Therapeutic potential of morin hydrate in methotrexate-induced liver in experimental rats: regulation of organ function and alleviation of oxidative stress

Tahir Kahraman; Şeyda Seçgin; Muhammad Nasir Bhaya; Mehmet Fatih Bozkurt; Hikmet Keleş; Sarab Hayder Weli Weli; İdris Türel

Makale detayları

Therapeutic potential of morin hydrate in methotrexate-induced liver in experimental rats: regulation of organ function and alleviation of oxidative stress

Yazarlar:

Tahir Kahraman | Şeyda Seçgin | Muhammad Nasir Bhaya | Mehmet Fatih Bozkurt | Hikmet Keleş | Sarab Hayder Weli Weli | İdris Türel

Yayıncı:

Cukurova University

Ana Başlıklar:

Gastroenteroloji ve Hepatoloji | Tıbbi Biyokimya - Lipidler

Dergi:

Cukurova Medical Journal Vol.50 Issue 1, pp.211-220

Belge Tipi:

Makale

Yayın Tarihi:

3/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001343864

pISSN:

2602-3032

Anahtar kelimeler:

Özet:

 Purpose: Methotrexate MTX is an antineoplastic agent with a wide clinical use. However, its complications in tissues cause limitations. The effects of many compounds have been studied in reducing the toxicity of MTX in liver tissue. There are limited studies on the elimination of this damage with morin hydrate MH flavonoid. In this study, we investigated the effect of MH in MTX-induced hepatoxicity with a focus on oxidative stress. Materials and Methods: In the experiment, 32 female rats were grouped as Control, MTX, MH, MTXMH n8. On the first day of the study, MTX 20 mgkg single dose was administered ip. MH was administered 100 mgkg by gavage for 10 days. On the 11th day, biochemical and histopathological analyses were performed on liver tissues from rats. All data were presented with statistical comparison. Results: Serum AST, ALT and LDH levels were highest in the MTX group and lower in the MTXMH group. MH increased glutathione peroxidase GPX, reduced glutathione GSH and total antioxidant capacity TAC levels, but had no regulatory effect on superoxide dismutase SOD level. Additionally, MH significantly lowered malondialdehyde MDA levels and reduced total oxidant capacity TOC. Histopathological findings included inflammation, congestion, degeneration, mononuclear cell clusters and dead cells. Tissue damage was most severe in the MTX group, whereas these effects were attenuated in the MTXMH group. Conclusion: Our results indicated that MH may be a potential therapeutic agent in MTX-induced acute liver injury.

 Purpose: Methotrexate MTX is an antineoplastic agent with a wide clinical use. However, its complications in tissues cause limitations. The effects of many compounds have been studied in reducing the toxicity of MTX in liver tissue. There are limited studies on the elimination of this damage with morin hydrate MH flavonoid. In this study, we investigated the effect of MH in MTX-induced hepatoxicity with a focus on oxidative stress. Materials and Methods: In the experiment, 32 female rats were grouped as Control, MTX, MH, MTXMH n8. On the first day of the study, MTX 20 mgkg single dose was administered ip. MH was administered 100 mgkg by gavage for 10 days. On the 11th day, biochemical and histopathological analyses were performed on liver tissues from rats. All data were presented with statistical comparison. Results: Serum AST, ALT and LDH levels were highest in the MTX group and lower in the MTXMH group. MH increased glutathione peroxidase GPX, reduced glutathione GSH and total antioxidant capacity TAC levels, but had no regulatory effect on superoxide dismutase SOD level. Additionally, MH significantly lowered malondialdehyde MDA levels and reduced total oxidant capacity TOC. Histopathological findings included inflammation, congestion, degeneration, mononuclear cell clusters and dead cells. Tissue damage was most severe in the MTX group, whereas these effects were attenuated in the MTXMH group. Conclusion: Our results indicated that MH may be a potential therapeutic agent in MTX-induced acute liver injury.

 Purpose: Methotrexate MTX is an antineoplastic agent with a wide clinical use. However, its complications in tissues cause limitations. The effects of many compounds have been studied in reducing the toxicity of MTX in liver tissue. There are limited studies on the elimination of this damage with morin hydrate MH flavonoid. In this study, we investigated the effect of MH in MTX-induced hepatoxicity with a focus on oxidative stress. Materials and Methods: In the experiment, 32 female rats were grouped as Control, MTX, MH, MTXMH n8. On the first day of the study, MTX 20 mgkg single dose was administered ip. MH was administered 100 mgkg by gavage for 10 days. On the 11th day, biochemical and histopathological analyses were performed on liver tissues from rats. All data were presented with statistical comparison. Results: Serum AST, ALT and LDH levels were highest in the MTX group and lower in the MTXMH group. MH increased glutathione peroxidase GPX, reduced glutathione GSH and total antioxidant capacity TAC levels, but had no regulatory effect on superoxide dismutase SOD level. Additionally, MH significantly lowered malondialdehyde MDA levels and reduced total oxidant capacity TOC. Histopathological findings included inflammation, congestion, degeneration, mononuclear cell clusters and dead cells. Tissue damage was most severe in the MTX group, whereas these effects were attenuated in the MTXMH group. Conclusion: Our results indicated that MH may be a potential therapeutic agent in MTX-induced acute liver injury.

