Human embryonic stem cell N-glycan features relevant to pluripotency

Sabire KARAÇALI

Makale detayları

Human embryonic stem cell N-glycan features relevant to pluripotency

Yazarlar:

Sabire KARAÇALI

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2016 Vol.40 Issue 5, pp.1050-1058

Belge Tipi:

Makale

Yayın Tarihi:

9/2016

Belge Dili:

Türkçe

Document ID (Org No.):

2000811577

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Özet:

 

Human embryonic stem cells (hESCs) and reprogrammed human induced pluripotent stem cells (hiPSCs) proliferate indefinitely in the undifferentiated state (self-renewal) and differentiate into the three germ layers (and ultimately into all cell types) (pluripotency). Branching in the N-glycan core of hESCs/hiPSCs is limited. hESCs/hiPSCs have high mannose-type and biantennary complex-type core structures, indicating immature stages of N-glycoproteins. The two branches on the core consist of type 2 N-acetyllactosamine (LacNAc), completed mainly with α 2,6-linked sialic acid. Complex terminal fucosylation, particularly α 1,2-linked fucosylation, is another characteristic N-glycosylation feature of hESCs/hiPSCs. All of these structural features are probably associated with the pluripotency of hESCs/hiPSCs. In differentiated cells, two-, three-, and four-branched N-glycan core structures are seen and N-acetylhexosamine compositions increase in the branches. The elongation and size of the branches in the N-glycan cores increase by the addition of poly-LacNAc chains and are the typical features during differentiation. As α 1,2-linked terminal fucosylation disappears, α 1,6-linked core fucosylation increases. It is necessary to know the pluripotency-associated glycans in order to identify and isolate the pluripotent cells from heterogeneous populations containing differentiated cells.

Makale detayları

Human embryonic stem cell N-glycan features relevant to pluripotency

Yazarlar: Sabire KARAÇALI

Yayıncı: TUBITAK

Dergi: Turkish Journal of Biology 2016 Vol.40 Issue 5, pp.1050-1058

Belge Tipi: Makale

Yayın Tarihi: 9/2016

Belge Dili: Türkçe

Document ID (Org No.): 2000811577

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

Sayı

Yazarlar:

Sabire KARAÇALI

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2016 Vol.40 Issue 5, pp.1050-1058

Belge Tipi:

Makale

Yayın Tarihi:

9/2016

Belge Dili:

Türkçe

Document ID (Org No.):

2000811577

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152