Prevalence of blaZ gene and other virulence genes in penicillin-resistant Staphylococcus aureus isolated from bovine mastitis cases in Gansu, China

Feng YANG; Qi WANG; Xurong WANG; Ling WANG; Min XIAO; Xinpu LI; Jinyin LUO; Shidong ZHANG; Hongsheng LI

Makale detayları

Prevalence of blaZ gene and other virulence genes in penicillin-resistant Staphylococcus aureus isolated from bovine mastitis cases in Gansu, China

Yazarlar:

Feng YANG | Qi WANG | Xurong WANG | Ling WANG | Min XIAO | Xinpu LI | Jinyin LUO | Shidong ZHANG | Hongsheng LI

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2015 Vol.39 Issue 5, pp.634-636

Belge Tipi:

Makale

Yayın Tarihi:

12/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000765472

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

Anahtar kelimeler:

Özet:

 

Staphylococcus aureus is a major etiological agent of bovine mastitis worldwide. In this study, 37 strains of S. Aureus resistant to penicillin were isolated from bovine mastitis cases in Gansu Province for investigating blaZ and virulence-related genes, including tst, eta, etb, lukPV, lukED, lukM, hla, hlb, hld, and edin. Antibiotic resistance was based on disk diffusion method and blaZ and virulence-associated genes were studied by polymerase chain reaction. Penicillin resistance gene blaZ was detected in 35/37 (94.6%) of penicillin-resistant S. Aureus isolates. tst, lukPV, lukED, hla, hlb, and hld were observed in 5.4%, 2.7%, 89.2%, 70.3%, 73.0%, and 70.3% of the penicillin-resistant isolates, respectively, while eta, etb, lukM, and edin were not detected in any isolates. blaZ carried by penicillin-resistant S. Aureus isolates may be the main reason for phenotypic penicillin resistance. Virulence determinants encoded by lukED, hla, hlb, and hld genes may play important roles in bovine mastitis pathogenesis of penicillin-resistant S. Aureus in Gansu Province.

 

Staphylococcus aureus is a major etiological agent of bovine mastitis worldwide. In this study, 37 strains of S. Aureus resistant to penicillin were isolated from bovine mastitis cases in Gansu Province for investigating blaZ and virulence-related genes, including tst, eta, etb, lukPV, lukED, lukM, hla, hlb, hld, and edin. Antibiotic resistance was based on disk diffusion method and blaZ and virulence-associated genes were studied by polymerase chain reaction. Penicillin resistance gene blaZ was detected in 35/37 (94.6%) of penicillin-resistant S. Aureus isolates. tst, lukPV, lukED, hla, hlb, and hld were observed in 5.4%, 2.7%, 89.2%, 70.3%, 73.0%, and 70.3% of the penicillin-resistant isolates, respectively, while eta, etb, lukM, and edin were not detected in any isolates. blaZ carried by penicillin-resistant S. Aureus isolates may be the main reason for phenotypic penicillin resistance. Virulence determinants encoded by lukED, hla, hlb, and hld genes may play important roles in bovine mastitis pathogenesis of penicillin-resistant S. Aureus in Gansu Province.

Makale detayları

Prevalence of blaZ gene and other virulence genes in penicillin-resistant Staphylococcus aureus isolated from bovine mastitis cases in Gansu, China

Yazarlar:

Feng YANG | Qi WANG | Xurong WANG | Ling WANG | Min XIAO | Xinpu LI | Jinyin LUO | Shidong ZHANG | Hongsheng LI

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2015 Vol.39 Issue 5, pp.634-636

Belge Tipi:

Makale

Yayın Tarihi:

12/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000765472

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

Bovine mastitis

Staphylococcus aureus

antibiotic resistance

blaZ

virulence-related genes

Sayı

Makale detayları

Prevalence of blaZ gene and other virulence genes in penicillin-resistant Staphylococcus aureus isolated from bovine mastitis cases in Gansu, China

Yazarlar: Feng YANG | Qi WANG | Xurong WANG | Ling WANG | Min XIAO | Xinpu LI | Jinyin LUO | Shidong ZHANG | Hongsheng LI

Yayıncı: TUBITAK

Dergi: Turkish Journal of Veterinary and Animal Sciences 2015 Vol.39 Issue 5, pp.634-636

Belge Tipi: Makale

Yayın Tarihi: 12/2015

Belge Dili: Türkçe

Document ID (Org No.): 2000765472

Sıklık: İki Aylık

eISSN: 1303-6181

pISSN: 1300-0128

Bovine mastitis

Staphylococcus aureus

antibiotic resistance

blaZ

virulence-related genes

Sayı

Yazarlar:

Feng YANG | Qi WANG | Xurong WANG | Ling WANG | Min XIAO | Xinpu LI | Jinyin LUO | Shidong ZHANG | Hongsheng LI

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2015 Vol.39 Issue 5, pp.634-636

Belge Tipi:

Makale

Yayın Tarihi:

12/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000765472

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128