER | Ayşin ŞEN | Murat CENGİZ | Songül SONAL

Yayıncı:

Bursa Uludağ University

Ana Başlıklar:

Hayvancılık

Dergi:

Journal of Research in Veterinary Medicine 2019 Vol.38 Issue 2, pp.88-94

Belge Tipi:

Makale

Yayın Tarihi:

12/2019

Belge Dili:

İngilizce

Document ID (Org No.):

2000493995

Sıklık:

İki Yıllık

eISSN:

2667-6745

DOI:

https://doi.org/10.30782/jrvm.624890