Effects of Lead (II) Acetate and Salicylic Acid on Growth, Biochemical Parameters and miRNA156 Gene Expression in Medicago sativa L.

Mustafa Akçay

Makale detayları

Effects of Lead (II) Acetate and Salicylic Acid on Growth, Biochemical Parameters and miRNA156 Gene Expression in Medicago sativa L.

Yazarlar:

Mustafa Akçay

Yayıncı:

Mevlüt AKÇURA

Ana Başlıklar:

Plant Biochemistry | Plant Biotechnology | Plant Cell and Molecular Biology

Dergi:

Turkish Journal of Agricultural and Natural Sciences Vol.12 Issue 1, pp.74-86

Belge Tipi:

Makale

Yayın Tarihi:

1/2025

Belge Dili:

Türkçe

Document ID (Org No.):

2001339530

pISSN:

2148-3647

Anahtar kelimeler:

Özet:

 Plants can be exposed to unfavourable conditions in their environment that can occur in different ways that prevent them from growing, developing and photosynthesising. Lead II acetate is a toxic heavy metal that limits the growth and development of plants. Salicylic acid is a phytohormone that can be synthesised by plants and plays an important role in plant growth and development. In this study, Erzurum genotype of alfalfa Medicago sativa L., one of the most widely cultivated forage legume plants in Türkiye and used in animal nutrition, was used as material and the effects of lead II acetate PbCH3COO2, salicylic acid SA and combination treatments on various plant growth parameters, biochemical analyses and miRNA expression were investigated. Plants were grown for 28 days in a plant growth chamber adjusted to 25oC temperature, 168 photoperiod and 60 humidity and then the application phase was started. Concentrations of lead II acetate 100 and 1000 ppm, salicylic acid 0.5 mM and 2 mM and their combinations were applied to the soil of the plants as 40 ml solution for 8 days. At the end of this period, the plants were harvested and the effects of the treatments on root and plant lengths cm, root and leaf wet weights g, MDA content and miRNA 156 gene expression level were investigated. As a result of the study, lead II acetate 100 and 1000 ppm treatments decreased the root and plant lengths and leaf and root wet weights, increased the MDA content severely and down-regulated the miRNA156 gene expression level and negatively affected the growth and development of the plant, On the other hand, salicylic acid 0.5 mM and 2 mM treatments increased root and plant lengths, leaf and root wet weights, decreased MDA content, and up-regulated miRNA156 gene expression level and promoted plant growth and development.

 Plants can be exposed to unfavourable conditions in their environment that can occur in different ways that prevent them from growing, developing and photosynthesising. Lead II acetate is a toxic heavy metal that limits the growth and development of plants. Salicylic acid is a phytohormone that can be synthesised by plants and plays an important role in plant growth and development. In this study, Erzurum genotype of alfalfa Medicago sativa L., one of the most widely cultivated forage legume plants in Türkiye and used in animal nutrition, was used as material and the effects of lead II acetate PbCH3COO2, salicylic acid SA and combination treatments on various plant growth parameters, biochemical analyses and miRNA expression were investigated. Plants were grown for 28 days in a plant growth chamber adjusted to 25oC temperature, 168 photoperiod and 60 humidity and then the application phase was started. Concentrations of lead II acetate 100 and 1000 ppm, salicylic acid 0.5 mM and 2 mM and their combinations were applied to the soil of the plants as 40 ml solution for 8 days. At the end of this period, the plants were harvested and the effects of the treatments on root and plant lengths cm, root and leaf wet weights g, MDA content and miRNA 156 gene expression level were investigated. As a result of the study, lead II acetate 100 and 1000 ppm treatments decreased the root and plant lengths and leaf and root wet weights, increased the MDA content severely and down-regulated the miRNA156 gene expression level and negatively affected the growth and development of the plant, On the other hand, salicylic acid 0.5 mM and 2 mM treatments increased root and plant lengths, leaf and root wet weights, decreased MDA content, and up-regulated miRNA156 gene expression level and promoted plant growth and development.

 Plants can be exposed to unfavourable conditions in their environment that can occur in different ways that prevent them from growing, developing and photosynthesising. Lead II acetate is a toxic heavy metal that limits the growth and development of plants. Salicylic acid is a phytohormone that can be synthesised by plants and plays an important role in plant growth and development. In this study, Erzurum genotype of alfalfa Medicago sativa L., one of the most widely cultivated forage legume plants in Türkiye and used in animal nutrition, was used as material and the effects of lead II acetate PbCH3COO2, salicylic acid SA and combination treatments on various plant growth parameters, biochemical analyses and miRNA expression were investigated. Plants were grown for 28 days in a plant growth chamber adjusted to 25oC temperature, 168 photoperiod and 60 humidity and then the application phase was started. Concentrations of lead II acetate 100 and 1000 ppm, salicylic acid 0.5 mM and 2 mM and their combinations were applied to the soil of the plants as 40 ml solution for 8 days. At the end of this period, the plants were harvested and the effects of the treatments on root and plant lengths cm, root and leaf wet weights g, MDA content and miRNA 156 gene expression level were investigated. As a result of the study, lead II acetate 100 and 1000 ppm treatments decreased the root and plant lengths and leaf and root wet weights, increased the MDA content severely and down-regulated the miRNA156 gene expression level and negatively affected the growth and development of the plant, On the other hand, salicylic acid 0.5 mM and 2 mM treatments increased root and plant lengths, leaf and root wet weights, decreased MDA content, and up-regulated miRNA156 gene expression level and promoted plant growth and development.

