Revised classification design of the Anatolian species of Nannospalax (Rodentia: Spalacidae) using RFLP analysis

Teoman KANKILIÇ; Canan GÜRPINAR

Makale detayları

Revised classification design of the Anatolian species of Nannospalax (Rodentia: Spalacidae) using RFLP analysis

Yazarlar:

Teoman KANKILIÇ | Canan GÜRPINAR

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Zoology 2014 Vol.38 Issue 1, pp.67-78

Belge Tipi:

Makale

Yayın Tarihi:

5/2014

Belge Dili:

Türkçe

Document ID (Org No.):

2000775763

Sıklık:

İki Aylık

eISSN:

1303-6114

pISSN:

1300-0179

Anahtar kelimeler:

Özet:

 

Genetic differences in 2 regions (D-loop and cytochrome b) of mitochondrial DNA in Nannospalax xanthodon and Nannospalax ehrenbergi were determined using restriction fragment length polymorphism (RFLP) analysis. For 2 species distributed in Anatolia, 94 specimens from 62 populations of Nannospalax xanthodon and 2 populations of Nannospalax ehrenbergi were studied. To reveal genetic differences, the D-loop (626 bp) and cytochrome b (500 bp) regions of mitochondrial DNA were amplified and then cut by 4 different restriction endonuclease enzymes (BamH-I, Taq-I, Alu-I, and Msp-I). RFLP analysis revealed 4 haplotypes for the D-loop region and 8 haplotypes for the cytochrome b region in 94 specimens. According to the results of this study, N. nehringi, which is distributed in eastern Anatolia, is a valid species and not a synonym of N. xanthodon. A total of 2 additional species (N. nehringi and N. labaumei) occur with N. xanthodon and N. ehrenbergi; thus, Anatolian blind mole rats cannot be represented by only 2 species.

 

Genetic differences in 2 regions (D-loop and cytochrome b) of mitochondrial DNA in Nannospalax xanthodon and Nannospalax ehrenbergi were determined using restriction fragment length polymorphism (RFLP) analysis. For 2 species distributed in Anatolia, 94 specimens from 62 populations of Nannospalax xanthodon and 2 populations of Nannospalax ehrenbergi were studied. To reveal genetic differences, the D-loop (626 bp) and cytochrome b (500 bp) regions of mitochondrial DNA were amplified and then cut by 4 different restriction endonuclease enzymes (BamH-I, Taq-I, Alu-I, and Msp-I). RFLP analysis revealed 4 haplotypes for the D-loop region and 8 haplotypes for the cytochrome b region in 94 specimens. According to the results of this study, N. nehringi, which is distributed in eastern Anatolia, is a valid species and not a synonym of N. xanthodon. A total of 2 additional species (N. nehringi and N. labaumei) occur with N. xanthodon and N. ehrenbergi; thus, Anatolian blind mole rats cannot be represented by only 2 species.

Makale detayları

Revised classification design of the Anatolian species of Nannospalax (Rodentia: Spalacidae) using RFLP analysis

Yazarlar:

Teoman KANKILIÇ | Canan GÜRPINAR

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Zoology 2014 Vol.38 Issue 1, pp.67-78

Belge Tipi:

Makale

Yayın Tarihi:

5/2014

Belge Dili:

Türkçe

Document ID (Org No.):

2000775763

Sıklık:

İki Aylık

eISSN:

1303-6114

pISSN:

1300-0179

Key words: Nannospalax

mtDNA

Turkey

Sayı

Makale detayları

Revised classification design of the Anatolian species of Nannospalax (Rodentia: Spalacidae) using RFLP analysis

Yazarlar: Teoman KANKILIÇ | Canan GÜRPINAR

Yayıncı: TUBITAK

Dergi: Turkish Journal of Zoology 2014 Vol.38 Issue 1, pp.67-78

Belge Tipi: Makale

Yayın Tarihi: 5/2014

Belge Dili: Türkçe

Document ID (Org No.): 2000775763

Sıklık: İki Aylık

eISSN: 1303-6114

pISSN: 1300-0179

Key words: Nannospalax

mtDNA

Turkey

Sayı

Yazarlar:

Teoman KANKILIÇ | Canan GÜRPINAR

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Zoology 2014 Vol.38 Issue 1, pp.67-78

Belge Tipi:

Makale

Yayın Tarihi:

5/2014

Belge Dili:

Türkçe

Document ID (Org No.):

2000775763

Sıklık:

İki Aylık

eISSN:

1303-6114

pISSN:

1300-0179