Cardioprotective effect of vitamin D and melatonin on doxorubicin-induced cardiotoxicity in rat model: an electrocardiographic, scintigraphic and biochemical study

Serdar Savaş GÜL; Hatice AYGÜN

doi:https://doi.org/10.18621/eurj.410029

Makale detayları

Cardioprotective effect of vitamin D and melatonin on doxorubicin-induced cardiotoxicity in rat model: an electrocardiographic, scintigraphic and biochemical study

Yazarlar:

Serdar Savaş GÜL | Hatice AYGÜN

Yayıncı:

Prusa Medical Publishing

Ana Başlıklar:

Sağlık teşvikleri

Dergi:

The European Research Journal 2019 Vol.5 Issue 4, pp.649-657

Belge Tipi:

Makale

Yayın Tarihi:

7/2019

Belge Dili:

İngilizce

Document ID (Org No.):

2000662676

Sıklık:

İki Aylık

eISSN:

2149-3189

DOI:

https://doi.org/10.18621/eurj.410029

Özet:

 


Objectives: Doxorubicin
(DOX) is an antineoplastic drug that is widely used in
chemotherapy but its cardiotoxicity is the most important side effect that limits the clinical use of this drug. We
investigated DOX treatment and the effects of vitamin D and melatonin on heart
by electrocardiography, scintigraphic and biochemical methods. 


Methods: In
this study, forty-nine adult male Wistar albino
rats (220 ± 15 g) were randomly divided
into seven groups (n  =  7 each), namely control (CON, n = 7), doxorubicin (DOX,
n = 7), melatonin (MEL, n = 7), vitamin D (Vit D, n = 7), doxorubicin plus melatonin (DOX+MEL,
n = 7), doxorubicin
plus
vitamin D (DOX+Vit D, n = 7), and doxorubicin plus melatonin and vitamin D (DOX+MEL+Vit
D, n = 7) groups. Cardiotoxicity was induced by
intraperitoneal injection (i.p.) of DOX (18 mg/kg, i.p.) on the 15th,
16th and 17th days.
Rats receiving vitamin D and melatonin treatment in the DOX-induced
cardiotoxicity group received vitamin D (60,000 IU/kg, i.p.) were administered
in a single dose and melatonin (40 mg/kg/day, i.p.) for 17 days and were
injected with (18 mg/kg, i.p.) on doxorubicin 15th,
16th, and 17th days. On the 18th day
electrocardiography (ECG), 99mTechnetium pyrophosphate scintigraphy
and biochemical parameters were assessed. 


Results: DOX
caused changes in the ECG pattern, a significant decrease in heartbeat (p < 0.01), P wave (p < 0.001) and QRS complex durations
(p < 0.001), R wave amplitude (p < 0.001); elevation in ST-segment (p < 0.001) and decrease in QT
interval (p < 0,001),  and R-R interval durations (p < 0.001); increase in the serum levels of cardiac injury markers
(CK, BUN, cardiac troponin T), (p < 0.01), and increased 99mTechnetium pyrophosphate
uptake (p < 0.001) as
compared to the CON group. MEL,
Vit D and MEL+Vit D administration showed a same protective effect against
DOX-induced altered ECG pattern. Pre-treatment with MEL, Vit D and MEL+Vit D significantly
protected the heart from the toxic effect of DOX, by decreasing the levels of of cardiac injury markers (CK, BUN, cardiac troponin
T) (p < 0.001) and
decreased the elevated level of 99mTechnetium pyrophosphate
uptake (p < 0.001).









Conclusion: Vitamin D and melatonin treatment
prevented all the parameters of DOX-induced
cardiotoxicity in rats. 


.

Makale detayları

Cardioprotective effect of vitamin D and melatonin on doxorubicin-induced cardiotoxicity in rat model: an electrocardiographic, scintigraphic and biochemical study

Yazarlar: Serdar Savaş GÜL | Hatice AYGÜN

Yayıncı: Prusa Medical Publishing

Ana Başlıklar: Sağlık teşvikleri

Dergi: The European Research Journal 2019 Vol.5 Issue 4, pp.649-657

Belge Tipi: Makale

Yayın Tarihi: 7/2019

Belge Dili: İngilizce

Document ID (Org No.): 2000662676

Sıklık: İki Aylık

eISSN: 2149-3189

DOI: https://doi.org/10.18621/eurj.410029

Sayı

Yazarlar:

Serdar Savaş GÜL | Hatice AYGÜN

Yayıncı:

Prusa Medical Publishing

Ana Başlıklar:

Sağlık teşvikleri

Dergi:

The European Research Journal 2019 Vol.5 Issue 4, pp.649-657

Belge Tipi:

Makale

Yayın Tarihi:

7/2019

Belge Dili:

İngilizce

Document ID (Org No.):

2000662676

Sıklık:

İki Aylık

eISSN:

2149-3189

DOI:

https://doi.org/10.18621/eurj.410029