Polymorphonuclear leukocyte isolation from venous blood of the dog

Güneş KARAKURT; Kader YILDIZ

Makale detayları

Polymorphonuclear leukocyte isolation from venous blood of the dog

Yazarlar:

Güneş KARAKURT | Kader YILDIZ

Yayıncı:

The Chamber of Veterinary Surgeons, Istanbul

Ana Başlıklar:

Hayvancılık

Dergi:

Journal of Istanbul Veterinary Sciences 2020, pp.52-52

Belge Tipi:

Makale

Yayın Tarihi:

3/2020

Belge Dili:

İngilizce

Document ID (Org No.):

2000485313

Sıklık:

Üç Yıllık

eISSN:

2602-3490

pISSN:

2602-3490

Özet:

 

The aim of the current study was to neutrophil isolation from venous blood samples of healthy dogs.
Venous blood samples were obtained from Venae cephalica of clinically healthy dogs (n:5) into
heparinized tubes. The blood samples (2 mL) and Percoll dilutions (45%, 54%, 63%, and 72%) prepared
with Hanks Balanced Salt Solution (HBSS) were layered into sterile tubes. After centrifuge, the
polymorphonuclear leukocytes (PMN) were aspirated between 63% and 72% interfaces of the Percoll
dilutions into tubes. The samples of PMN observed under light microscopy. Viability was detected
microscopically after stained with trypan blue dye. Diff-Quick staining was used to detect neutrophil
purity of the isolated PMN. In the present study, the neutrophils ratio was calculated as 92% of the isolated
polymorphonuclear cells. The neutrophil viability was calculated as 98% of PMNs isolated from the
venous blood samples of healthy dogs. In the present study, the Percoll gradient centrifugation (72%, 63%,
54% and 45%) is fast technic for isolation of the neutrophils from venous blood samples of dogs.

Makale detayları

Polymorphonuclear leukocyte isolation from venous blood of the dog

Yazarlar: Güneş KARAKURT | Kader YILDIZ

Yayıncı: The Chamber of Veterinary Surgeons, Istanbul

Ana Başlıklar: Hayvancılık

Dergi: Journal of Istanbul Veterinary Sciences 2020, pp.52-52

Belge Tipi: Makale

Yayın Tarihi: 3/2020

Belge Dili: İngilizce

Document ID (Org No.): 2000485313

Sıklık: Üç Yıllık

eISSN: 2602-3490

pISSN: 2602-3490

Sayı

Yazarlar:

Güneş KARAKURT | Kader YILDIZ

Yayıncı:

The Chamber of Veterinary Surgeons, Istanbul

Ana Başlıklar:

Hayvancılık

Dergi:

Journal of Istanbul Veterinary Sciences 2020, pp.52-52

Belge Tipi:

Makale

Yayın Tarihi:

3/2020

Belge Dili:

İngilizce

Document ID (Org No.):

2000485313

Sıklık:

Üç Yıllık

eISSN:

2602-3490

pISSN:

2602-3490