Biological and Immunological Properties of Mesenchymal Stromal Cells Derived From Bone Marrow in Childhood Acute Myeloid Leukemia

İlkay Pişkin; Tuba Özdemir Sancı; Yasin Köksal; Hüsniye Neşe Yaralı; Fatma Karaca Kara; Bahattin Tunç; Meltem Özgüner

Makale detayları

Biological and Immunological Properties of Mesenchymal Stromal Cells Derived From Bone Marrow in Childhood Acute Myeloid Leukemia

Yazarlar:

İlkay Pişkin | Tuba Özdemir Sancı | Yasin Köksal | Hüsniye Neşe Yaralı | Fatma Karaca Kara | Bahattin Tunç | Meltem Özgüner

Yayıncı:

Istanbul University

Ana Başlıklar:

Clinical Sciences (Other)

Dergi:

Experimed Vol.15 Issue 1, pp.66-76

Belge Tipi:

Makale

Yayın Tarihi:

4/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001348954

pISSN:

2667-5846

Anahtar kelimeler:

Özet:

 Objective: Pediatric acute myeloid leukemia AML is a common form of pediatric leukemia and is characterized by the accumulation of abnormal white blood cells, called blasts, in the bone marrow BM. The aim of this study was to understand the BM microenvironment by studying the biological and immunological properties of BM-derived mesenchymal stromal cells MSCs and mononuclear cells MNCs to elucidate the potential role of phytohaemag glutinin in cell viability. Materials and Methods: BM and peripheral blood samples were obtained from seven pediatric AML patients and seven donors. BM-MSCs and MNCs were isolated and characterized. Population doubling PD values, adipogenic and osteogenic differentiation capacity, cell viability, phytohemagglutinin PHA assay, and flow cytometry were performed. Results: Mononucleated cells from peripheral blood of AML patients and donors and T-cell activation markers CD3CD69, CD4C25, CD3HLA-DR were measured by flow cytometry 22.184; p0.823. BM-MSCs were co-cultured with MNCs, and PD values for AML patients were similar to those of donors z1.074; p0.394. It was statistically significant when healthy MNC and healthy MNC PHA groups were compared p0.015. When healthy MNC PHA and healthy MSCAML MNC PHA groups were compared, it was found to be statistically significant p0.014. Conclusion: This interaction is also not unidirectional. This interaction serves as a marker for understanding the immunological effects of AML.

 Objective: Pediatric acute myeloid leukemia AML is a common form of pediatric leukemia and is characterized by the accumulation of abnormal white blood cells, called blasts, in the bone marrow BM. The aim of this study was to understand the BM microenvironment by studying the biological and immunological properties of BM-derived mesenchymal stromal cells MSCs and mononuclear cells MNCs to elucidate the potential role of phytohaemag glutinin in cell viability. Materials and Methods: BM and peripheral blood samples were obtained from seven pediatric AML patients and seven donors. BM-MSCs and MNCs were isolated and characterized. Population doubling PD values, adipogenic and osteogenic differentiation capacity, cell viability, phytohemagglutinin PHA assay, and flow cytometry were performed. Results: Mononucleated cells from peripheral blood of AML patients and donors and T-cell activation markers CD3CD69, CD4C25, CD3HLA-DR were measured by flow cytometry 22.184; p0.823. BM-MSCs were co-cultured with MNCs, and PD values for AML patients were similar to those of donors z1.074; p0.394. It was statistically significant when healthy MNC and healthy MNC PHA groups were compared p0.015. When healthy MNC PHA and healthy MSCAML MNC PHA groups were compared, it was found to be statistically significant p0.014. Conclusion: This interaction is also not unidirectional. This interaction serves as a marker for understanding the immunological effects of AML.

