CHEMICAL COMPOSITION AND BIOLOGICAL ACTIVITY OF PHOLIOTA AURIVELLA (BATSCH) P. KUMM.

Cenker Yaman; Gizem Gül; Atakan Benek; Dilay Turu; Selime Deniz Bozkurt; Ilgaz Akata; Kerem Canlı

Makale detayları

CHEMICAL COMPOSITION AND BIOLOGICAL ACTIVITY OF PHOLIOTA AURIVELLA (BATSCH) P. KUMM.

Yazarlar:

Cenker Yaman | Gizem Gül | Atakan Benek | Dilay Turu | Selime Deniz Bozkurt | Ilgaz Akata | Kerem Canlı

Yayıncı:

Ankara University

Ana Başlıklar:

Farmasotik Mikrobiyoloji

Dergi:

Ankara Üniversitesi Eczacılık Fakültesi Dergisi Vol.49 Issue 2, pp.349-364

Belge Tipi:

Makale

Yayın Tarihi:

5/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001347676

pISSN:

1015-3918

Anahtar kelimeler:

Özet:

 Objective: This study aims to determine the chemical composition of the mushroom Pholiota aurivella by investigating its antimicrobial, antibiofilm, and antioxidant activities. The potential effects of the ethanol extract of the mushroom on a total of 27 different microorganisms, including strains with multidrug resistance MDR, were examined. Additionally, the biofilm inhibition capacity and free radical scavenging activities of the mushroom extract were tested. Material and Method: The ethanol extract obtained from the P. aurivella sample was subjected to various tests to evaluate its biological activities. Antimicrobial activity was analyzed using disk diffusion and Minimum Inhibitory Concentration MIC tests. Antibiofilm activity was assessed based on its capacity to inhibit biofilm formation, while antioxidant activity was tested using the DPPH method. The chemical composition was determined by Gas Chromatography-Mass Spectrometry GC-MS analysis. Result and Discussion: Our study reveals that P. aurivella is a promising natural agent, especially in terms of its antimicrobial and antibiofilm activities. The extract exhibited antimicrobial activity against 16 different Gram-positive and Gram-negative microorganisms. Expanding zones of inhibition were observed with increasing extract amounts in strains such as Klebsiella pneumoniae and Enterobacter aerogenes, which show multidrug resistance MDR. In biofilm inhibition tests, the strongest effects were observed in strains of Listeria innocua and Bacillus subtilis DSMZ 1971. On the other hand, the antioxidant activity tested by the DPPH method was quite low; this can be explained by the absence of phenolic compounds in the chemical composition of the extract. GC-MS analysis identified the major components as linoleic acid 59.20 and ethyl linoleate 17.13. These findings indicate that P. aurivella, with its antibiofilm and antimicrobial properties, has potential for pharmaceutical applications and may offer an important natural resource for developing treatment options even against MDR pathogens.

 Objective: This study aims to determine the chemical composition of the mushroom Pholiota aurivella by investigating its antimicrobial, antibiofilm, and antioxidant activities. The potential effects of the ethanol extract of the mushroom on a total of 27 different microorganisms, including strains with multidrug resistance MDR, were examined. Additionally, the biofilm inhibition capacity and free radical scavenging activities of the mushroom extract were tested. Material and Method: The ethanol extract obtained from the P. aurivella sample was subjected to various tests to evaluate its biological activities. Antimicrobial activity was analyzed using disk diffusion and Minimum Inhibitory Concentration MIC tests. Antibiofilm activity was assessed based on its capacity to inhibit biofilm formation, while antioxidant activity was tested using the DPPH method. The chemical composition was determined by Gas Chromatography-Mass Spectrometry GC-MS analysis. Result and Discussion: Our study reveals that P. aurivella is a promising natural agent, especially in terms of its antimicrobial and antibiofilm activities. The extract exhibited antimicrobial activity against 16 different Gram-positive and Gram-negative microorganisms. Expanding zones of inhibition were observed with increasing extract amounts in strains such as Klebsiella pneumoniae and Enterobacter aerogenes, which show multidrug resistance MDR. In biofilm inhibition tests, the strongest effects were observed in strains of Listeria innocua and Bacillus subtilis DSMZ 1971. On the other hand, the antioxidant activity tested by the DPPH method was quite low; this can be explained by the absence of phenolic compounds in the chemical composition of the extract. GC-MS analysis identified the major components as linoleic acid 59.20 and ethyl linoleate 17.13. These findings indicate that P. aurivella, with its antibiofilm and antimicrobial properties, has potential for pharmaceutical applications and may offer an important natural resource for developing treatment options even against MDR pathogens.

 Objective: This study aims to determine the chemical composition of the mushroom Pholiota aurivella by investigating its antimicrobial, antibiofilm, and antioxidant activities. The potential effects of the ethanol extract of the mushroom on a total of 27 different microorganisms, including strains with multidrug resistance MDR, were examined. Additionally, the biofilm inhibition capacity and free radical scavenging activities of the mushroom extract were tested. Material and Method: The ethanol extract obtained from the P. aurivella sample was subjected to various tests to evaluate its biological activities. Antimicrobial activity was analyzed using disk diffusion and Minimum Inhibitory Concentration MIC tests. Antibiofilm activity was assessed based on its capacity to inhibit biofilm formation, while antioxidant activity was tested using the DPPH method. The chemical composition was determined by Gas Chromatography-Mass Spectrometry GC-MS analysis. Result and Discussion: Our study reveals that P. aurivella is a promising natural agent, especially in terms of its antimicrobial and antibiofilm activities. The extract exhibited antimicrobial activity against 16 different Gram-positive and Gram-negative microorganisms. Expanding zones of inhibition were observed with increasing extract amounts in strains such as Klebsiella pneumoniae and Enterobacter aerogenes, which show multidrug resistance MDR. In biofilm inhibition tests, the strongest effects were observed in strains of Listeria innocua and Bacillus subtilis DSMZ 1971. On the other hand, the antioxidant activity tested by the DPPH method was quite low; this can be explained by the absence of phenolic compounds in the chemical composition of the extract. GC-MS analysis identified the major components as linoleic acid 59.20 and ethyl linoleate 17.13. These findings indicate that P. aurivella, with its antibiofilm and antimicrobial properties, has potential for pharmaceutical applications and may offer an important natural resource for developing treatment options even against MDR pathogens.

