The antifungal effect of propolis extract against cotton wild disease (Verticillium dahliae Kleb.)

Yeşim Kara; Oktay Erdoğan

Makale detayları

The antifungal effect of propolis extract against cotton wild disease (Verticillium dahliae Kleb.)

Yazarlar:

Yeşim Kara | Oktay Erdoğan

Yayıncı:

İzzet KARA

Ana Başlıklar:

Structural Biology

Dergi:

International Journal of Secondary Metabolite Vol.10 Issue 2, pp.257-268

Belge Tipi:

Makale

Yayın Tarihi:

6/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001289448

pISSN:

2148-6905

Anahtar kelimeler:

Özet:

 The aim of this study is to investigate the antifungal activity of propolis against V. dahliae Kleb. under both in vitro and in vivo conditions. Firstly, the inhibitory effect of the propolis on mycelial growth in Potato Dextrose Agar PDA media containing its ethanol extract PE at various concentrations 0.003, 0.06, 0.125, 0.25, 0.5, and 1 ppmmL was investigated under in vitro conditions. Then to assess the effect of PE on non-defoliating PHCVd3 isolate and defoliating PHCVd47 isolate pathotypes of V. dahliae, the varieties Giza 45 resistant, Carmen tolerant, and Acala SJ2 susceptible treated with PEE 1 ppmmL were observed in the plant growth chamber up to the 4-6 leaf stage. The whole in vitro experiments were carried out with three replicates, and the studies in vivo experiment were with five replicates depending on a completely randomized parcels design. The most effective dose of PEE with1 ppmmL dose resulted in 75.2 suppression against the PHCVd3 isolate, while the effect of the same dose against the PHCVd47 isolate was 74.4. The lowest disease severity index DSI values against PHCVd3 and PHCVd47 isolates in cotton cultivars treated with PE were 1.34 and 1.64 in the Giza 45, respectively, and the highest DSI values were 3.80 and 3.90 in the Acala SJ2 cultivar in vivo experiment, respectively. The findings indicate that PE treatment has a promising effect against cotton wilt disease that could be combined with known plant protection strategies.

 The aim of this study is to investigate the antifungal activity of propolis against V. dahliae Kleb. under both in vitro and in vivo conditions. Firstly, the inhibitory effect of the propolis on mycelial growth in Potato Dextrose Agar PDA media containing its ethanol extract PE at various concentrations 0.003, 0.06, 0.125, 0.25, 0.5, and 1 ppmmL was investigated under in vitro conditions. Then to assess the effect of PE on non-defoliating PHCVd3 isolate and defoliating PHCVd47 isolate pathotypes of V. dahliae, the varieties Giza 45 resistant, Carmen tolerant, and Acala SJ2 susceptible treated with PEE 1 ppmmL were observed in the plant growth chamber up to the 4-6 leaf stage. The whole in vitro experiments were carried out with three replicates, and the studies in vivo experiment were with five replicates depending on a completely randomized parcels design. The most effective dose of PEE with1 ppmmL dose resulted in 75.2 suppression against the PHCVd3 isolate, while the effect of the same dose against the PHCVd47 isolate was 74.4. The lowest disease severity index DSI values against PHCVd3 and PHCVd47 isolates in cotton cultivars treated with PE were 1.34 and 1.64 in the Giza 45, respectively, and the highest DSI values were 3.80 and 3.90 in the Acala SJ2 cultivar in vivo experiment, respectively. The findings indicate that PE treatment has a promising effect against cotton wilt disease that could be combined with known plant protection strategies.