 Objective: Pediatric acute myeloid leukemia AML is a common form of pediatric leukemia and is characterized by the accumulation of abnormal white blood cells, called blasts, in the bone marrow BM. The aim of this study was to understand the BM microenvironment by studying the biological and immunological properties of BM-derived mesenchymal stromal cells MSCs and mononuclear cells MNCs to elucidate the potential role of phytohaemag glutinin in cell viability. Materials and Methods: BM and peripheral blood samples were obtained from seven pediatric AML patients and seven donors. BM-MSCs and MNCs were isolated and characterized. Population doubling PD values, adipogenic and osteogenic differentiation capacity, cell viability, phytohemagglutinin PHA assay, and flow cytometry were performed. Results: Mononucleated cells from peripheral blood of AML patients and donors and T-cell activation markers CD3CD69, CD4C25, CD3HLA-DR were measured by flow cytometry 22.184; p0.823. BM-MSCs were co-cultured with MNCs, and PD values for AML patients were similar to those of donors z1.074; p0.394. It was statistically significant when healthy MNC and healthy MNC PHA groups were compared p0.015. When healthy MNC PHA and healthy MSCAML MNC PHA groups were compared, it was found to be statistically significant p0.014. Conclusion: This interaction is also not unidirectional. This interaction serves as a marker for understanding the immunological effects of AML.

 Objective: Pediatric acute myeloid leukemia AML is a common form of pediatric leukemia and is characterized by the accumulation of abnormal white blood cells, called blasts, in the bone marrow BM. The aim of this study was to understand the BM microenvironment by studying the biological and immunological properties of BM-derived mesenchymal stromal cells MSCs and mononuclear cells MNCs to elucidate the potential role of phytohaemag glutinin in cell viability. Materials and Methods: BM and peripheral blood samples were obtained from seven pediatric AML patients and seven donors. BM-MSCs and MNCs were isolated and characterized. Population doubling PD values, adipogenic and osteogenic differentiation capacity, cell viability, phytohemagglutinin PHA assay, and flow cytometry were performed. Results: Mononucleated cells from peripheral blood of AML patients and donors and T-cell activation markers CD3CD69, CD4C25, CD3HLA-DR were measured by flow cytometry 22.184; p0.823. BM-MSCs were co-cultured with MNCs, and PD values for AML patients were similar to those of donors z1.074; p0.394. It was statistically significant when healthy MNC and healthy MNC PHA groups were compared p0.015. When healthy MNC PHA and healthy MSCAML MNC PHA groups were compared, it was found to be statistically significant p0.014. Conclusion: This interaction is also not unidirectional. This interaction serves as a marker for understanding the immunological effects of AML.

Makale detayları

Biological and Immunological Properties of Mesenchymal Stromal Cells Derived From Bone Marrow in Childhood Acute Myeloid Leukemia

Yazarlar:

İlkay Pişkin | Tuba Özdemir Sancı | Yasin Köksal | Hüsniye Neşe Yaralı | Fatma Karaca Kara | Bahattin Tunç | Meltem Özgüner

Yayıncı:

Istanbul University

Ana Başlıklar:

Clinical Sciences (Other)

Dergi:

Experimed Vol.15 Issue 1, pp.66-76

Belge Tipi:

Makale

Yayın Tarihi:

4/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001348954

pISSN:

2667-5846

Pediatric AML

BM-MSCs

PHA

Cell viability

MNCs

Sayı

Makale detayları

Biological and Immunological Properties of Mesenchymal Stromal Cells Derived From Bone Marrow in Childhood Acute Myeloid Leukemia

Yazarlar: İlkay Pişkin | Tuba Özdemir Sancı | Yasin Köksal | Hüsniye Neşe Yaralı | Fatma Karaca Kara | Bahattin Tunç | Meltem Özgüner

Yayıncı: Istanbul University

Ana Başlıklar: Clinical Sciences (Other)

Dergi: Experimed Vol.15 Issue 1, pp.66-76

Belge Tipi: Makale

Yayın Tarihi: 4/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001348954

pISSN: 2667-5846

Pediatric AML

BM-MSCs

PHA

Cell viability

MNCs

Sayı

Yazarlar:

İlkay Pişkin | Tuba Özdemir Sancı | Yasin Köksal | Hüsniye Neşe Yaralı | Fatma Karaca Kara | Bahattin Tunç | Meltem Özgüner

Yayıncı:

Istanbul University

Ana Başlıklar:

Clinical Sciences (Other)

Dergi:

Experimed Vol.15 Issue 1, pp.66-76

Belge Tipi:

Makale

Yayın Tarihi:

4/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001348954

pISSN:

2667-5846