 Bitkiler bulundukları çevre içinde büyüme, gelişim ve fotosentezini engelleyen farklı şekillerde meydana gelebilen olumsuz koşullara maruz kalabilmektedirler. Kurşun II asetat, bitkilerin büyüme ve gelişmesini sınırlayan toksik bir ağır metaldir. Salisilik asit, bitkiler tarafından sentezlenebilen ve bitkilerin büyüme ve gelişiminde önemli rol oynayan bir fitohormondur. Bu çalışmada, materyal olarak Türkiyede tarımı en fazla yapılan ve hayvan beslenmesinde kullanılan yem bitki türü baklagillerinden yonca Medicago sativa L. bitkisinin Erzurum genotipi kullanılmış ve kurşun II asetat PbCH3COO2, salisilik asit SA ve kombinasyon uygulamalarının bitki üzerinde çeşitli bitki büyüme parametreleri, biyokimyasal analiz ve miRNA ifadesi üzerine etkileri araştırılmıştır. Bitkiler, 25oC sıcaklık 168 fotoperiyot ve 60 neme ayarlanmış bitki büyütme kabini içerisinde 28 gün boyunca büyütülmüş ve ardından uygulama aşamasına geçilmiştir. Bitkilere uygulanan konsantrasyon uygulamaları kurşun II asetat 100 ve 1000 ppm, salisilik asit 0.5 mM ve 2 mM ve kombinasyonları şeklinde olup, konsantrasyonlar bitkilerin toprağına 8 gün boyunca 40 ml çözelti şeklinde uygulanmıştır. Bu süre sonunda bitkiler hasat edilerek yapılan uygulamaların kök ve bitki uzunlukları cm ile kök ve yaprak yaş ağırlıkları g, MDA içeriği ve miRNA156 gen anlatım seviyesi üzerine olan etkileri araştırılmıştır. Çalışma sonucunda, kurşun II asetat 100 ve 1000 ppm, uygulamalarının bitkinin kök ve bitki uzunlukları ile yaprak ve kök yaş ağırlıklarını azalttığı, MDA içeriğini şiddetli artırdığı ve miRNA156 gen anlatım seviyesini aşağı regüle ederek bitkinin büyüme ve gelişmesini olumsuz etkilediği, buna karşın salisilik asit 0,5 mM ve 2 mM uygulamaları bitkinin kök ve bitki uzunlukları ile yaprak ve kök yaş ağırlıklarını artırdığı, MDA içeriğini azalttığı ve miRNA156 gen anlatım seviyesini yukarı regüle ederek bitkinin büyüme ve gelişmesini sağladığı tespit edilmiştir.

Makale detayları

Effects of Lead (II) Acetate and Salicylic Acid on Growth, Biochemical Parameters and miRNA156 Gene Expression in Medicago sativa L.

Yazarlar:

Mustafa Akçay

Yayıncı:

Mevlüt AKÇURA

Ana Başlıklar:

Plant Biochemistry | Plant Biotechnology | Plant Cell and Molecular Biology

Dergi:

Turkish Journal of Agricultural and Natural Sciences Vol.12 Issue 1, pp.74-86

Belge Tipi:

Makale

Yayın Tarihi:

1/2025

Belge Dili:

Türkçe

Document ID (Org No.):

2001339530

pISSN:

2148-3647

Medicago sativa L.

Lead (II) acetate

Salicylic acid

Lipid peroxidation

miRNA156Medicago sativa L.

Kurşun (II) asetat

Salisilik asit

Lipit peroksidasyonu

miRNA156

Sayı

Makale detayları

Yazarlar: Mustafa Akçay

Yayıncı: Mevlüt AKÇURA

Ana Başlıklar: Plant Biochemistry | Plant Biotechnology | Plant Cell and Molecular Biology

Dergi: Turkish Journal of Agricultural and Natural Sciences Vol.12 Issue 1, pp.74-86

Belge Tipi: Makale

Yayın Tarihi: 1/2025

Belge Dili: Türkçe

Document ID (Org No.): 2001339530

pISSN: 2148-3647

Sayı

Yazarlar:

Mustafa Akçay

Yayıncı:

Mevlüt AKÇURA

Ana Başlıklar:

Plant Biochemistry | Plant Biotechnology | Plant Cell and Molecular Biology

Dergi:

Turkish Journal of Agricultural and Natural Sciences Vol.12 Issue 1, pp.74-86

Belge Tipi:

Makale

Yayın Tarihi:

1/2025

Belge Dili:

Türkçe

Document ID (Org No.):

2001339530

pISSN:

2148-3647