 Amaç: Metotreksat MTX geniş klinik kullanımı olan antineoplastik bir ajandır. Bununla birlikte, dokulardaki komplikasyonları sınırlamalara neden olmaktadır. MTXin karaciğer dokusundaki toksisitesini azaltmada birçok bileşikle çalışılmıştır. Morin hidrat MH flavonoidi ile bu hasarın giderilmesi konusunda sınırlı sayıda çalışma bulunmaktadır. Bu çalışmada MTX ile indüklenen hepatoksisitede oksidatif strese odaklanılarak MHnin etkisi araştırıldı. Gereç ve Yöntem: Deneyde 32 dişi sıçan Kontrol, MTX, MH, MTXMH olarak gruplandırıldı n8. Çalışmanın ilk gününde MTX 20 mgkg tek doz ip olarak uygulandı. MH 10 gün boyunca 100 mgkg gavaj yoluyla uygulandı. 11. günde sıçanlardan alınan karaciğer dokuları üzerinde biyokimyasal ve histopatolojik analizler yapıldı. Tüm veriler istatistiksel karşılaştırma ile sunulmuştur. Bulgular: Serum AST, ALT ve LDH düzeyleri MTX grubunda en yüksek, MTXMH grubunda ise daha düşüktü. MH glutatyon peroksidaz GPX, indirgenmiş glutatyon GSH ve toplam antioksidan kapasite TAC düzeylerini artırırken, süperoksit dismutaz SOD düzeyi üzerinde düzenleyici bir etkisi olmamıştır. Ayrıca, MH malondialdehit MDA seviyelerini önemli ölçüde düşürmüş ve toplam oksidan kapasiteyi TOC azaltmıştır. Histopatolojik bulgular arasında enflamasyon, konjesyon, dejenerasyon, mononükleer hücre kümeleri ve ölü hücreler yer almıştır. Doku hasarı en şiddetli MTX grubunda görülürken, MTXMH grubunda bu etkiler hafiflemiştir. Sonuç: Sonuçlarımız MHnin MTX ile indüklenen akut karaciğer hasarında potansiyel bir terapötik ajan olabileceğini göstermiştir.

Makale detayları

Therapeutic potential of morin hydrate in methotrexate-induced liver in experimental rats: regulation of organ function and alleviation of oxidative stress

Yazarlar:

Tahir Kahraman | Şeyda Seçgin | Muhammad Nasir Bhaya | Mehmet Fatih Bozkurt | Hikmet Keleş | Sarab Hayder Weli Weli | İdris Türel

Yayıncı:

Cukurova University

Ana Başlıklar:

Gastroenteroloji ve Hepatoloji | Tıbbi Biyokimya - Lipidler

Dergi:

Cukurova Medical Journal Vol.50 Issue 1, pp.211-220

Belge Tipi:

Makale

Yayın Tarihi:

3/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001343864

pISSN:

2602-3032

Antioxidant

Hepatoxicity

Liver Function Test

Methotrexate

Morin Hydrate

Oxidative StressAntioksidan

Hepatoksisite

Karaciğer Fonksiyon Testi

Metotreksat

Morin Hidrat

Oksidatif Stres

Sayı

Makale detayları

Yazarlar: Tahir Kahraman | Şeyda Seçgin | Muhammad Nasir Bhaya | Mehmet Fatih Bozkurt | Hikmet Keleş | Sarab Hayder Weli Weli | İdris Türel

Yayıncı: Cukurova University

Ana Başlıklar: Gastroenteroloji ve Hepatoloji | Tıbbi Biyokimya - Lipidler

Dergi: Cukurova Medical Journal Vol.50 Issue 1, pp.211-220

Belge Tipi: Makale

Yayın Tarihi: 3/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001343864

pISSN: 2602-3032

Sayı

Yazarlar:

Tahir Kahraman | Şeyda Seçgin | Muhammad Nasir Bhaya | Mehmet Fatih Bozkurt | Hikmet Keleş | Sarab Hayder Weli Weli | İdris Türel

Yayıncı:

Cukurova University

Ana Başlıklar:

Gastroenteroloji ve Hepatoloji | Tıbbi Biyokimya - Lipidler

Dergi:

Cukurova Medical Journal Vol.50 Issue 1, pp.211-220

Belge Tipi:

Makale

Yayın Tarihi:

3/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001343864

pISSN:

2602-3032