 The aim of this study is to investigate the antifungal activity of propolis against V. dahliae Kleb. under both in vitro and in vivo conditions. Firstly, the inhibitory effect of the propolis on mycelial growth in Potato Dextrose Agar PDA media containing its ethanol extract PE at various concentrations 0.003, 0.06, 0.125, 0.25, 0.5, and 1 ppmmL was investigated under in vitro conditions. Then to assess the effect of PE on non-defoliating PHCVd3 isolate and defoliating PHCVd47 isolate pathotypes of V. dahliae, the varieties Giza 45 resistant, Carmen tolerant, and Acala SJ2 susceptible treated with PEE 1 ppmmL were observed in the plant growth chamber up to the 4-6 leaf stage. The whole in vitro experiments were carried out with three replicates, and the studies in vivo experiment were with five replicates depending on a completely randomized parcels design. The most effective dose of PEE with1 ppmmL dose resulted in 75.2 suppression against the PHCVd3 isolate, while the effect of the same dose against the PHCVd47 isolate was 74.4. The lowest disease severity index DSI values against PHCVd3 and PHCVd47 isolates in cotton cultivars treated with PE were 1.34 and 1.64 in the Giza 45, respectively, and the highest DSI values were 3.80 and 3.90 in the Acala SJ2 cultivar in vivo experiment, respectively. The findings indicate that PE treatment has a promising effect against cotton wilt disease that could be combined with known plant protection strategies.

 The aim of this study is to investigate the antifungal activity of propolis against V. dahliae Kleb. under both in vitro and in vivo conditions. Firstly, the inhibitory effect of the propolis on mycelial growth in Potato Dextrose Agar PDA media containing its ethanol extract PE at various concentrations 0.003, 0.06, 0.125, 0.25, 0.5, and 1 ppmmL was investigated under in vitro conditions. Then to assess the effect of PE on non-defoliating PHCVd3 isolate and defoliating PHCVd47 isolate pathotypes of V. dahliae, the varieties Giza 45 resistant, Carmen tolerant, and Acala SJ2 susceptible treated with PEE 1 ppmmL were observed in the plant growth chamber up to the 4-6 leaf stage. The whole in vitro experiments were carried out with three replicates, and the studies in vivo experiment were with five replicates depending on a completely randomized parcels design. The most effective dose of PEE with1 ppmmL dose resulted in 75.2 suppression against the PHCVd3 isolate, while the effect of the same dose against the PHCVd47 isolate was 74.4. The lowest disease severity index DSI values against PHCVd3 and PHCVd47 isolates in cotton cultivars treated with PE were 1.34 and 1.64 in the Giza 45, respectively, and the highest DSI values were 3.80 and 3.90 in the Acala SJ2 cultivar in vivo experiment, respectively. The findings indicate that PE treatment has a promising effect against cotton wilt disease that could be combined with known plant protection strategies.

Makale detayları

The antifungal effect of propolis extract against cotton wild disease (Verticillium dahliae Kleb.)

Yazarlar:

Yeşim Kara | Oktay Erdoğan

Yayıncı:

İzzet KARA

Ana Başlıklar:

Structural Biology

Dergi:

International Journal of Secondary Metabolite Vol.10 Issue 2, pp.257-268

Belge Tipi:

Makale

Yayın Tarihi:

6/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001289448

pISSN:

2148-6905

Propolis

Verticillium dahliae

Antifungal activity

Alternative controlPropolis

Verticillium dahliae

Antifungal activity

Alternative control

Sayı

Makale detayları

Yazarlar: Yeşim Kara | Oktay Erdoğan

Yayıncı: İzzet KARA

Ana Başlıklar: Structural Biology

Dergi: International Journal of Secondary Metabolite Vol.10 Issue 2, pp.257-268

Belge Tipi: Makale

Yayın Tarihi: 6/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001289448

pISSN: 2148-6905

Sayı

Yazarlar:

Yeşim Kara | Oktay Erdoğan

Yayıncı:

İzzet KARA

Ana Başlıklar:

Structural Biology

Dergi:

International Journal of Secondary Metabolite Vol.10 Issue 2, pp.257-268

Belge Tipi:

Makale

Yayın Tarihi:

6/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001289448

pISSN:

2148-6905