A new and rapid HPLC method for the determination of phenoxyethanol in topical formulation

Gamze Ergin Kızılçay; Sıdıka Toker; Dilek Matur

Makale detayları

A new and rapid HPLC method for the determination of phenoxyethanol in topical formulation

Yazarlar:

Gamze Ergin Kızılçay | Sıdıka Toker | Dilek Matur

Yayıncı:

Istanbul University

Ana Başlıklar:

Pharmaceutical Analytical Chemistry

Dergi:

İstanbul Journal of Pharmacy Vol.54 Issue 1, pp.96-101

Belge Tipi:

Makale

Yayın Tarihi:

4/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001318876

pISSN:

2587-2087

Anahtar kelimeler:

Özet:

 Background and Aims: This study develops and validates a new liquid chromatographic method for determining the phenoxyethanol used as an antimicrobial preservative in pharmaceutic and cosmetic products. The study applies the developed high-performance liquid chromatography HPLC method to pomade formulation using a diode array detector to determine the phenoxyethanol. Methods: The phenoxyethanol in the sample was analyzed in a C18 column 150 x 4.6 mm, 5 µm ID under chromatographic conditions where the flow rate was determined as 1.0 mLmin. The column oven was 30.0C, and phenoxyethanol was detected at 270 nm. Isocratic application of acetonitrile-water 50:50, vv was used as the mobile phase system. The validation of the developed method was performed according to the guidelines from the International Conference on Harmonisation ICH, 2005 Guidelines Q2 R1. Results: The linearity range of the phenoxyethanol was 0.125-0.375 mgmL, and the limits of detection and quantification were calculated as 31.25 ngmL and 125.0 ngmL, respectively. The assay recovery and precision of the phenoxyethanol from the pomade formulation were evaluated at 0.125 mgmL, 0.250 mgmL, and 0.375 mgmL concentrations. The mean recoveries for phenoxyethanol in the pomade formulation were calculated at 99.99-102.86. Conclusion: The validated method was successfully applied for determining phenoxyethanol in a topical formulation. The proposed method is cheap, fast, and simple and can be used safely for routine analysis.

 Background and Aims: This study develops and validates a new liquid chromatographic method for determining the phenoxyethanol used as an antimicrobial preservative in pharmaceutic and cosmetic products. The study applies the developed high-performance liquid chromatography HPLC method to pomade formulation using a diode array detector to determine the phenoxyethanol. Methods: The phenoxyethanol in the sample was analyzed in a C18 column 150 x 4.6 mm, 5 µm ID under chromatographic conditions where the flow rate was determined as 1.0 mLmin. The column oven was 30.0C, and phenoxyethanol was detected at 270 nm. Isocratic application of acetonitrile-water 50:50, vv was used as the mobile phase system. The validation of the developed method was performed according to the guidelines from the International Conference on Harmonisation ICH, 2005 Guidelines Q2 R1. Results: The linearity range of the phenoxyethanol was 0.125-0.375 mgmL, and the limits of detection and quantification were calculated as 31.25 ngmL and 125.0 ngmL, respectively. The assay recovery and precision of the phenoxyethanol from the pomade formulation were evaluated at 0.125 mgmL, 0.250 mgmL, and 0.375 mgmL concentrations. The mean recoveries for phenoxyethanol in the pomade formulation were calculated at 99.99-102.86. Conclusion: The validated method was successfully applied for determining phenoxyethanol in a topical formulation. The proposed method is cheap, fast, and simple and can be used safely for routine analysis.

