Influence of Enterococcus faecium, a probiotic component, on ion  channels in colon cancer

Fatma Seçer Çelik; Safaa Altveş

Makale detayları

Influence of Enterococcus faecium, a probiotic component, on ion channels in colon cancer

Yazarlar:

Fatma Seçer Çelik | Safaa Altveş

Yayıncı:

MediHealth Academy Yayıncılık

Ana Başlıklar:

Kanser Hücre Biyolojisi | Tıbbi Mikrobiyoloji (Diğer)

Dergi:

Anatolian Current Medical Journal Vol.7 Issue 1, pp.43-47

Belge Tipi:

Makale

Yayın Tarihi:

10/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001338829

pISSN:

2718-0115

Anahtar kelimeler:

Özet:

 Aims: This study aimed to investigate the effect of probiotic bacterium Enterococcus faecium E. faecium on the gene expression of ion channels in colon cancer cells. Methods: The cytotoxic effect on SW480 colon cancer cell line was analyzed by MTT cell proliferation test using E. faecium bacterial cell culture free supernatants. The effect of E. faecium on ion channel genes in SW480 cells was determined by qRT PCR analysis. STRING analysis was used to reveal protein-protein interactions of ion channel proteins. Bioinformatic analysis of healthy and colon cancer patient data on ion channels was revealed with GEPIA platform. Results: The SW480 cell lines viability was enhanced by E. fecieum bacterial cell culture free supernatants dosages of 0.5 and 1.09 mgml, but it dramatically declined between 2.187 and 17.5 mgml doses, according the MTT study. Analysis of gene expression revealed that the TRPV2 and TRPM8 genes had significantly increased. The examined ion channel proteins were discovered to be substantially linked to one another based on STRING analysis. The TRPM2 gene we looked at revealed a notable rise in colon cancer patients based on data from both healthy and colon cancer patients on the GEPIA platform. Conclusion: E. faecium has been shown to have many beneficial effects on health. Our study has shown that it also has an effect on ion channels in cancer cells and that ion channels are of great importance for cell survival and death.

 Aims: This study aimed to investigate the effect of probiotic bacterium Enterococcus faecium E. faecium on the gene expression of ion channels in colon cancer cells. Methods: The cytotoxic effect on SW480 colon cancer cell line was analyzed by MTT cell proliferation test using E. faecium bacterial cell culture free supernatants. The effect of E. faecium on ion channel genes in SW480 cells was determined by qRT PCR analysis. STRING analysis was used to reveal protein-protein interactions of ion channel proteins. Bioinformatic analysis of healthy and colon cancer patient data on ion channels was revealed with GEPIA platform. Results: The SW480 cell lines viability was enhanced by E. fecieum bacterial cell culture free supernatants dosages of 0.5 and 1.09 mgml, but it dramatically declined between 2.187 and 17.5 mgml doses, according the MTT study. Analysis of gene expression revealed that the TRPV2 and TRPM8 genes had significantly increased. The examined ion channel proteins were discovered to be substantially linked to one another based on STRING analysis. The TRPM2 gene we looked at revealed a notable rise in colon cancer patients based on data from both healthy and colon cancer patients on the GEPIA platform. Conclusion: E. faecium has been shown to have many beneficial effects on health. Our study has shown that it also has an effect on ion channels in cancer cells and that ion channels are of great importance for cell survival and death.

 Aims: This study aimed to investigate the effect of probiotic bacterium Enterococcus faecium E. faecium on the gene expression of ion channels in colon cancer cells. Methods: The cytotoxic effect on SW480 colon cancer cell line was analyzed by MTT cell proliferation test using E. faecium bacterial cell culture free supernatants. The effect of E. faecium on ion channel genes in SW480 cells was determined by qRT PCR analysis. STRING analysis was used to reveal protein-protein interactions of ion channel proteins. Bioinformatic analysis of healthy and colon cancer patient data on ion channels was revealed with GEPIA platform. Results: The SW480 cell lines viability was enhanced by E. fecieum bacterial cell culture free supernatants dosages of 0.5 and 1.09 mgml, but it dramatically declined between 2.187 and 17.5 mgml doses, according the MTT study. Analysis of gene expression revealed that the TRPV2 and TRPM8 genes had significantly increased. The examined ion channel proteins were discovered to be substantially linked to one another based on STRING analysis. The TRPM2 gene we looked at revealed a notable rise in colon cancer patients based on data from both healthy and colon cancer patients on the GEPIA platform. Conclusion: E. faecium has been shown to have many beneficial effects on health. Our study has shown that it also has an effect on ion channels in cancer cells and that ion channels are of great importance for cell survival and death.

 Aims: This study aimed to investigate the effect of probiotic bacterium Enterococcus faecium E. faecium on the gene expression of ion channels in colon cancer cells. Methods: The cytotoxic effect on SW480 colon cancer cell line was analyzed by MTT cell proliferation test using E. faecium bacterial cell culture free supernatants. The effect of E. faecium on ion channel genes in SW480 cells was determined by qRT PCR analysis. STRING analysis was used to reveal protein-protein interactions of ion channel proteins. Bioinformatic analysis of healthy and colon cancer patient data on ion channels was revealed with GEPIA platform. Results: The SW480 cell lines viability was enhanced by E. fecieum bacterial cell culture free supernatants dosages of 0.5 and 1.09 mgml, but it dramatically declined between 2.187 and 17.5 mgml doses, according the MTT study. Analysis of gene expression revealed that the TRPV2 and TRPM8 genes had significantly increased. The examined ion channel proteins were discovered to be substantially linked to one another based on STRING analysis. The TRPM2 gene we looked at revealed a notable rise in colon cancer patients based on data from both healthy and colon cancer patients on the GEPIA platform. Conclusion: E. faecium has been shown to have many beneficial effects on health. Our study has shown that it also has an effect on ion channels in cancer cells and that ion channels are of great importance for cell survival and death.

Makale detayları

Influence of Enterococcus faecium, a probiotic component, on ion channels in colon cancer

Yazarlar:

Fatma Seçer Çelik | Safaa Altveş

Yayıncı:

MediHealth Academy Yayıncılık

Ana Başlıklar:

Kanser Hücre Biyolojisi | Tıbbi Mikrobiyoloji (Diğer)

Dergi:

Anatolian Current Medical Journal Vol.7 Issue 1, pp.43-47

Belge Tipi:

Makale

Yayın Tarihi:

10/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001338829

pISSN:

2718-0115

Colon cancer

ion channels

microbiota

E. faecium

Sayı

Makale detayları

Influence of Enterococcus faecium, a probiotic component, on ion channels in colon cancer

Yazarlar: Fatma Seçer Çelik | Safaa Altveş

Yayıncı: MediHealth Academy Yayıncılık

Ana Başlıklar: Kanser Hücre Biyolojisi | Tıbbi Mikrobiyoloji (Diğer)

Dergi: Anatolian Current Medical Journal Vol.7 Issue 1, pp.43-47

Belge Tipi: Makale

Yayın Tarihi: 10/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001338829

pISSN: 2718-0115

Colon cancer

ion channels

microbiota

E. faecium

Sayı

Yazarlar:

Fatma Seçer Çelik | Safaa Altveş

Yayıncı:

MediHealth Academy Yayıncılık

Ana Başlıklar:

Kanser Hücre Biyolojisi | Tıbbi Mikrobiyoloji (Diğer)

Dergi:

Anatolian Current Medical Journal Vol.7 Issue 1, pp.43-47

Belge Tipi:

Makale

Yayın Tarihi:

10/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001338829

pISSN:

2718-0115