A Validated HPLC Method for Separation and Determination of Mefloquine Enantiomers in Pharmaceutical Formulations

Y ABOUL-ENEIN; Ola SALEH; Aida EL-AZZOUNI; Amr BADAWEY

Makale detayları

A Validated HPLC Method for Separation and Determination of Mefloquine Enantiomers in Pharmaceutical Formulations

Yazarlar:

Y ABOUL-ENEIN | Ola SALEH | Aida EL-AZZOUNI | Amr BADAWEY

Yayıncı:

Gazi University

Dergi:

Gazi University Journal of Science 2012 Vol.25 Issue 2, pp.377-383

Belge Tipi:

Makale

Yayın Tarihi:

4/2012

Belge Dili:

İngilizce

Document ID (Org No.):

2000429343

eISSN:

2147-1762

Özet:

 


A simple, rapid and validated method for separation and determination of mefloquine enantiomers was developed. Mefolquine was separated and quantitated on cyclodextrin chiral column Quest-CM carboxymethyl-BCD (250x4mm i.d., 5µm particle size) using a mixture of acetonitrile: 1% triethylammonium acetate buffer (pH = 4.5) (20:80 v∕ v) as a mobile phase at 20 oC and a flow rate of 1 mL /min. The UV-detector was set at 240   nm. The applied HPLC method allowed the separation and quantification of mefloquine enantiomers with good linearity (r < 0. 999) in the studied range. The relative standard deviations (RSD) were 0.865 and 0.907 for the mefloquine enantiomers with accuracy of 100.00 and 100.68. The limit of detection and limit of quantification of mefloquine enantionmers were found to be 5 and 15 µg/mL, respectively. The method was validated through the parameters of linearity, accuracy, precision and robustness. The HPLC method was applied for the quantitative determination of mefloquine in pharmaceutical formulations.

 

Keywords  Chiral separation, antimalarials, mefloquine, pharmaceutical analysis.

 

 

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Makale detayları

A Validated HPLC Method for Separation and Determination of Mefloquine Enantiomers in Pharmaceutical Formulations

Yazarlar: Y ABOUL-ENEIN | Ola SALEH | Aida EL-AZZOUNI | Amr BADAWEY

Yayıncı: Gazi University

Dergi: Gazi University Journal of Science 2012 Vol.25 Issue 2, pp.377-383

Belge Tipi: Makale

Yayın Tarihi: 4/2012

Belge Dili: İngilizce

Document ID (Org No.): 2000429343

eISSN: 2147-1762

Sayı

Yazarlar:

Y ABOUL-ENEIN | Ola SALEH | Aida EL-AZZOUNI | Amr BADAWEY

Yayıncı:

Gazi University

Dergi:

Gazi University Journal of Science 2012 Vol.25 Issue 2, pp.377-383

Belge Tipi:

Makale

Yayın Tarihi:

4/2012

Belge Dili:

İngilizce

Document ID (Org No.):

2000429343

eISSN:

2147-1762