Effects of experimental diabetes on testis proliferations and apoptosis in rats

Cevat AKTAS; Mehmet KANTER; Mustafa ERBOĞA; Hüseyin TİMURKAN

Makale detayları

Effects of experimental diabetes on testis proliferations and apoptosis in rats

Yazarlar:

Cevat AKTAS | Mehmet KANTER | Mustafa ERBOĞA | Hüseyin TİMURKAN

Yayıncı:

Ondokuz Mayıs University

Dergi:

Journal of Experimental and Clinical Medicine 2011 Vol.28 Issue 3, pp.94-98

Belge Tipi:

Makale

Yayın Tarihi:

2/2012

Belge Dili:

İngilizce

Document ID (Org No.):

2000509651

Sıklık:

Üç Aylık

eISSN:

1309-5129

pISSN:

1309-4483

Özet:

 

ABSTRACT
This work aimed to investigate the role of diabetes on cell proliferation, and apoptosis in testis. The rats were randomly allotted into one of two experimental groups: control and diabetic group; each group contain 10 animals. Diabetes was induced by a single intra¬peritoneal injection of STZ (50 mg/kg). Testicular damage was examined by using he¬matoxylin and eosin, immunohistochemical staining of proliferating cell nuclear antigen (PCNA), and apoptosis was determined by terminal-deoxynucleotidyl-transferase medi¬ated dUTP nick end labeling (TUNEL). Potential disorders associated with seminiferous tubular sperm formation were evaluated using the Johnsen score. The mean seminiferous tubule diameter (MSTD) and mean testicular biopsy score (MTBS) values were signifi¬cantly decreased in diabetic group was compared to the control group. Our data indicate a significant reduction in the expression of PCNA and an enhancement in the activity of TUNEL in testis tissues of the diabetic group. The effects of diabetes on spermatogenesis can be clearly detected as a testicular cell death and decrease in MTBS, MSTD, and PCNA expression. 
J. Exp. Clin. Med., 2011; 28:94-98.

Makale detayları

Effects of experimental diabetes on testis proliferations and apoptosis in rats

Yazarlar: Cevat AKTAS | Mehmet KANTER | Mustafa ERBOĞA | Hüseyin TİMURKAN

Yayıncı: Ondokuz Mayıs University

Dergi: Journal of Experimental and Clinical Medicine 2011 Vol.28 Issue 3, pp.94-98

Belge Tipi: Makale

Yayın Tarihi: 2/2012

Belge Dili: İngilizce

Document ID (Org No.): 2000509651

Sıklık: Üç Aylık

eISSN: 1309-5129

pISSN: 1309-4483

Sayı

Yazarlar:

Cevat AKTAS | Mehmet KANTER | Mustafa ERBOĞA | Hüseyin TİMURKAN

Yayıncı:

Ondokuz Mayıs University

Dergi:

Journal of Experimental and Clinical Medicine 2011 Vol.28 Issue 3, pp.94-98

Belge Tipi:

Makale

Yayın Tarihi:

2/2012

Belge Dili:

İngilizce

Document ID (Org No.):

2000509651

Sıklık:

Üç Aylık

eISSN:

1309-5129

pISSN:

1309-4483