 Background and Aims: This study develops and validates a new liquid chromatographic method for determining the phenoxyethanol used as an antimicrobial preservative in pharmaceutic and cosmetic products. The study applies the developed high-performance liquid chromatography HPLC method to pomade formulation using a diode array detector to determine the phenoxyethanol. Methods: The phenoxyethanol in the sample was analyzed in a C18 column 150 x 4.6 mm, 5 µm ID under chromatographic conditions where the flow rate was determined as 1.0 mLmin. The column oven was 30.0C, and phenoxyethanol was detected at 270 nm. Isocratic application of acetonitrile-water 50:50, vv was used as the mobile phase system. The validation of the developed method was performed according to the guidelines from the International Conference on Harmonisation ICH, 2005 Guidelines Q2 R1. Results: The linearity range of the phenoxyethanol was 0.125-0.375 mgmL, and the limits of detection and quantification were calculated as 31.25 ngmL and 125.0 ngmL, respectively. The assay recovery and precision of the phenoxyethanol from the pomade formulation were evaluated at 0.125 mgmL, 0.250 mgmL, and 0.375 mgmL concentrations. The mean recoveries for phenoxyethanol in the pomade formulation were calculated at 99.99-102.86. Conclusion: The validated method was successfully applied for determining phenoxyethanol in a topical formulation. The proposed method is cheap, fast, and simple and can be used safely for routine analysis.

 Background and Aims: This study develops and validates a new liquid chromatographic method for determining the phenoxyethanol used as an antimicrobial preservative in pharmaceutic and cosmetic products. The study applies the developed high-performance liquid chromatography HPLC method to pomade formulation using a diode array detector to determine the phenoxyethanol. Methods: The phenoxyethanol in the sample was analyzed in a C18 column 150 x 4.6 mm, 5 µm ID under chromatographic conditions where the flow rate was determined as 1.0 mLmin. The column oven was 30.0C, and phenoxyethanol was detected at 270 nm. Isocratic application of acetonitrile-water 50:50, vv was used as the mobile phase system. The validation of the developed method was performed according to the guidelines from the International Conference on Harmonisation ICH, 2005 Guidelines Q2 R1. Results: The linearity range of the phenoxyethanol was 0.125-0.375 mgmL, and the limits of detection and quantification were calculated as 31.25 ngmL and 125.0 ngmL, respectively. The assay recovery and precision of the phenoxyethanol from the pomade formulation were evaluated at 0.125 mgmL, 0.250 mgmL, and 0.375 mgmL concentrations. The mean recoveries for phenoxyethanol in the pomade formulation were calculated at 99.99-102.86. Conclusion: The validated method was successfully applied for determining phenoxyethanol in a topical formulation. The proposed method is cheap, fast, and simple and can be used safely for routine analysis.

Makale detayları

A new and rapid HPLC method for the determination of phenoxyethanol in topical formulation

Yazarlar:

Gamze Ergin Kızılçay | Sıdıka Toker | Dilek Matur

Yayıncı:

Istanbul University

Ana Başlıklar:

Pharmaceutical Analytical Chemistry

Dergi:

İstanbul Journal of Pharmacy Vol.54 Issue 1, pp.96-101

Belge Tipi:

Makale

Yayın Tarihi:

4/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001318876

pISSN:

2587-2087

Phenoxyethanol

HPLC

DAD

cream formulation

validation

antimicrobial preservative

Sayı

Makale detayları

A new and rapid HPLC method for the determination of phenoxyethanol in topical formulation

Yazarlar: Gamze Ergin Kızılçay | Sıdıka Toker | Dilek Matur

Yayıncı: Istanbul University

Ana Başlıklar: Pharmaceutical Analytical Chemistry

Dergi: İstanbul Journal of Pharmacy Vol.54 Issue 1, pp.96-101

Belge Tipi: Makale

Yayın Tarihi: 4/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001318876

pISSN: 2587-2087

Phenoxyethanol

HPLC

DAD

cream formulation

validation

antimicrobial preservative

Sayı

Yazarlar:

Gamze Ergin Kızılçay | Sıdıka Toker | Dilek Matur

Yayıncı:

Istanbul University

Ana Başlıklar:

Pharmaceutical Analytical Chemistry

Dergi:

İstanbul Journal of Pharmacy Vol.54 Issue 1, pp.96-101

Belge Tipi:

Makale

Yayın Tarihi:

4/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001318876

pISSN:

2587-2087