Conditioned media of tonsil derived mesenchymal stem cells shows different rates of cytotoxicity on solid cancer cells

Melek Yüce; Esra Albayrak; Çağrı Gümüşkaptan

Makale detayları

Conditioned media of tonsil derived mesenchymal stem cells shows different rates of cytotoxicity on solid cancer cells

Yazarlar:

Melek Yüce | Esra Albayrak | Çağrı Gümüşkaptan

Yayıncı:

MediHealth Academy Yayıncılık

Ana Başlıklar:

Rejeneratif Tıp (kök hücreler dahil)

Dergi:

Anatolian Current Medical Journal Vol.5 Issue 4, pp.503-510

Belge Tipi:

Makale

Yayın Tarihi:

10/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001302561

pISSN:

2718-0115

Anahtar kelimeler:

Özet:

 Aims: Mesenchymal stem cells MSCs are the apple of the eye of cancer studies. It was indicated that the secreted factors, especially released by MSCs, have tumoral or anti-tumoral effects on tumor progression. MSCs obtained from different sources show different anti-tumoral effects, while MSCs originating from the same source also show different tumoral effects in different cancer cells. Here, we investigated the anti-tumor effects of soluble factors secreted from palatine tonsil MSCs TMSC as a new source of MSC on human lung carcinoma A549 and pancreatic cancer PANC-1 cell lines. Methods: Conditioned medium CM was obtained from TMSCs isolated from palatine tonsil tissue, and the cytotoxic effect of CM on the growth of A549 and PANC-1 in a dose-dependent manner was demonstrated by MTT analysis. In addition, the function of CM treatment on the cell cycle status of cancer cells and the apoptosis process were investigated through cell cycle analysis with propidium iodide PI and Annexin-VPI detection method by flow cytometry analysis, respectively. Results: We demonstrated that TMSC-CM treatment significantly decreased the viability of A549 and PANC-1 cell lines in a dose-dependent manner post-48 hours. In addition, CM treatment differentially induced the apoptosis on A549 and PANC-1 cells and also, caused G2M arrest in the cells. Conclusion: In light of these findings, our study is the first to show that TMSC-CM has an anti-tumoral effect by stimulating apoptosis on A549 and PANC-1 cells. These findings reveal that the usage of CM has a cell-free cellular therapeutic potential.

 Aims: Mesenchymal stem cells MSCs are the apple of the eye of cancer studies. It was indicated that the secreted factors, especially released by MSCs, have tumoral or anti-tumoral effects on tumor progression. MSCs obtained from different sources show different anti-tumoral effects, while MSCs originating from the same source also show different tumoral effects in different cancer cells. Here, we investigated the anti-tumor effects of soluble factors secreted from palatine tonsil MSCs TMSC as a new source of MSC on human lung carcinoma A549 and pancreatic cancer PANC-1 cell lines. Methods: Conditioned medium CM was obtained from TMSCs isolated from palatine tonsil tissue, and the cytotoxic effect of CM on the growth of A549 and PANC-1 in a dose-dependent manner was demonstrated by MTT analysis. In addition, the function of CM treatment on the cell cycle status of cancer cells and the apoptosis process were investigated through cell cycle analysis with propidium iodide PI and Annexin-VPI detection method by flow cytometry analysis, respectively. Results: We demonstrated that TMSC-CM treatment significantly decreased the viability of A549 and PANC-1 cell lines in a dose-dependent manner post-48 hours. In addition, CM treatment differentially induced the apoptosis on A549 and PANC-1 cells and also, caused G2M arrest in the cells. Conclusion: In light of these findings, our study is the first to show that TMSC-CM has an anti-tumoral effect by stimulating apoptosis on A549 and PANC-1 cells. These findings reveal that the usage of CM has a cell-free cellular therapeutic potential.

