Screening of Antibiotics Biodegradability from Wastewater

Souhila Saım

Makale detayları

Screening of Antibiotics Biodegradability from Wastewater

Yazarlar:

Souhila Saım

Yayıncı:

Istanbul University

Ana Başlıklar:

Structural Biology

Dergi:

European Journal of Biology Vol.82 Issue 1, pp.70-85

Belge Tipi:

Makale

Yayın Tarihi:

6/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001293320

pISSN:

2602-2575

Anahtar kelimeler:

Özet:

 Objective: One of the sources of environment antibiotics contamination is wastewater treatment plants WWTPs, there by constituting a global public health risk. This present study aimed to investigate the biodegradability of antibiotics and antiseptics and highlights the biodegradation of Ciprofloxacin as a sole carbon source by a bacterium isolated from the sludge El Kouwaer, WWTP located in Mascara. Materials and Methods: In the present study, biodegradability of some antibiotics and antiseptic were tested at 50 mgl concentration through active sludge microorganisms by Manometric Respirometry Method OECD 301F. Further analysis of 16S rRNA gene sequencing used to identify MK4 strain isolated from the sludge. Furthermore, ATR-FTIR spectroscopy was conducted in order to identify its biodegradation in the presence of different carbon sources and LCMSMS spectrometry were used to identify the metabolite degradation. Results: Our Results revealed that four antibiotics tested were readily biodegradable 60 as Ciprofloxacin, Doxycycline, Amoxicillin, Ampicillin, and Penicillin. Conversely, other was not readily biodegradable, such as Azithromycin 36.11, Cephalexin 36.20, and Metronidazole 33.33. Meanwhile, the remaining antibiotics under examination were degraded, with Sulfamethoxazole 25.75, Clarithromycin 25.36, and Nifuroxazide 16.33 recording degradation. Ciprofloxacin was chosen to represent the most biodegraded antibiotic. Based on 16S rRNA gene, MK4 strain was related to Klebsiella oxytoca 99.99. ATR-FTIR revealed that the strain K. oxytoca MK4 caused changes in the structure of the Ciprofloxacin, in the presence of various sources of carbon, with varying effects on bacterial growth and biodegradation. Conclusion: In this study, the identified strain K. oxytoca MK4 facilitated the degradation of Ciprofloxacin.

 Objective: One of the sources of environment antibiotics contamination is wastewater treatment plants WWTPs, there by constituting a global public health risk. This present study aimed to investigate the biodegradability of antibiotics and antiseptics and highlights the biodegradation of Ciprofloxacin as a sole carbon source by a bacterium isolated from the sludge El Kouwaer, WWTP located in Mascara. Materials and Methods: In the present study, biodegradability of some antibiotics and antiseptic were tested at 50 mgl concentration through active sludge microorganisms by Manometric Respirometry Method OECD 301F. Further analysis of 16S rRNA gene sequencing used to identify MK4 strain isolated from the sludge. Furthermore, ATR-FTIR spectroscopy was conducted in order to identify its biodegradation in the presence of different carbon sources and LCMSMS spectrometry were used to identify the metabolite degradation. Results: Our Results revealed that four antibiotics tested were readily biodegradable 60 as Ciprofloxacin, Doxycycline, Amoxicillin, Ampicillin, and Penicillin. Conversely, other was not readily biodegradable, such as Azithromycin 36.11, Cephalexin 36.20, and Metronidazole 33.33. Meanwhile, the remaining antibiotics under examination were degraded, with Sulfamethoxazole 25.75, Clarithromycin 25.36, and Nifuroxazide 16.33 recording degradation. Ciprofloxacin was chosen to represent the most biodegraded antibiotic. Based on 16S rRNA gene, MK4 strain was related to Klebsiella oxytoca 99.99. ATR-FTIR revealed that the strain K. oxytoca MK4 caused changes in the structure of the Ciprofloxacin, in the presence of various sources of carbon, with varying effects on bacterial growth and biodegradation. Conclusion: In this study, the identified strain K. oxytoca MK4 facilitated the degradation of Ciprofloxacin.

