Formulation and validation of analytical methods for ursolic acid in Plantago major gel preparations

N.A.

Makale detayları

Formulation and validation of analytical methods for ursolic acid in Plantago major gel preparations

Yazarlar:

N.A.

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmacognosy

Dergi:

Journal of Research in Pharmacy Vol.27 Issue 1, pp.290-298

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001345959

pISSN:

2630-6344

Anahtar kelimeler:

Özet:

 Plantago major of the plantain family has been observed as a wound-healing plant species on account of its ursolic acid UA content. The studys objectives were twofold: to prepare UA gels from P. major and validate the analytical method using TLC-densitometer. Here, vacuum and open column chromatography were used to isolate UA from the methanol extract of P. major. Afterward, data on the compounds physical properties and infrared IR spectroscopy, electrospray ionization mass spectrometry ESI-MS, and Nuclear Magnetic Resonance 1H NMR, 13C NMR results were analyzed. Carbomer and hydroxy propyl methyl cellulose HPMC were used as gelling agents to make the UA gel. To validate the analytical method, TLC-densitometer was performed using TLC silica gel 60 F254 plates the stationary phase and a mixture of toluene: ethyl acetate: formic acid 8:2:0.1, the developing solvent. The results showed that the UA gel was successfully prepared using both gelling agents and that its physical properties i.e., visual forms, viscosity, and flow behavior and pH values were maintained throughout the 90-day accelerated stability test 402C; 755 RH. In addition, the method validation results indicated compliance with relevant parameters: linearity r0.998, limit of detection 4.55 ngspot and limit of quantification 15.17 ngspot, recovery 95.04-116.82, intraday precision RSD1.241.96 and interday precision RSD6.29. Overall, the UA gel is stable for 90 days accelerated stability test, and the TLC-densitometry proposed in the research can be used to identify and measure the chemical content of a gel preparation.

 Plantago major of the plantain family has been observed as a wound-healing plant species on account of its ursolic acid UA content. The studys objectives were twofold: to prepare UA gels from P. major and validate the analytical method using TLC-densitometer. Here, vacuum and open column chromatography were used to isolate UA from the methanol extract of P. major. Afterward, data on the compounds physical properties and infrared IR spectroscopy, electrospray ionization mass spectrometry ESI-MS, and Nuclear Magnetic Resonance 1H NMR, 13C NMR results were analyzed. Carbomer and hydroxy propyl methyl cellulose HPMC were used as gelling agents to make the UA gel. To validate the analytical method, TLC-densitometer was performed using TLC silica gel 60 F254 plates the stationary phase and a mixture of toluene: ethyl acetate: formic acid 8:2:0.1, the developing solvent. The results showed that the UA gel was successfully prepared using both gelling agents and that its physical properties i.e., visual forms, viscosity, and flow behavior and pH values were maintained throughout the 90-day accelerated stability test 402C; 755 RH. In addition, the method validation results indicated compliance with relevant parameters: linearity r0.998, limit of detection 4.55 ngspot and limit of quantification 15.17 ngspot, recovery 95.04-116.82, intraday precision RSD1.241.96 and interday precision RSD6.29. Overall, the UA gel is stable for 90 days accelerated stability test, and the TLC-densitometry proposed in the research can be used to identify and measure the chemical content of a gel preparation.

 Plantago major of the plantain family has been observed as a wound-healing plant species on account of its ursolic acid UA content. The studys objectives were twofold: to prepare UA gels from P. major and validate the analytical method using TLC-densitometer. Here, vacuum and open column chromatography were used to isolate UA from the methanol extract of P. major. Afterward, data on the compounds physical properties and infrared IR spectroscopy, electrospray ionization mass spectrometry ESI-MS, and Nuclear Magnetic Resonance 1H NMR, 13C NMR results were analyzed. Carbomer and hydroxy propyl methyl cellulose HPMC were used as gelling agents to make the UA gel. To validate the analytical method, TLC-densitometer was performed using TLC silica gel 60 F254 plates the stationary phase and a mixture of toluene: ethyl acetate: formic acid 8:2:0.1, the developing solvent. The results showed that the UA gel was successfully prepared using both gelling agents and that its physical properties i.e., visual forms, viscosity, and flow behavior and pH values were maintained throughout the 90-day accelerated stability test 402C; 755 RH. In addition, the method validation results indicated compliance with relevant parameters: linearity r0.998, limit of detection 4.55 ngspot and limit of quantification 15.17 ngspot, recovery 95.04-116.82, intraday precision RSD1.241.96 and interday precision RSD6.29. Overall, the UA gel is stable for 90 days accelerated stability test, and the TLC-densitometry proposed in the research can be used to identify and measure the chemical content of a gel preparation.

 Plantago major of the plantain family has been observed as a wound-healing plant species on account of its ursolic acid UA content. The studys objectives were twofold: to prepare UA gels from P. major and validate the analytical method using TLC-densitometer. Here, vacuum and open column chromatography were used to isolate UA from the methanol extract of P. major. Afterward, data on the compounds physical properties and infrared IR spectroscopy, electrospray ionization mass spectrometry ESI-MS, and Nuclear Magnetic Resonance 1H NMR, 13C NMR results were analyzed. Carbomer and hydroxy propyl methyl cellulose HPMC were used as gelling agents to make the UA gel. To validate the analytical method, TLC-densitometer was performed using TLC silica gel 60 F254 plates the stationary phase and a mixture of toluene: ethyl acetate: formic acid 8:2:0.1, the developing solvent. The results showed that the UA gel was successfully prepared using both gelling agents and that its physical properties i.e., visual forms, viscosity, and flow behavior and pH values were maintained throughout the 90-day accelerated stability test 402C; 755 RH. In addition, the method validation results indicated compliance with relevant parameters: linearity r0.998, limit of detection 4.55 ngspot and limit of quantification 15.17 ngspot, recovery 95.04-116.82, intraday precision RSD1.241.96 and interday precision RSD6.29. Overall, the UA gel is stable for 90 days accelerated stability test, and the TLC-densitometry proposed in the research can be used to identify and measure the chemical content of a gel preparation.

Makale detayları

Formulation and validation of analytical methods for ursolic acid in Plantago major gel preparations

Yazarlar:

N.A.

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmacognosy

Dergi:

Journal of Research in Pharmacy Vol.27 Issue 1, pp.290-298

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001345959

pISSN:

2630-6344

Gel

method validation

Plantago major

plantain

stability test

ursolic acid

Sayı

Makale detayları

Formulation and validation of analytical methods for ursolic acid in Plantago major gel preparations

Yazarlar: N.A.

Yayıncı: Marmara University

Ana Başlıklar: Pharmacognosy

Dergi: Journal of Research in Pharmacy Vol.27 Issue 1, pp.290-298

Belge Tipi: Makale

Yayın Tarihi: 6/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001345959

pISSN: 2630-6344

Gel

method validation

Plantago major

plantain

stability test

ursolic acid

Sayı

Yazarlar:

N.A.

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmacognosy

Dergi:

Journal of Research in Pharmacy Vol.27 Issue 1, pp.290-298

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001345959

pISSN:

2630-6344