Investigation of the Effects of Cerium Nitrate and Platelet-Rich Plasma Treatments on Lung Tissue Following Burn Injury in Rats Using Histopathological and Immunohistochemical Methods

İsmail Bolat; Esra Manavoğlu Kirman; Kübra Asena Terim Kapakin

Makale detayları

Investigation of the Effects of Cerium Nitrate and Platelet-Rich Plasma Treatments on Lung Tissue Following Burn Injury in Rats Using Histopathological and Immunohistochemical Methods

Yazarlar:

İsmail Bolat | Esra Manavoğlu Kirman | Kübra Asena Terim Kapakin

Yayıncı:

Ataturk University

Ana Başlıklar:

Hayvan Bilimi (Diğer)

Dergi:

Laboratuvar Hayvanları Bilimi ve Uygulamaları Dergisi Vol.4 Issue 1, pp.26-32

Belge Tipi:

Makale

Yayın Tarihi:

3/2024

Belge Dili:

Türkçe

Document ID (Org No.):

2001314233

pISSN:

2791-8645

Anahtar kelimeler:

Özet:

 Burn injuries are among the most common physical traumas seen today. Although burns initially appear as damage to the skin, they can actually affect various tissues and organs due to the systemic inflammatory reactions they trigger. One of the primary organs that are rich in blood circulation is the lung tissue. Several natural compounds have been studied for burn wound treatment, among which cerium nitrate CN and Platelet-Rich Plasma PRP are known for their anti-inflammatory, antioxidant, and antiapoptotic properties. Despite their use in burn treatment, their specific effects on lung tissue following burns remain unclear. Therefore, this study aimed to investigate the protective effects of CN and PRP against damage in lung tissue following thermal burns on the skin. In this study, thermal burn injuries were induced on the skin of rats. In the CN group, rats were exposed to a 0.04 M molar cerium nitrate bath solution for 30 minutes after the burn. In the PRP group, 0.1 ml of PRP was subcutaneously injected into the wound areas of the rats. On the 21st day of the study, rats were sacrificed under general anesthesia, and lung tissues were collected. Histopathological analysis of lung tissues after burns revealed bleeding and mononuclear cell infiltrations in the interalveolar regions, leading to thickening. Mononuclear cell infiltrations were also observed around the bronchioles. Immunohistochemical examinations showed severe expressions of Tnf-α and 8-OHdG in lung tissues after burns. However, in the groups treated with CN and PRP, the histopathological and immunohistochemical findings approached normal values after burn injury. In conclusion, CN and PRP demonstrated protective effects against damage in lung tissue following burn injury.

 Burn injuries are among the most common physical traumas seen today. Although burns initially appear as damage to the skin, they can actually affect various tissues and organs due to the systemic inflammatory reactions they trigger. One of the primary organs that are rich in blood circulation is the lung tissue. Several natural compounds have been studied for burn wound treatment, among which cerium nitrate CN and Platelet-Rich Plasma PRP are known for their anti-inflammatory, antioxidant, and antiapoptotic properties. Despite their use in burn treatment, their specific effects on lung tissue following burns remain unclear. Therefore, this study aimed to investigate the protective effects of CN and PRP against damage in lung tissue following thermal burns on the skin. In this study, thermal burn injuries were induced on the skin of rats. In the CN group, rats were exposed to a 0.04 M molar cerium nitrate bath solution for 30 minutes after the burn. In the PRP group, 0.1 ml of PRP was subcutaneously injected into the wound areas of the rats. On the 21st day of the study, rats were sacrificed under general anesthesia, and lung tissues were collected. Histopathological analysis of lung tissues after burns revealed bleeding and mononuclear cell infiltrations in the interalveolar regions, leading to thickening. Mononuclear cell infiltrations were also observed around the bronchioles. Immunohistochemical examinations showed severe expressions of Tnf-α and 8-OHdG in lung tissues after burns. However, in the groups treated with CN and PRP, the histopathological and immunohistochemical findings approached normal values after burn injury. In conclusion, CN and PRP demonstrated protective effects against damage in lung tissue following burn injury.

 Burn injuries are among the most common physical traumas seen today. Although burns initially appear as damage to the skin, they can actually affect various tissues and organs due to the systemic inflammatory reactions they trigger. One of the primary organs that are rich in blood circulation is the lung tissue. Several natural compounds have been studied for burn wound treatment, among which cerium nitrate CN and Platelet-Rich Plasma PRP are known for their anti-inflammatory, antioxidant, and antiapoptotic properties. Despite their use in burn treatment, their specific effects on lung tissue following burns remain unclear. Therefore, this study aimed to investigate the protective effects of CN and PRP against damage in lung tissue following thermal burns on the skin. In this study, thermal burn injuries were induced on the skin of rats. In the CN group, rats were exposed to a 0.04 M molar cerium nitrate bath solution for 30 minutes after the burn. In the PRP group, 0.1 ml of PRP was subcutaneously injected into the wound areas of the rats. On the 21st day of the study, rats were sacrificed under general anesthesia, and lung tissues were collected. Histopathological analysis of lung tissues after burns revealed bleeding and mononuclear cell infiltrations in the interalveolar regions, leading to thickening. Mononuclear cell infiltrations were also observed around the bronchioles. Immunohistochemical examinations showed severe expressions of Tnf-α and 8-OHdG in lung tissues after burns. However, in the groups treated with CN and PRP, the histopathological and immunohistochemical findings approached normal values after burn injury. In conclusion, CN and PRP demonstrated protective effects against damage in lung tissue following burn injury.

