Amoebicidal and Cytotoxic Activity of Propolis collected from Different Regions in Turkey on Acanthamoeba castellanii Trophozoites

Zeynep Koloren; Ömer Ertürk; Zülal Atlı Şekeroğlu; Ülkü Karaman

Makale detayları

Amoebicidal and Cytotoxic Activity of Propolis collected from Different Regions in Turkey on Acanthamoeba castellanii Trophozoites

Yazarlar:

Zeynep Koloren | Ömer Ertürk | Zülal Atlı Şekeroğlu | Ülkü Karaman

Yayıncı:

Ordu University

Ana Başlıklar:

Health Care Administration

Dergi:

Middle Black Sea Journal of Health Science Vol.9 Issue 2, pp.312-324

Belge Tipi:

Makale

Yayın Tarihi:

5/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001290765

pISSN:

2149-7796

Anahtar kelimeler:

Özet:

 Objective: The present study aimed to investigate the amoebicidal effects of propolis collected from different regions in Turkey on Acanthamoeba trophozoites. Method: The propolis was collected from different geographic sites Van, Erzurum, Gümüşhane, Ordu, Rize, and Muğla in Turkey. Different concentrations of propolis ethanolic extract in quantities from 1, 2, 3, 4, 5, 6, 7, and 8 mgmL and the same volume of trophozoites in 100 μl culture were blended for the identification of the amoebicidal efficiency of propolis. Results: The growth of trophozoites stopped in Turkish propolis extracts with 50 inhibitory concentrations IC5048h for 5 mgmL extract solution. Propolis showed more potent inhibitory effects on Acanthamoeba trophozoites at concentrations of 7, 6, 5, 4, and 3 mgmL for 72 h. Propolis extract substantially inhibited human bronchial epithelial cells, especially at higher concentrations 7, 8, and 16 mgmL. Propolis can kill Acanthamoeba trophozoites at a concentration of 3-6 mgmL but is safe for human bronchial epithelial cells at the same concentrations after 72 h treatment, this paves the way for propolis to be an alternative source of therapeutic drugs in the treatment of Acanthamoeba spp. Conclusion: Acanthamoeba infection still cannot be treated with drugs. In this study, propolis collected from different regions of Turkey showed amoebic and cytotoxic activities. Propolis extract, which is a natural product that can be used against Acanthamoeba trophozoites can be an alternative source of therapeutic drugs in the treatment of Acanthamoeba, supported by in vivo studies.

 Objective: The present study aimed to investigate the amoebicidal effects of propolis collected from different regions in Turkey on Acanthamoeba trophozoites. Method: The propolis was collected from different geographic sites Van, Erzurum, Gümüşhane, Ordu, Rize, and Muğla in Turkey. Different concentrations of propolis ethanolic extract in quantities from 1, 2, 3, 4, 5, 6, 7, and 8 mgmL and the same volume of trophozoites in 100 μl culture were blended for the identification of the amoebicidal efficiency of propolis. Results: The growth of trophozoites stopped in Turkish propolis extracts with 50 inhibitory concentrations IC5048h for 5 mgmL extract solution. Propolis showed more potent inhibitory effects on Acanthamoeba trophozoites at concentrations of 7, 6, 5, 4, and 3 mgmL for 72 h. Propolis extract substantially inhibited human bronchial epithelial cells, especially at higher concentrations 7, 8, and 16 mgmL. Propolis can kill Acanthamoeba trophozoites at a concentration of 3-6 mgmL but is safe for human bronchial epithelial cells at the same concentrations after 72 h treatment, this paves the way for propolis to be an alternative source of therapeutic drugs in the treatment of Acanthamoeba spp. Conclusion: Acanthamoeba infection still cannot be treated with drugs. In this study, propolis collected from different regions of Turkey showed amoebic and cytotoxic activities. Propolis extract, which is a natural product that can be used against Acanthamoeba trophozoites can be an alternative source of therapeutic drugs in the treatment of Acanthamoeba, supported by in vivo studies.

