Effect of Astragalus and Astragalus Polysaccharide on Antimicrobial Peptides PR-39 and Protegrin-1 Gene Expression in Pigs

Youjin TU; Chunlan XU; Yizhen WANG; Guojia YAO; Tizhong SHAN

Makale detayları

Effect of Astragalus and Astragalus Polysaccharide on Antimicrobial Peptides PR-39 and Protegrin-1 Gene Expression in Pigs

Yazarlar:

Youjin TU | Chunlan XU | Yizhen WANG | Guojia YAO | Tizhong SHAN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2006 Vol.30 Issue 3, pp.325-330

Belge Tipi:

Makale

Yayın Tarihi:

2/2006

Belge Dili:

Türkçe

Document ID (Org No.):

2000768440

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

Anahtar kelimeler:

Özet:

 

This experiment was conducted to study the effects of micron Astragalus, 80 mesh Astragalus and Astragalus polysaccharide (APS) on antimicrobial peptides PR-39 and protegrin-1 gene expression in pigs using semi-quantitative RT-PCR analysis. One hundred twenty 60-day-old Duroc x Landrace x Yorkshire piglets (21.88 ± 1.26 kg of average initial body weight) were randomly allotted to 4 treatments. Each treatment had 3 replicates (i.e. pens) with 10 pigs per pen. The basal diets were supplemented with 0, 5 g kg-1 APS, 5 g kg-1 80 mesh Astragalus, and 5 g kg-1 micron Astragalus, respectively, and the feeding experiment lasted 30 days. After the feeding experiment, 12 pigs with 3 animals in each treatment were chosen to determine the effect of Astragalus or APS on PR-39 and protegrin-1 mRNA expression of pigs. The results showed that supplementation with 5 g kg-1 micron Astragalus significantly (P < 0.05) enhanced antimicrobial peptides PR-39 and protegrin-1 gene expression. Pigs fed the diet containing 5 g kg-1 80 mesh Astragalus also had higher PR-39 and protegrin-1 gene expression level than did the control (P < 0.05). However, micron Astragalus was more effective than 80 mesh Astragalus in enhancing PR-39 and protegrin-1 gene expression. Supplementation with 5 g kg-1 APS reduced PR-39 and protegrin-1 gene expression (P < 0.05).

 

This experiment was conducted to study the effects of micron Astragalus, 80 mesh Astragalus and Astragalus polysaccharide (APS) on antimicrobial peptides PR-39 and protegrin-1 gene expression in pigs using semi-quantitative RT-PCR analysis. One hundred twenty 60-day-old Duroc x Landrace x Yorkshire piglets (21.88 ± 1.26 kg of average initial body weight) were randomly allotted to 4 treatments. Each treatment had 3 replicates (i.e. pens) with 10 pigs per pen. The basal diets were supplemented with 0, 5 g kg-1 APS, 5 g kg-1 80 mesh Astragalus, and 5 g kg-1 micron Astragalus, respectively, and the feeding experiment lasted 30 days. After the feeding experiment, 12 pigs with 3 animals in each treatment were chosen to determine the effect of Astragalus or APS on PR-39 and protegrin-1 mRNA expression of pigs. The results showed that supplementation with 5 g kg-1 micron Astragalus significantly (P < 0.05) enhanced antimicrobial peptides PR-39 and protegrin-1 gene expression. Pigs fed the diet containing 5 g kg-1 80 mesh Astragalus also had higher PR-39 and protegrin-1 gene expression level than did the control (P < 0.05). However, micron Astragalus was more effective than 80 mesh Astragalus in enhancing PR-39 and protegrin-1 gene expression. Supplementation with 5 g kg-1 APS reduced PR-39 and protegrin-1 gene expression (P < 0.05).

Makale detayları

Effect of Astragalus and Astragalus Polysaccharide on Antimicrobial Peptides PR-39 and Protegrin-1 Gene Expression in Pigs

Yazarlar: Youjin TU | Chunlan XU | Yizhen WANG | Guojia YAO | Tizhong SHAN

Yayıncı: TUBITAK

Dergi: Turkish Journal of Veterinary and Animal Sciences 2006 Vol.30 Issue 3, pp.325-330

Belge Tipi: Makale

Yayın Tarihi: 2/2006

Belge Dili: Türkçe

Document ID (Org No.): 2000768440

Sıklık: İki Aylık

eISSN: 1303-6181

pISSN: 1300-0128

Astragalus

PR-39

protegrin-1

antimicrobial peptide

pigs

Sayı

Yazarlar:

Youjin TU | Chunlan XU | Yizhen WANG | Guojia YAO | Tizhong SHAN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2006 Vol.30 Issue 3, pp.325-330

Belge Tipi:

Makale

Yayın Tarihi:

2/2006

Belge Dili:

Türkçe

Document ID (Org No.):

2000768440

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128