Chronology of estrogen receptor expression in testes of mouse embryos

INSAF JASIM MAHMOUD; MOHAMMAD ODA SELMAN; WAMEEDH RAAD ABDULMALIK SHEBEB

Makale detayları

Chronology of estrogen receptor expression in testes of mouse embryos

Yazarlar:

INSAF JASIM MAHMOUD | MOHAMMAD ODA SELMAN | WAMEEDH RAAD ABDULMALIK SHEBEB

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2015 Vol.45 Issue 3, pp.526-533

Belge Tipi:

Makale

Yayın Tarihi:

8/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000752652

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

Anahtar kelimeler:

Özet:

 

Background/aim: To localize and determine the time of expression of estrogen receptors (ERs) in testes of mouse embryos by histology and immunohistochemistry.   Materials and methods: Fifty-four mature Swiss-Webster mice (Mus musculus) were used. Group 1 consisted of 34 mature pregnant females, 3 of which were sacrificed every day from 10.5 to 20.5 days postcoitus (dpc). One testis was removed from their embryos and processed for histology and immunohistochemistry. Group 2 consisted of 20 postnatal mice: 5 postpartum (P0) males, 5 males 4 weeks old, 5 males 8 weeks old, and 5 females 4 weeks old.   Results: The first nuclear detection of ERs was observed in embryonic male gonads at 11.5 dpc, and a highly significant increase (P < 0.01) was observed at embryonic days 11.5, 13.5, 15.5, 16.5, 17.5, and 19.5, with a peak at 17.5 dpc and continuing to 20.5 dpc. ER expression increased further after birth.  Conclusion: Expression of ERs occurred at certain days during mouse embryonic development, indicating the need for estrogen for certain metabolic or morphological events occurring at these days. After birth, estrogen played an important role in proliferation and maturation of certain cells in the testes. Another rise in ER expression occurred during puberty in the mature testis.

 

Background/aim: To localize and determine the time of expression of estrogen receptors (ERs) in testes of mouse embryos by histology and immunohistochemistry.   Materials and methods: Fifty-four mature Swiss-Webster mice (Mus musculus) were used. Group 1 consisted of 34 mature pregnant females, 3 of which were sacrificed every day from 10.5 to 20.5 days postcoitus (dpc). One testis was removed from their embryos and processed for histology and immunohistochemistry. Group 2 consisted of 20 postnatal mice: 5 postpartum (P0) males, 5 males 4 weeks old, 5 males 8 weeks old, and 5 females 4 weeks old.   Results: The first nuclear detection of ERs was observed in embryonic male gonads at 11.5 dpc, and a highly significant increase (P < 0.01) was observed at embryonic days 11.5, 13.5, 15.5, 16.5, 17.5, and 19.5, with a peak at 17.5 dpc and continuing to 20.5 dpc. ER expression increased further after birth.  Conclusion: Expression of ERs occurred at certain days during mouse embryonic development, indicating the need for estrogen for certain metabolic or morphological events occurring at these days. After birth, estrogen played an important role in proliferation and maturation of certain cells in the testes. Another rise in ER expression occurred during puberty in the mature testis.

Makale detayları

Chronology of estrogen receptor expression in testes of mouse embryos

Yazarlar:

INSAF JASIM MAHMOUD | MOHAMMAD ODA SELMAN | WAMEEDH RAAD ABDULMALIK SHEBEB

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2015 Vol.45 Issue 3, pp.526-533

Belge Tipi:

Makale

Yayın Tarihi:

8/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000752652

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

Expression of estrogen receptors

testis of mouse embryo

Sayı

Makale detayları

Chronology of estrogen receptor expression in testes of mouse embryos

Yazarlar: INSAF JASIM MAHMOUD | MOHAMMAD ODA SELMAN | WAMEEDH RAAD ABDULMALIK SHEBEB

Yayıncı: TUBITAK

Dergi: Turkish Journal of Medical Sciences 2015 Vol.45 Issue 3, pp.526-533

Belge Tipi: Makale

Yayın Tarihi: 8/2015

Belge Dili: Türkçe

Document ID (Org No.): 2000752652

Sıklık: İki Aylık

eISSN: 1303-6165

pISSN: 1300-0144

Expression of estrogen receptors

testis of mouse embryo

Sayı

Yazarlar:

INSAF JASIM MAHMOUD | MOHAMMAD ODA SELMAN | WAMEEDH RAAD ABDULMALIK SHEBEB

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2015 Vol.45 Issue 3, pp.526-533

Belge Tipi:

Makale

Yayın Tarihi:

8/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000752652

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144