 Aims: Mesenchymal stem cells MSCs are the apple of the eye of cancer studies. It was indicated that the secreted factors, especially released by MSCs, have tumoral or anti-tumoral effects on tumor progression. MSCs obtained from different sources show different anti-tumoral effects, while MSCs originating from the same source also show different tumoral effects in different cancer cells. Here, we investigated the anti-tumor effects of soluble factors secreted from palatine tonsil MSCs TMSC as a new source of MSC on human lung carcinoma A549 and pancreatic cancer PANC-1 cell lines. Methods: Conditioned medium CM was obtained from TMSCs isolated from palatine tonsil tissue, and the cytotoxic effect of CM on the growth of A549 and PANC-1 in a dose-dependent manner was demonstrated by MTT analysis. In addition, the function of CM treatment on the cell cycle status of cancer cells and the apoptosis process were investigated through cell cycle analysis with propidium iodide PI and Annexin-VPI detection method by flow cytometry analysis, respectively. Results: We demonstrated that TMSC-CM treatment significantly decreased the viability of A549 and PANC-1 cell lines in a dose-dependent manner post-48 hours. In addition, CM treatment differentially induced the apoptosis on A549 and PANC-1 cells and also, caused G2M arrest in the cells. Conclusion: In light of these findings, our study is the first to show that TMSC-CM has an anti-tumoral effect by stimulating apoptosis on A549 and PANC-1 cells. These findings reveal that the usage of CM has a cell-free cellular therapeutic potential.

 Aims: Mesenchymal stem cells MSCs are the apple of the eye of cancer studies. It was indicated that the secreted factors, especially released by MSCs, have tumoral or anti-tumoral effects on tumor progression. MSCs obtained from different sources show different anti-tumoral effects, while MSCs originating from the same source also show different tumoral effects in different cancer cells. Here, we investigated the anti-tumor effects of soluble factors secreted from palatine tonsil MSCs TMSC as a new source of MSC on human lung carcinoma A549 and pancreatic cancer PANC-1 cell lines. Methods: Conditioned medium CM was obtained from TMSCs isolated from palatine tonsil tissue, and the cytotoxic effect of CM on the growth of A549 and PANC-1 in a dose-dependent manner was demonstrated by MTT analysis. In addition, the function of CM treatment on the cell cycle status of cancer cells and the apoptosis process were investigated through cell cycle analysis with propidium iodide PI and Annexin-VPI detection method by flow cytometry analysis, respectively. Results: We demonstrated that TMSC-CM treatment significantly decreased the viability of A549 and PANC-1 cell lines in a dose-dependent manner post-48 hours. In addition, CM treatment differentially induced the apoptosis on A549 and PANC-1 cells and also, caused G2M arrest in the cells. Conclusion: In light of these findings, our study is the first to show that TMSC-CM has an anti-tumoral effect by stimulating apoptosis on A549 and PANC-1 cells. These findings reveal that the usage of CM has a cell-free cellular therapeutic potential.

Makale detayları

Conditioned media of tonsil derived mesenchymal stem cells shows different rates of cytotoxicity on solid cancer cells

Yazarlar:

Melek Yüce | Esra Albayrak | Çağrı Gümüşkaptan

Yayıncı:

MediHealth Academy Yayıncılık

Ana Başlıklar:

Rejeneratif Tıp (kök hücreler dahil)

Dergi:

Anatolian Current Medical Journal Vol.5 Issue 4, pp.503-510

Belge Tipi:

Makale

Yayın Tarihi:

10/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001302561

pISSN:

2718-0115

Cancer

cellular therapy

cytotoxicity

mesenchymal stem cell

palatine tonsil.

Sayı

Makale detayları

Conditioned media of tonsil derived mesenchymal stem cells shows different rates of cytotoxicity on solid cancer cells

Yazarlar: Melek Yüce | Esra Albayrak | Çağrı Gümüşkaptan

Yayıncı: MediHealth Academy Yayıncılık

Ana Başlıklar: Rejeneratif Tıp (kök hücreler dahil)

Dergi: Anatolian Current Medical Journal Vol.5 Issue 4, pp.503-510

Belge Tipi: Makale

Yayın Tarihi: 10/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001302561

pISSN: 2718-0115

Cancer

cellular therapy

cytotoxicity

mesenchymal stem cell

palatine tonsil.

Sayı

Yazarlar:

Melek Yüce | Esra Albayrak | Çağrı Gümüşkaptan

Yayıncı:

MediHealth Academy Yayıncılık

Ana Başlıklar:

Rejeneratif Tıp (kök hücreler dahil)

Dergi:

Anatolian Current Medical Journal Vol.5 Issue 4, pp.503-510

Belge Tipi:

Makale

Yayın Tarihi:

10/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001302561

pISSN:

2718-0115