The Effects on Post-Thaw Sperm Quality and Nuclear DNA Integrity of Supplementation of Low-Density Lipoprotein to Freezing Extender in the Mouse

Cengiz Yıldız

Makale detayları

The Effects on Post-Thaw Sperm Quality and Nuclear DNA Integrity of Supplementation of Low-Density Lipoprotein to Freezing Extender in the Mouse

Yazarlar:

Cengiz Yıldız

Yayıncı:

Van Yuzuncu Yıl University

Ana Başlıklar:

Dölerme ve Suni Tohumlama

Dergi:

Van Veterinary Journal Vol.35 Issue 1, pp.94-100

Belge Tipi:

Makale

Yayın Tarihi:

3/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001314749

pISSN:

2149-3359

Anahtar kelimeler:

Özet:

 Mice are an important research tool for genetic and molecular biology, allowing researchers to explore a variety of human illness models. Egg yolk is a common component of semen extenders for domestic animals and low-density lipoproteins LDL from egg yolk have some cryoprotective properties. This study aimed to investigate sperm quality characteristics and nuclear DNA integrity after post-thawing in an extender 18 raffinose  3 skim milk supplemented with different concentrations of LDL 2.5, 5.0, 7.5, or 10 in mice. 18 Raffinose3 skim milk extender was used as a control group without LDL. CD-1 mice were used in the study, and semen was collected from the cauda epididymis and diluted with the extender. The straws were then frozen and thawed to evaluate progressive motility, viability, plasma membrane HOST, acrosome, and nuclear DNA integrity parameters. Fresh sperm had the highest progressive motility, viability, plasma membrane integrity, and longevity endurance of progressive motility for 4 h in HTF solution. The greatest spermatologic results, including nuclear DNA integrity, were determined in fresh sperm p0.05. The greatest post-thaw progressive motility 56.71.3, viability 11.62.9, membrane integrity 63.02.7, and longevity at the end of the 4 h 31.32.1 were found in the group with supplementation of 2.5 LDL compared with the control group. The lowest progressive motility, viability, and plasma membrane integrity were determined in the 10 LDL group p0.05. The cryopreservation process increased the rates of fragmented DNA in all tested LDL and control groups compared with fresh sperm p0.05. There was no statistical difference between the control and experimental groups in terms of nuclear DNA damage after freezing and thawing p0.05. It was concluded that the addition of 2.5 LDL to the extender improved the spermatological quality parameters after freezing and could be used in the freezing and preservation of mouse sperm as it showed a higher protective effect against cold shock.

 Mice are an important research tool for genetic and molecular biology, allowing researchers to explore a variety of human illness models. Egg yolk is a common component of semen extenders for domestic animals and low-density lipoproteins LDL from egg yolk have some cryoprotective properties. This study aimed to investigate sperm quality characteristics and nuclear DNA integrity after post-thawing in an extender 18 raffinose  3 skim milk supplemented with different concentrations of LDL 2.5, 5.0, 7.5, or 10 in mice. 18 Raffinose3 skim milk extender was used as a control group without LDL. CD-1 mice were used in the study, and semen was collected from the cauda epididymis and diluted with the extender. The straws were then frozen and thawed to evaluate progressive motility, viability, plasma membrane HOST, acrosome, and nuclear DNA integrity parameters. Fresh sperm had the highest progressive motility, viability, plasma membrane integrity, and longevity endurance of progressive motility for 4 h in HTF solution. The greatest spermatologic results, including nuclear DNA integrity, were determined in fresh sperm p0.05. The greatest post-thaw progressive motility 56.71.3, viability 11.62.9, membrane integrity 63.02.7, and longevity at the end of the 4 h 31.32.1 were found in the group with supplementation of 2.5 LDL compared with the control group. The lowest progressive motility, viability, and plasma membrane integrity were determined in the 10 LDL group p0.05. The cryopreservation process increased the rates of fragmented DNA in all tested LDL and control groups compared with fresh sperm p0.05. There was no statistical difference between the control and experimental groups in terms of nuclear DNA damage after freezing and thawing p0.05. It was concluded that the addition of 2.5 LDL to the extender improved the spermatological quality parameters after freezing and could be used in the freezing and preservation of mouse sperm as it showed a higher protective effect against cold shock.

 Mice are an important research tool for genetic and molecular biology, allowing researchers to explore a variety of human illness models. Egg yolk is a common component of semen extenders for domestic animals and low-density lipoproteins LDL from egg yolk have some cryoprotective properties. This study aimed to investigate sperm quality characteristics and nuclear DNA integrity after post-thawing in an extender 18 raffinose  3 skim milk supplemented with different concentrations of LDL 2.5, 5.0, 7.5, or 10 in mice. 18 Raffinose3 skim milk extender was used as a control group without LDL. CD-1 mice were used in the study, and semen was collected from the cauda epididymis and diluted with the extender. The straws were then frozen and thawed to evaluate progressive motility, viability, plasma membrane HOST, acrosome, and nuclear DNA integrity parameters. Fresh sperm had the highest progressive motility, viability, plasma membrane integrity, and longevity endurance of progressive motility for 4 h in HTF solution. The greatest spermatologic results, including nuclear DNA integrity, were determined in fresh sperm p0.05. The greatest post-thaw progressive motility 56.71.3, viability 11.62.9, membrane integrity 63.02.7, and longevity at the end of the 4 h 31.32.1 were found in the group with supplementation of 2.5 LDL compared with the control group. The lowest progressive motility, viability, and plasma membrane integrity were determined in the 10 LDL group p0.05. The cryopreservation process increased the rates of fragmented DNA in all tested LDL and control groups compared with fresh sperm p0.05. There was no statistical difference between the control and experimental groups in terms of nuclear DNA damage after freezing and thawing p0.05. It was concluded that the addition of 2.5 LDL to the extender improved the spermatological quality parameters after freezing and could be used in the freezing and preservation of mouse sperm as it showed a higher protective effect against cold shock.

