Production of a -Galactosidase using  Bacillus stearothermophilusvar. caldiloactis C953

Production Of A -galactosidase USİNG

Makale detayları

Production of a -Galactosidase using Bacillus stearothermophilusvar. caldiloactis C953

Yazarlar:

Production Of A -galactosidase USİNG

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 1999 Vol.23 Issue 1, pp.47-54

Belge Tipi:

Makale

Yayın Tarihi:

12/1998

Belge Dili:

Türkçe

Document ID (Org No.):

2000814325

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Anahtar kelimeler:

Özet:

 

An intracellular, thermostable a-galactosidase (a-D-galactoside galactohydrolase EC  3.2.1.22.) was produced from a strain of Bacillus stearothermophilus var. calidolactis C953. It was  found that the presence of NH 4 Cl in the fermentation medium affected microbial growth and that  optimum enzyme production occurred with peptone in various sources of nitrogen where NH 4 Cl was  used as the main inorganic nitrogen source. After peptone, in decreasing order of enzyme productivity,  came tryptone, soybean meal, soya-neutralized peptone and casamino acid. It was determined that, in  various carbon sources, raffinose produced the highest a-galactosidase activity, followed by melibiose,  D-galactose and molasses, and that in the medium with soybean meal extract the second highest level  of activity occurred. Soybean oligosaccharides (raffinose-like sugars), because of their present low  commercial value, could be of pratical use in the production of a-galactosidase.

 

An intracellular, thermostable a-galactosidase (a-D-galactoside galactohydrolase EC  3.2.1.22.) was produced from a strain of Bacillus stearothermophilus var. calidolactis C953. It was  found that the presence of NH 4 Cl in the fermentation medium affected microbial growth and that  optimum enzyme production occurred with peptone in various sources of nitrogen where NH 4 Cl was  used as the main inorganic nitrogen source. After peptone, in decreasing order of enzyme productivity,  came tryptone, soybean meal, soya-neutralized peptone and casamino acid. It was determined that, in  various carbon sources, raffinose produced the highest a-galactosidase activity, followed by melibiose,  D-galactose and molasses, and that in the medium with soybean meal extract the second highest level  of activity occurred. Soybean oligosaccharides (raffinose-like sugars), because of their present low  commercial value, could be of pratical use in the production of a-galactosidase.

Makale detayları

Production of a -Galactosidase using Bacillus stearothermophilusvar. caldiloactis C953

Yazarlar:

Production Of A -galactosidase USİNG

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 1999 Vol.23 Issue 1, pp.47-54

Belge Tipi:

Makale

Yayın Tarihi:

12/1998

Belge Dili:

Türkçe

Document ID (Org No.):

2000814325

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

a-Galactosidase

Microbial enzyme production

Bacillus stearothermophilus

Sayı

Makale detayları

Production of a -Galactosidase using Bacillus stearothermophilusvar. caldiloactis C953

Yazarlar: Production Of A -galactosidase USİNG

Yayıncı: TUBITAK

Dergi: Turkish Journal of Biology 1999 Vol.23 Issue 1, pp.47-54

Belge Tipi: Makale

Yayın Tarihi: 12/1998

Belge Dili: Türkçe

Document ID (Org No.): 2000814325

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

a-Galactosidase

Microbial enzyme production

Bacillus stearothermophilus

Sayı

Yazarlar:

Production Of A -galactosidase USİNG

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 1999 Vol.23 Issue 1, pp.47-54

Belge Tipi:

Makale

Yayın Tarihi:

12/1998

Belge Dili:

Türkçe

Document ID (Org No.):

2000814325

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152