Polyphenol Screening by LC-MS/MS and Antioxidant Properties of Telephium imperati

Abdussamat Güzel

Makale detayları

Polyphenol Screening by LC-MS/MS and Antioxidant Properties of Telephium imperati

Yazarlar:

Abdussamat Güzel

Yayıncı:

Inonu University

Ana Başlıklar:

Geleneksel | Tamamlayıcı ve Bütünleştirici Tıp (Diğer)

Dergi:

İnönü Üniversitesi Sağlık Hizmetleri Meslek Yüksek Okulu Dergisi Vol.12 Issue 1, pp.305-316

Belge Tipi:

Makale

Yayın Tarihi:

2/2024

Belge Dili:

Türkçe

Document ID (Org No.):

2001310179

pISSN:

2147-7892

Anahtar kelimeler:

Özet:

 Telephium imperati L., belonging to the Caryophyllaceae family, is among the plants used in traditional Anatolian folk medicine. In this study, the profile of phenolic compounds and antioxidant activities of T. imperati were investigated for the first time. Liquid chromatography-tandem mass spectrometry LC-MSMS system was applied to determine phenolic compositions. Spectrophotometry was used to evaluate potential antioxidant activities. The antioxidant capacity of the methanol extract of T. imperati was determined by applying ABTS 2,2-azino-di-3-ethylbenzothiazoline-6-sulfonic acid, CUPRAC reducing power of Cupric Cu2 ions, DPPH1,1-diphenyl-2-picryl-hydrazyl, and FRAP Ferric ions Fe3 reducing power tests. Compared to BHA, BHT and Trolox used as standards, T. imperati showed moderate antioxidant activity. In the analysis of 25 phenolic compounds by LC-MSMS, acetohydroxamic acid, catechin hydrate, syringic acid, caffeic acid, quercetin, luteolin, and kaempferol were determined.

 Telephium imperati L., belonging to the Caryophyllaceae family, is among the plants used in traditional Anatolian folk medicine. In this study, the profile of phenolic compounds and antioxidant activities of T. imperati were investigated for the first time. Liquid chromatography-tandem mass spectrometry LC-MSMS system was applied to determine phenolic compositions. Spectrophotometry was used to evaluate potential antioxidant activities. The antioxidant capacity of the methanol extract of T. imperati was determined by applying ABTS 2,2-azino-di-3-ethylbenzothiazoline-6-sulfonic acid, CUPRAC reducing power of Cupric Cu2 ions, DPPH1,1-diphenyl-2-picryl-hydrazyl, and FRAP Ferric ions Fe3 reducing power tests. Compared to BHA, BHT and Trolox used as standards, T. imperati showed moderate antioxidant activity. In the analysis of 25 phenolic compounds by LC-MSMS, acetohydroxamic acid, catechin hydrate, syringic acid, caffeic acid, quercetin, luteolin, and kaempferol were determined.

 Telephium imperati L., belonging to the Caryophyllaceae family, is among the plants used in traditional Anatolian folk medicine. In this study, the profile of phenolic compounds and antioxidant activities of T. imperati were investigated for the first time. Liquid chromatography-tandem mass spectrometry LC-MSMS system was applied to determine phenolic compositions. Spectrophotometry was used to evaluate potential antioxidant activities. The antioxidant capacity of the methanol extract of T. imperati was determined by applying ABTS 2,2-azino-di-3-ethylbenzothiazoline-6-sulfonic acid, CUPRAC reducing power of Cupric Cu2 ions, DPPH1,1-diphenyl-2-picryl-hydrazyl, and FRAP Ferric ions Fe3 reducing power tests. Compared to BHA, BHT and Trolox used as standards, T. imperati showed moderate antioxidant activity. In the analysis of 25 phenolic compounds by LC-MSMS, acetohydroxamic acid, catechin hydrate, syringic acid, caffeic acid, quercetin, luteolin, and kaempferol were determined.

 Caryophyllaceae familyasına ait olan Telephium imperati L. geleneksel Anadolu halk hekimliğinde kullanılan bitkiler arasında yer alır. Bu çalışmada T. imperatinin fenolik bileşiklerin profili ve antioksidan aktiviteleri ilk defa araştırıldı. Fenolik bileşimlerini belirlemek için sıvı kromatografi-tandem kütle spektrometresi LC-MSMS sistemi uygulandı. Potansiyel antioksidan aktivitelerini değerlendirmek için spektrofotometre kullanıldı. ABTS 2,2-azino-di-3-etilbenzotiyazolin-6-sülfonik asit, CUPRAC Kuprik Cu2 iyonlarını indirgeme gücü, DPPH1,1-difenil-2-pikril-hidrazil ve FRAP Ferrik iyonları Fe3 indirgeme gücü testleri uygulanarak T. imperatinin metanol ekstraktının antioksidan kapasitesi belirlendi. Standart olarak kullanılan BHA, BHT ve Troloks göre T. imperati orta düzeyde antioksidan aktivite göstermiştir. LC-MSMS ile 25 fenolik bileşik analizinde asetohidroksamik asit, kateşin hidrat, siringik asit, kafeik asit, kuersetin, luteolin, kaempferol belirlenmiştir.

Makale detayları

Polyphenol Screening by LC-MS/MS and Antioxidant Properties of Telephium imperati

Yazarlar:

Abdussamat Güzel

Yayıncı:

Inonu University

Ana Başlıklar:

Geleneksel | Tamamlayıcı ve Bütünleştirici Tıp (Diğer)

Dergi:

İnönü Üniversitesi Sağlık Hizmetleri Meslek Yüksek Okulu Dergisi Vol.12 Issue 1, pp.305-316

Belge Tipi:

Makale

Yayın Tarihi:

2/2024

Belge Dili:

Türkçe

Document ID (Org No.):

2001310179

pISSN:

2147-7892

Antioxidant

LC-MS/MS

Polyphenol

Telephium imperatiAntioksidan

LC-MS/MS

Polifenol

Telephium imperati

Sayı

Makale detayları

Yazarlar: Abdussamat Güzel

Yayıncı: Inonu University

Ana Başlıklar: Geleneksel | Tamamlayıcı ve Bütünleştirici Tıp (Diğer)

Dergi: İnönü Üniversitesi Sağlık Hizmetleri Meslek Yüksek Okulu Dergisi Vol.12 Issue 1, pp.305-316

Belge Tipi: Makale

Yayın Tarihi: 2/2024

Belge Dili: Türkçe

Document ID (Org No.): 2001310179

pISSN: 2147-7892

Sayı

Yazarlar:

Abdussamat Güzel

Yayıncı:

Inonu University

Ana Başlıklar:

Geleneksel | Tamamlayıcı ve Bütünleştirici Tıp (Diğer)

Dergi:

İnönü Üniversitesi Sağlık Hizmetleri Meslek Yüksek Okulu Dergisi Vol.12 Issue 1, pp.305-316

Belge Tipi:

Makale

Yayın Tarihi:

2/2024

Belge Dili:

Türkçe

Document ID (Org No.):

2001310179

pISSN:

2147-7892