 Yanık, günümüzde en yaygın görülen fiziksel travmaların başında gelmektedir. Yanık ilk bakışta deride oluşan bir hasar olarak görülse de aslında vücudun genelinden oluşturmuş olduğu sistemik yangısal reaksiyonlar sonucunda birçok doku ve organı etkileyebilmektedir. Bunların başında ise kan dolaşımı yönünden zengin olan akciğer dokusu yer almaktadır. Yanık yarası tedavisinde birçok doğal bileşen çalışmalarda kullanılmıştır. Antiinflamatuvar, antioksidan ve antiapoptotik etkinlikleri bilinen Seryum Nitrat SN ve Plateletten Zengin Plazma PZP bu doğal maddeler arasında yer almaktadır. Bu doğal maddeler her ne kadar yanık tedavisinde kullanılsa da yanık sonrası akciğer dokularında nasıl bir etki ettiği net bir şekilde bilinmemektedir. Bu sebeple, deride oluşturulan termal yanık sonrasında akciğer dokusunda meydana gelen hasara karşı SN ve PZP nin koruyucu etkinlikleri bu çalışmada araştırıldı. Ratlarda deride çalışmanın ilk gününde termal yanık hasarı oluşturuldu. SN grubunda yanık sonrası 0.04 molar Seryum Nitrat banyo solüsyonunda ratlar 30 dakika boyunca bekletildi. PZP grubunda ise ratların yara bölgelerine 0.1 mllik PZP deri altı enjekte edildi. Çalışmanın 21. gününün sonunda ratlar genel anestezi altında sakrifiye edildi ve akciğer dokuları alındı. Akciğer dokularında yanık sonrasında histopatolojik olarak; interalveoler bölgelerde kanama ile mononükleer hücre infiltrasyonları ve buna bağlı olarak kalınlaşma gözlendi. Bronşiollerin etrafında mononükleer hücre infiltrasyonları görüldü. İmmünohistokimyasal incelemelerde; yanık sonrası akciğer dokularında şiddetli Tnf-α ve 8-OHdG ekspresyonları gözlendi. SN ve PZP uygulanan gruplarda ise yanık sonrası oluşan histopatolojik ve immünohistokimyasal bulguların normal değerlere yaklaştığı tespit edildi. Sonuç olarak; yanık sonrası akciğer dokusunda oluşan hasarlara karşı SN ve PZP nin koruyucu etkinliği olduğu ortaya konuldu.

Makale detayları

Investigation of the Effects of Cerium Nitrate and Platelet-Rich Plasma Treatments on Lung Tissue Following Burn Injury in Rats Using Histopathological and Immunohistochemical Methods

Yazarlar:

İsmail Bolat | Esra Manavoğlu Kirman | Kübra Asena Terim Kapakin

Yayıncı:

Ataturk University

Ana Başlıklar:

Hayvan Bilimi (Diğer)

Dergi:

Laboratuvar Hayvanları Bilimi ve Uygulamaları Dergisi Vol.4 Issue 1, pp.26-32

Belge Tipi:

Makale

Yayın Tarihi:

3/2024

Belge Dili:

Türkçe

Document ID (Org No.):

2001314233

pISSN:

2791-8645

Burn

cerium nitrate

inflammation

lung

platelet-rich plasma.Akciğer

inflamasyon

plateletten zengin plazma

seryum nitrat

yanık.

Sayı

Makale detayları

Yazarlar: İsmail Bolat | Esra Manavoğlu Kirman | Kübra Asena Terim Kapakin

Yayıncı: Ataturk University

Ana Başlıklar: Hayvan Bilimi (Diğer)

Dergi: Laboratuvar Hayvanları Bilimi ve Uygulamaları Dergisi Vol.4 Issue 1, pp.26-32

Belge Tipi: Makale

Yayın Tarihi: 3/2024

Belge Dili: Türkçe

Document ID (Org No.): 2001314233

pISSN: 2791-8645

Sayı

Yazarlar:

İsmail Bolat | Esra Manavoğlu Kirman | Kübra Asena Terim Kapakin

Yayıncı:

Ataturk University

Ana Başlıklar:

Hayvan Bilimi (Diğer)

Dergi:

Laboratuvar Hayvanları Bilimi ve Uygulamaları Dergisi Vol.4 Issue 1, pp.26-32

Belge Tipi:

Makale

Yayın Tarihi:

3/2024

Belge Dili:

Türkçe

Document ID (Org No.):

2001314233

pISSN:

2791-8645