 Objective: The present study aimed to investigate the amoebicidal effects of propolis collected from different regions in Turkey on Acanthamoeba trophozoites. Method: The propolis was collected from different geographic sites Van, Erzurum, Gümüşhane, Ordu, Rize, and Muğla in Turkey. Different concentrations of propolis ethanolic extract in quantities from 1, 2, 3, 4, 5, 6, 7, and 8 mgmL and the same volume of trophozoites in 100 μl culture were blended for the identification of the amoebicidal efficiency of propolis. Results: The growth of trophozoites stopped in Turkish propolis extracts with 50 inhibitory concentrations IC5048h for 5 mgmL extract solution. Propolis showed more potent inhibitory effects on Acanthamoeba trophozoites at concentrations of 7, 6, 5, 4, and 3 mgmL for 72 h. Propolis extract substantially inhibited human bronchial epithelial cells, especially at higher concentrations 7, 8, and 16 mgmL. Propolis can kill Acanthamoeba trophozoites at a concentration of 3-6 mgmL but is safe for human bronchial epithelial cells at the same concentrations after 72 h treatment, this paves the way for propolis to be an alternative source of therapeutic drugs in the treatment of Acanthamoeba spp. Conclusion: Acanthamoeba infection still cannot be treated with drugs. In this study, propolis collected from different regions of Turkey showed amoebic and cytotoxic activities. Propolis extract, which is a natural product that can be used against Acanthamoeba trophozoites can be an alternative source of therapeutic drugs in the treatment of Acanthamoeba, supported by in vivo studies.

 Objective: The present study aimed to investigate the amoebicidal effects of propolis collected from different regions in Turkey on Acanthamoeba trophozoites. Method: The propolis was collected from different geographic sites Van, Erzurum, Gümüşhane, Ordu, Rize, and Muğla in Turkey. Different concentrations of propolis ethanolic extract in quantities from 1, 2, 3, 4, 5, 6, 7, and 8 mgmL and the same volume of trophozoites in 100 μl culture were blended for the identification of the amoebicidal efficiency of propolis. Results: The growth of trophozoites stopped in Turkish propolis extracts with 50 inhibitory concentrations IC5048h for 5 mgmL extract solution. Propolis showed more potent inhibitory effects on Acanthamoeba trophozoites at concentrations of 7, 6, 5, 4, and 3 mgmL for 72 h. Propolis extract substantially inhibited human bronchial epithelial cells, especially at higher concentrations 7, 8, and 16 mgmL. Propolis can kill Acanthamoeba trophozoites at a concentration of 3-6 mgmL but is safe for human bronchial epithelial cells at the same concentrations after 72 h treatment, this paves the way for propolis to be an alternative source of therapeutic drugs in the treatment of Acanthamoeba spp. Conclusion: Acanthamoeba infection still cannot be treated with drugs. In this study, propolis collected from different regions of Turkey showed amoebic and cytotoxic activities. Propolis extract, which is a natural product that can be used against Acanthamoeba trophozoites can be an alternative source of therapeutic drugs in the treatment of Acanthamoeba, supported by in vivo studies.

Makale detayları

Amoebicidal and Cytotoxic Activity of Propolis collected from Different Regions in Turkey on Acanthamoeba castellanii Trophozoites

Yazarlar:

Zeynep Koloren | Ömer Ertürk | Zülal Atlı Şekeroğlu | Ülkü Karaman

Yayıncı:

Ordu University

Ana Başlıklar:

Health Care Administration

Dergi:

Middle Black Sea Journal of Health Science Vol.9 Issue 2, pp.312-324

Belge Tipi:

Makale

Yayın Tarihi:

5/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001290765

pISSN:

2149-7796

Acanthamoeba sp.

amoebicidal activity

Turkish propolis

MTT assay

Cytotoxicity

RP-HPLCAcanthamoeba sp.

amoebicidal activity

Turkish propolis

MTT assay

Cytotoxicity

RP-HPLC

Sayı

Makale detayları

Yazarlar: Zeynep Koloren | Ömer Ertürk | Zülal Atlı Şekeroğlu | Ülkü Karaman

Yayıncı: Ordu University

Ana Başlıklar: Health Care Administration

Dergi: Middle Black Sea Journal of Health Science Vol.9 Issue 2, pp.312-324

Belge Tipi: Makale

Yayın Tarihi: 5/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001290765

pISSN: 2149-7796

Sayı

Yazarlar:

Zeynep Koloren | Ömer Ertürk | Zülal Atlı Şekeroğlu | Ülkü Karaman

Yayıncı:

Ordu University

Ana Başlıklar:

Health Care Administration

Dergi:

Middle Black Sea Journal of Health Science Vol.9 Issue 2, pp.312-324

Belge Tipi:

Makale

Yayın Tarihi:

5/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001290765

pISSN:

2149-7796