 Objective: One of the sources of environment antibiotics contamination is wastewater treatment plants WWTPs, there by constituting a global public health risk. This present study aimed to investigate the biodegradability of antibiotics and antiseptics and highlights the biodegradation of Ciprofloxacin as a sole carbon source by a bacterium isolated from the sludge El Kouwaer, WWTP located in Mascara. Materials and Methods: In the present study, biodegradability of some antibiotics and antiseptic were tested at 50 mgl concentration through active sludge microorganisms by Manometric Respirometry Method OECD 301F. Further analysis of 16S rRNA gene sequencing used to identify MK4 strain isolated from the sludge. Furthermore, ATR-FTIR spectroscopy was conducted in order to identify its biodegradation in the presence of different carbon sources and LCMSMS spectrometry were used to identify the metabolite degradation. Results: Our Results revealed that four antibiotics tested were readily biodegradable 60 as Ciprofloxacin, Doxycycline, Amoxicillin, Ampicillin, and Penicillin. Conversely, other was not readily biodegradable, such as Azithromycin 36.11, Cephalexin 36.20, and Metronidazole 33.33. Meanwhile, the remaining antibiotics under examination were degraded, with Sulfamethoxazole 25.75, Clarithromycin 25.36, and Nifuroxazide 16.33 recording degradation. Ciprofloxacin was chosen to represent the most biodegraded antibiotic. Based on 16S rRNA gene, MK4 strain was related to Klebsiella oxytoca 99.99. ATR-FTIR revealed that the strain K. oxytoca MK4 caused changes in the structure of the Ciprofloxacin, in the presence of various sources of carbon, with varying effects on bacterial growth and biodegradation. Conclusion: In this study, the identified strain K. oxytoca MK4 facilitated the degradation of Ciprofloxacin.

 Objective: One of the sources of environment antibiotics contamination is wastewater treatment plants WWTPs, there by constituting a global public health risk. This present study aimed to investigate the biodegradability of antibiotics and antiseptics and highlights the biodegradation of Ciprofloxacin as a sole carbon source by a bacterium isolated from the sludge El Kouwaer, WWTP located in Mascara. Materials and Methods: In the present study, biodegradability of some antibiotics and antiseptic were tested at 50 mgl concentration through active sludge microorganisms by Manometric Respirometry Method OECD 301F. Further analysis of 16S rRNA gene sequencing used to identify MK4 strain isolated from the sludge. Furthermore, ATR-FTIR spectroscopy was conducted in order to identify its biodegradation in the presence of different carbon sources and LCMSMS spectrometry were used to identify the metabolite degradation. Results: Our Results revealed that four antibiotics tested were readily biodegradable 60 as Ciprofloxacin, Doxycycline, Amoxicillin, Ampicillin, and Penicillin. Conversely, other was not readily biodegradable, such as Azithromycin 36.11, Cephalexin 36.20, and Metronidazole 33.33. Meanwhile, the remaining antibiotics under examination were degraded, with Sulfamethoxazole 25.75, Clarithromycin 25.36, and Nifuroxazide 16.33 recording degradation. Ciprofloxacin was chosen to represent the most biodegraded antibiotic. Based on 16S rRNA gene, MK4 strain was related to Klebsiella oxytoca 99.99. ATR-FTIR revealed that the strain K. oxytoca MK4 caused changes in the structure of the Ciprofloxacin, in the presence of various sources of carbon, with varying effects on bacterial growth and biodegradation. Conclusion: In this study, the identified strain K. oxytoca MK4 facilitated the degradation of Ciprofloxacin.

Makale detayları

Screening of Antibiotics Biodegradability from Wastewater

Yazarlar:

Souhila Saım

Yayıncı:

Istanbul University

Ana Başlıklar:

Structural Biology

Dergi:

European Journal of Biology Vol.82 Issue 1, pp.70-85

Belge Tipi:

Makale

Yayın Tarihi:

6/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001293320

pISSN:

2602-2575

Wastewater

antibiotic

antiseptics

biodegradability

ATR-FTIR spectroscopy

LCMS/MS metabolites.

Sayı

Makale detayları

Screening of Antibiotics Biodegradability from Wastewater

Yazarlar: Souhila Saım

Yayıncı: Istanbul University

Ana Başlıklar: Structural Biology

Dergi: European Journal of Biology Vol.82 Issue 1, pp.70-85

Belge Tipi: Makale

Yayın Tarihi: 6/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001293320

pISSN: 2602-2575

Wastewater

antibiotic

antiseptics

biodegradability

ATR-FTIR spectroscopy

LCMS/MS metabolites.

Sayı

Yazarlar:

Souhila Saım

Yayıncı:

Istanbul University

Ana Başlıklar:

Structural Biology

Dergi:

European Journal of Biology Vol.82 Issue 1, pp.70-85

Belge Tipi:

Makale

Yayın Tarihi:

6/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001293320

pISSN:

2602-2575