 Fareler genetik ve moleküler biyoloji için önemli bir araştırma aracıdır ve araştırmacıların çeşitli insan hastalığı modellerini keşfetmesine olanak tanır. Yumurta sarısı, evcil hayvanlara yönelik sperma sulandırıcılarının yaygın bir bileşenidir ve yumurta sarısından elde edilen düşük yoğunluklu lipoproteinlerin LDL bazı kriyoprotektif özellikleri vardır. Bu çalışma, farede farklı konsantrasyonlarda LDL 2.5, 5.0, 7.5, 10 ilaveli sperma sulandırıcısında 18 rafinoz  3 yağsız süt dondurma sonrası sperm kalite özelliklerini ve nükleer DNA bütünlüğünü araştırmayı amaçladı. LDL içermeyen kontrol grubu olarak 18 Raffinoz3 yağsız süt sulandırıcısı kullanıldı. Çalışmada CD-1 fareler kullanıldı ve kauda epididimisten sperma toplandı ve sperma sulandırıcısı ile sulandırıldı, payetlere çekilerek motilite, canlılık, plazma zarı HOST, akrozom ve nükleer DNA bütünlüğü parametreleri dondurma sonrası değerlendirildi. Nativ sperma, HTF çözeltisinde 4 saat boyunca en yüksek motilite, canlılık, plazma zarı bütünlüğüne ve dayanıklılığa sahip bulundu. Nükleer DNA bütünlüğü taze spermada en yüksek belirlendi p0.05. Çözdürme sonrası en yüksek motilite 56.71.3, canlılık 11.62.9, membran bütünlüğü 63.02.7 ve yaşam süresi 31.32.1 2.5 LDL takviyeli grupta, en düşük progresif motilite, canlılık ve plazma membran bütünlüğü ise 10 LDL grubunda belirlendi p0.05. Kriyoprezervasyon işlemi, taze sperm ile karşılaştırıldığında tüm LDL ve kontrol gruplarında parçalanmış DNA oranlarını arttırdı p0.05. Kontrol ve deney grupları arasında donma ve çözdürme sonrası nükleer DNA hasarı açısından istatistiksel fark yoktu p0.05. Sulandırıcıya 2.5 LDL ilavesinin dondurma sonrası spermatolojik kalite parametrelerini iyileştirdiği ve fare sperminin dondurulması ve korunmasında daha yüksek oranda soğuk şokuna karşı koruyucu etki gösterdiği için kullanılabileceği sonucuna varıldı.

Makale detayları

The Effects on Post-Thaw Sperm Quality and Nuclear DNA Integrity of Supplementation of Low-Density Lipoprotein to Freezing Extender in the Mouse

Yazarlar:

Cengiz Yıldız

Yayıncı:

Van Yuzuncu Yıl University

Ana Başlıklar:

Dölerme ve Suni Tohumlama

Dergi:

Van Veterinary Journal Vol.35 Issue 1, pp.94-100

Belge Tipi:

Makale

Yayın Tarihi:

3/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001314749

pISSN:

2149-3359

Mouse sperm

Low Density Lypoprotein

Sperm analysisFare spermi

Düşük Yoğunluklu Lipoprotein

Sperm analizi

Sayı

Makale detayları

The Effects on Post-Thaw Sperm Quality and Nuclear DNA Integrity of Supplementation of Low-Density Lipoprotein to Freezing Extender in the Mouse

Yazarlar: Cengiz Yıldız

Yayıncı: Van Yuzuncu Yıl University

Ana Başlıklar: Dölerme ve Suni Tohumlama

Dergi: Van Veterinary Journal Vol.35 Issue 1, pp.94-100

Belge Tipi: Makale

Yayın Tarihi: 3/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001314749

pISSN: 2149-3359

Mouse sperm

Low Density Lypoprotein

Sperm analysisFare spermi

Düşük Yoğunluklu Lipoprotein

Sperm analizi

Sayı

Yazarlar:

Cengiz Yıldız

Yayıncı:

Van Yuzuncu Yıl University

Ana Başlıklar:

Dölerme ve Suni Tohumlama

Dergi:

Van Veterinary Journal Vol.35 Issue 1, pp.94-100

Belge Tipi:

Makale

Yayın Tarihi:

3/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001314749

pISSN:

2149-3359