 Amaç: Bu çalışma, Pholiota aurivella mantarının antimikrobiyal, antibiyofilm ve antioksidan aktivitelerini araştırarak kimyasal kompozisyonunu belirlemeyi amaçlamaktadır. Çalışmada, mantarın etanol ekstraktının, çoklu ilaç direncine sahip suşlar da dahil olmak üzere toplam 27 farklı mikroorganizma üzerindeki potansiyel etkisi incelenmiştir. Ayrıca, mantar ekstraktının biyofilm inhibisyon kapasitesi ve serbest radikal süpürme aktiviteleri test edilmiştir. Gereç ve Yöntem: P. aurivella örneğinden etanol ile elde edilen ekstrakt, biyolojik aktivitelerini değerlendirmek amacıyla çeşitli testlere tabi tutulmuştur. Antimikrobiyal etkinlik, disk difüzyon ve Minimum İnhibitör Konsantrasyon MİK testleri ile analiz edilmiştir. Antibiyofilm aktivitesi, biyofilm oluşumunu inhibe etme kapasitesiyle değerlendirilirken, antioksidan aktivite DPPH yöntemi ile test edilmiştir. Kimyasal kompozisyon ise Gaz Kromatografisi-Kütle Spektrometrisi GC-MS analiziyle belirlenmiştir. Sonuç ve Tartışma: Çalışmamız, P. aurivellanın özellikle antimikrobiyal ve antibiyofilm aktiviteleri açısından umut verici bir doğal ajan olduğunu ortaya koymaktadır. Ekstrakt, 16 farklı Gram-pozitif ve Gram-negatif mikroorganizmaya karşı antimikrobiyal etkinlik gösterirken, çoklu ilaç direnci MDR gösteren Klebsiella pneumoniae ve Enterobacter aerogenes gibi suşlarda da artan ekstrakt miktarı ile genişleyen inhibisyon alanları gözlemlenmiştir. Biyofilm inhibisyonu testlerinde de en güçlü etkiler, Listeria innocua ve Bacillus subtilis DSMZ 1971 suşlarında gözlemlenmiştir. Öte yandan, DPPH yöntemiyle test edilen antioksidan aktivite oldukça düşüktür; bu durum, ekstraktın kimyasal bileşiminde fenolik bileşiklerin bulunmamasıyla açıklanabilir. GC-MS analizinde, majör bileşenlerin linoleik asit 59.20 ve etil linoleat 17.13 olduğu tespit edilmiştir. Bu bulgular, P. aurivellanın antibiyofilm ve antimikrobiyal özellikleriyle farmasötik uygulamalar açısından potansiyele sahip olduğunu ve MDR patojenlere karşı dahi tedavi seçenekleri geliştirilmesinde önemli bir doğal kaynak sunabileceğini göstermektedir.

Makale detayları

CHEMICAL COMPOSITION AND BIOLOGICAL ACTIVITY OF PHOLIOTA AURIVELLA (BATSCH) P. KUMM.

Yazarlar:

Cenker Yaman | Gizem Gül | Atakan Benek | Dilay Turu | Selime Deniz Bozkurt | Ilgaz Akata | Kerem Canlı

Yayıncı:

Ankara University

Ana Başlıklar:

Farmasotik Mikrobiyoloji

Dergi:

Ankara Üniversitesi Eczacılık Fakültesi Dergisi Vol.49 Issue 2, pp.349-364

Belge Tipi:

Makale

Yayın Tarihi:

5/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001347676

pISSN:

1015-3918

Antibiofilm activity

antimicrobial activities

antioxidant activity

GC-MS

Pholiota aurivellaAntibyiofilm aktivite

antimikrobiyal aktivite

antioksidan aktivite

GC-MS

Pholiota aurivella

Sayı

Makale detayları

Yazarlar: Cenker Yaman | Gizem Gül | Atakan Benek | Dilay Turu | Selime Deniz Bozkurt | Ilgaz Akata | Kerem Canlı

Yayıncı: Ankara University

Ana Başlıklar: Farmasotik Mikrobiyoloji

Dergi: Ankara Üniversitesi Eczacılık Fakültesi Dergisi Vol.49 Issue 2, pp.349-364

Belge Tipi: Makale

Yayın Tarihi: 5/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001347676

pISSN: 1015-3918

Sayı

Yazarlar:

Cenker Yaman | Gizem Gül | Atakan Benek | Dilay Turu | Selime Deniz Bozkurt | Ilgaz Akata | Kerem Canlı

Yayıncı:

Ankara University

Ana Başlıklar:

Farmasotik Mikrobiyoloji

Dergi:

Ankara Üniversitesi Eczacılık Fakültesi Dergisi Vol.49 Issue 2, pp.349-364

Belge Tipi:

Makale

Yayın Tarihi:

5/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001347676

pISSN:

1015-3918