Interleukin 12B mRNA level and rs3212227 genotyping in peripheral blood  mononuclear cells of inflammatory bowel disease patients

Hamid Asadzadeh AGHDAEI; Elnaz EMAMI; Mahyar NOURIAN; Vahid CHALESHI; Peyman TARBAN; Hedieh BALAII; Pedram AZIMZADEH; Shiva IRANI; Shabnam SHAHROKH; Mohammad ROSTAMI-NEJAD; Mohammad Reza ZALI

Makale detayları

Interleukin 12B mRNA level and rs3212227 genotyping in peripheral blood mononuclear cells of inflammatory bowel disease patients

Yazarlar:

Hamid Asadzadeh AGHDAEI | Elnaz EMAMI | Mahyar NOURIAN | Vahid CHALESHI | Peyman TARBAN | Hedieh BALAII | Pedram AZIMZADEH | Shiva IRANI | Shabnam SHAHROKH | Mohammad ROSTAMI-NEJAD | Mohammad Reza ZALI

Yayıncı:

TUBITAK

Ana Başlıklar:

Sağlık teşvikleri

Dergi:

Turkish Journal of Medical Sciences 2018 Vol.48 Issue 6, pp.1147-1152

Belge Tipi:

Makale

Yayın Tarihi:

12/2018

Belge Dili:

İngilizce

Document ID (Org No.):

2000750449

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

Özet:

 


 
                         Background/aim:
 Inflammatory bowel disease (IBD) is a multifactorial disorder. Single nucleotide polymorphisms (SNPs) in the IL-12 
gene, which are the main factors to regulate the immune reaction, play an important role in the production of IL-12 molecules. The aim 
of this study was to evaluate the correlation between the SNP on position +1188 of the 3
'
UTR region of the IL-12 p40 subunit gene and 
expression of the IL-12 p40 gene.
Materials and methods:
 This case-control study was performed with 102 patients with IBD and 107 healthy people. PCR-RFLP and 
comparative real-time PCR were performed to assess the association between genotype and IL-12 gene expression.
Results:
 The frequency of AA, CA, and CC genotypes of this gene at position +1188 was calculated to be 58.8%, 32.4%, and 8.8% in 
patients  and  61.7%,  26.2%,  and  12.1%  in  the  control  group,  respectively,  with  no  significant  difference  between  the  two  groups  (IL-
12B rs3212227: AA (Reference 1), CA (P = 0.407); OR (95% CI) 0.771 (0.418-1.424), CC (P = 0.561); OR (95% CI) 1.313 (0.524-3.292)). 
Also,  the  IL-12B  mRNA  expression  level  was  compared  between  IBD  patients  and  healthy  controls  and  demonstrated  a  significant  
association (R
2
 0.136, 95% CI 1.892-3.872, P < 0.0001).
Conclusion:
 Our results show that IL-12B expression in IBD may be associated with altered immune and inflammatory responses.
                    

.

Makale detayları

Interleukin 12B mRNA level and rs3212227 genotyping in peripheral blood mononuclear cells of inflammatory bowel disease patients

Yazarlar: Hamid Asadzadeh AGHDAEI | Elnaz EMAMI | Mahyar NOURIAN | Vahid CHALESHI | Peyman TARBAN | Hedieh BALAII | Pedram AZIMZADEH | Shiva IRANI | Shabnam SHAHROKH | Mohammad ROSTAMI-NEJAD | Mohammad Reza ZALI

Yayıncı: TUBITAK

Ana Başlıklar: Sağlık teşvikleri

Dergi: Turkish Journal of Medical Sciences 2018 Vol.48 Issue 6, pp.1147-1152

Belge Tipi: Makale

Yayın Tarihi: 12/2018

Belge Dili: İngilizce

Document ID (Org No.): 2000750449

Sıklık: İki Aylık

eISSN: 1303-6165

pISSN: 1300-0144

Sayı

Yazarlar:

Hamid Asadzadeh AGHDAEI | Elnaz EMAMI | Mahyar NOURIAN | Vahid CHALESHI | Peyman TARBAN | Hedieh BALAII | Pedram AZIMZADEH | Shiva IRANI | Shabnam SHAHROKH | Mohammad ROSTAMI-NEJAD | Mohammad Reza ZALI

Yayıncı:

TUBITAK

Ana Başlıklar:

Sağlık teşvikleri

Dergi:

Turkish Journal of Medical Sciences 2018 Vol.48 Issue 6, pp.1147-1152

Belge Tipi:

Makale

Yayın Tarihi:

12/2018

Belge Dili:

İngilizce

Document ID (Org No.):

2000750449

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144