Diagnostic Potential of miR-551b-3p in Lung Cancer: In Vitro and In Silico Experiments

Esen Çakmak; İbrahim Seyfettin Çelik

Makale detayları

Diagnostic Potential of miR-551b-3p in Lung Cancer: In Vitro and In Silico Experiments

Yazarlar:

Esen Çakmak | İbrahim Seyfettin Çelik

Yayıncı:

Manisa Celal Bayar University

Ana Başlıklar:

Biyomedikal Bilimler ve Teknolojiler

Dergi:

Celal Bayar Üniversitesi Fen Bilimleri Dergisi Vol.21 Issue 2, pp.125-133

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001352154

pISSN:

1305-130X

Anahtar kelimeler:

Özet:

 Lung cancer is a leading cause of cancer deaths worldwide. miRNAs have attracted attention as promising biomarkers in lung cancer diagnosis and prognosis. This study investigated the molecular mechanism of miR-551b-3p in lung cancer cells. The gene expression level of miR-551b-3p was investigated using qRT-PCR in healthy and cancerous lung cell lines. The target genes of miR-551b-3p and its function in cancer pathogenesis were also determined by in silico analyses. miR-551b-3p expression was higher in cancer cells compared to healthy lung cells p0.01. The expression level of miR-551b-3p was confirmed in silico in cancerous lung tissue. ERBB4 was identified as a target gene of miR-551b-3p and was down-regulated in cancer cells in comparison to healthy cells. Overexpression of miR-551b-3p and under expression of the ERBB4 gene decreased overall survival in cancer patients. A negative correlation was observed between miR-551b-3p and ERBB4 gene. miR-551b-3p expression was found to be closely associated with clinicopathological factors such as distant metastasis status, lymph node metastasis status and gender. miR-551b-3p target genes were enriched in cancer-related cellular processes. In conclusion, miR-551b-3p may be a potential alternative in treatment strategies as a therapeutic target in lung cancers.

 Lung cancer is a leading cause of cancer deaths worldwide. miRNAs have attracted attention as promising biomarkers in lung cancer diagnosis and prognosis. This study investigated the molecular mechanism of miR-551b-3p in lung cancer cells. The gene expression level of miR-551b-3p was investigated using qRT-PCR in healthy and cancerous lung cell lines. The target genes of miR-551b-3p and its function in cancer pathogenesis were also determined by in silico analyses. miR-551b-3p expression was higher in cancer cells compared to healthy lung cells p0.01. The expression level of miR-551b-3p was confirmed in silico in cancerous lung tissue. ERBB4 was identified as a target gene of miR-551b-3p and was down-regulated in cancer cells in comparison to healthy cells. Overexpression of miR-551b-3p and under expression of the ERBB4 gene decreased overall survival in cancer patients. A negative correlation was observed between miR-551b-3p and ERBB4 gene. miR-551b-3p expression was found to be closely associated with clinicopathological factors such as distant metastasis status, lymph node metastasis status and gender. miR-551b-3p target genes were enriched in cancer-related cellular processes. In conclusion, miR-551b-3p may be a potential alternative in treatment strategies as a therapeutic target in lung cancers.

 Lung cancer is a leading cause of cancer deaths worldwide. miRNAs have attracted attention as promising biomarkers in lung cancer diagnosis and prognosis. This study investigated the molecular mechanism of miR-551b-3p in lung cancer cells. The gene expression level of miR-551b-3p was investigated using qRT-PCR in healthy and cancerous lung cell lines. The target genes of miR-551b-3p and its function in cancer pathogenesis were also determined by in silico analyses. miR-551b-3p expression was higher in cancer cells compared to healthy lung cells p0.01. The expression level of miR-551b-3p was confirmed in silico in cancerous lung tissue. ERBB4 was identified as a target gene of miR-551b-3p and was down-regulated in cancer cells in comparison to healthy cells. Overexpression of miR-551b-3p and under expression of the ERBB4 gene decreased overall survival in cancer patients. A negative correlation was observed between miR-551b-3p and ERBB4 gene. miR-551b-3p expression was found to be closely associated with clinicopathological factors such as distant metastasis status, lymph node metastasis status and gender. miR-551b-3p target genes were enriched in cancer-related cellular processes. In conclusion, miR-551b-3p may be a potential alternative in treatment strategies as a therapeutic target in lung cancers.

 Lung cancer is a leading cause of cancer deaths worldwide. miRNAs have attracted attention as promising biomarkers in lung cancer diagnosis and prognosis. This study investigated the molecular mechanism of miR-551b-3p in lung cancer cells. The gene expression level of miR-551b-3p was investigated using qRT-PCR in healthy and cancerous lung cell lines. The target genes of miR-551b-3p and its function in cancer pathogenesis were also determined by in silico analyses. miR-551b-3p expression was higher in cancer cells compared to healthy lung cells p0.01. The expression level of miR-551b-3p was confirmed in silico in cancerous lung tissue. ERBB4 was identified as a target gene of miR-551b-3p and was down-regulated in cancer cells in comparison to healthy cells. Overexpression of miR-551b-3p and under expression of the ERBB4 gene decreased overall survival in cancer patients. A negative correlation was observed between miR-551b-3p and ERBB4 gene. miR-551b-3p expression was found to be closely associated with clinicopathological factors such as distant metastasis status, lymph node metastasis status and gender. miR-551b-3p target genes were enriched in cancer-related cellular processes. In conclusion, miR-551b-3p may be a potential alternative in treatment strategies as a therapeutic target in lung cancers.

Makale detayları

Diagnostic Potential of miR-551b-3p in Lung Cancer: In Vitro and In Silico Experiments

Yazarlar: Esen Çakmak | İbrahim Seyfettin Çelik

Yayıncı: Manisa Celal Bayar University

Ana Başlıklar: Biyomedikal Bilimler ve Teknolojiler

Dergi: Celal Bayar Üniversitesi Fen Bilimleri Dergisi Vol.21 Issue 2, pp.125-133

Belge Tipi: Makale

Yayın Tarihi: 6/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001352154

pISSN: 1305-130X

ERBB4

Lung cancer

miR-551b-3p

qRT-PCR

Sayı

Yazarlar:

Esen Çakmak | İbrahim Seyfettin Çelik

Yayıncı:

Manisa Celal Bayar University

Ana Başlıklar:

Biyomedikal Bilimler ve Teknolojiler

Dergi:

Celal Bayar Üniversitesi Fen Bilimleri Dergisi Vol.21 Issue 2, pp.125-133

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001352154

pISSN:

1305-130X