Biofilm formation and antibiotic resistance in patients with urolithiasis: assessment of phenotypic and genotypic

Mohammed Munadi Mohammed Mohammed; Serhat Sirekbasan; Asal Aziz Tawfeeq

Makale detayları

Biofilm formation and antibiotic resistance in patients with urolithiasis: assessment of phenotypic and genotypic

Yazarlar:

Mohammed Munadi Mohammed Mohammed | Serhat Sirekbasan | Asal Aziz Tawfeeq

Yayıncı:

Adıyaman University

Ana Başlıklar:

Tıbbi Bakteriyoloji

Dergi:

Adıyaman Üniversitesi Sağlık Bilimleri Dergisi Vol.10 Issue 1, pp.16-23

Belge Tipi:

Makale

Yayın Tarihi:

4/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001315235

pISSN:

2458-9179

Anahtar kelimeler:

Özet:

 Aim: Urolithiasis is a common public health problem that significantly impacts the community. The objective of this study was to identify the prevalent pathogens responsible for urinary tract infections in patients with urolithiasis and investigate the biofilm forming ability of these strains phenotypically and molecularly. Materials and Methods: A total of 100 patients who presented to Kirkuk Training and Research Hospital with symptoms of urinary tract infection and were diagnosed with kidney stones were included in our study conducted between May 2021 and November 2022. Clinically significant bacteria from urine samples were identified using routine conventional methods. Biofilm formation of the identified strains was examined by microplate method. Results: The most frequently isolated agents were Escherichia coli n:36 and Proteus mirabilis n:17. Biofilm formation was detected in 89 of E. coli strains and 94 of P. mirabilis strains. Conclusion: The results obtained are important in terms of high biofilm formation, especially in E. coli and P. mirabilis strains, and the frequent presence of genes related to this biofilm formation.

 Aim: Urolithiasis is a common public health problem that significantly impacts the community. The objective of this study was to identify the prevalent pathogens responsible for urinary tract infections in patients with urolithiasis and investigate the biofilm forming ability of these strains phenotypically and molecularly. Materials and Methods: A total of 100 patients who presented to Kirkuk Training and Research Hospital with symptoms of urinary tract infection and were diagnosed with kidney stones were included in our study conducted between May 2021 and November 2022. Clinically significant bacteria from urine samples were identified using routine conventional methods. Biofilm formation of the identified strains was examined by microplate method. Results: The most frequently isolated agents were Escherichia coli n:36 and Proteus mirabilis n:17. Biofilm formation was detected in 89 of E. coli strains and 94 of P. mirabilis strains. Conclusion: The results obtained are important in terms of high biofilm formation, especially in E. coli and P. mirabilis strains, and the frequent presence of genes related to this biofilm formation.

 Aim: Urolithiasis is a common public health problem that significantly impacts the community. The objective of this study was to identify the prevalent pathogens responsible for urinary tract infections in patients with urolithiasis and investigate the biofilm forming ability of these strains phenotypically and molecularly. Materials and Methods: A total of 100 patients who presented to Kirkuk Training and Research Hospital with symptoms of urinary tract infection and were diagnosed with kidney stones were included in our study conducted between May 2021 and November 2022. Clinically significant bacteria from urine samples were identified using routine conventional methods. Biofilm formation of the identified strains was examined by microplate method. Results: The most frequently isolated agents were Escherichia coli n:36 and Proteus mirabilis n:17. Biofilm formation was detected in 89 of E. coli strains and 94 of P. mirabilis strains. Conclusion: The results obtained are important in terms of high biofilm formation, especially in E. coli and P. mirabilis strains, and the frequent presence of genes related to this biofilm formation.

 Amaç: Ürolitiyazis, toplumu önemli ölçüde etkileyen yaygın bir halk sağlığı sorunudur. Bu çalışmanın amacı; ürolitiyazisli hastalarda gelişen üriner sistem enfeksiyonlarının yaygın etkenlerini saptamak ve bu kökenlerin biyofilm oluşturma yeteneğinin fenotipik ve moleküler olarak araştırılmasıdır. Gereç ve Yöntem: Mayıs 2021 ile Kasım 2022 tarihleri arasında yürüttüğümüz çalışmamıza Kerkük Eğitim ve Araştırma Hastanesine idrar yolu enfeksiyonu semptomları ile başvuran ve böbrek taşı tanısı alan toplam 100 hasta dahil edildi. Alınan idrar örneklerinden klinik olarak önemli bakterilerin tanımlanması için rutin konvansiyonel yöntemler kullanıldı. Saptanan kökenlerin biyofilm oluşumu mikroplak yöntemi ile incelendi. Bulgular: En sık izole edilen etkenler Escherichia coli n:36 ve Proteus mirabilis n:17 olarak saptandı. E. coli kökenlerinin 89unda, P. mirabilis kökenlerinin ise 94ünde biyofilm oluşumu gözlendi. Sonuç: Elde edilen sonuçlar, özellikle E. coli ve P. mirabilis kökenlerinde yüksek biyofilm oluşumu ve bu biyofilm oluşumuna ilişkin genlerin sıklıkla bulunması açısından önemlidir.

Makale detayları

Biofilm formation and antibiotic resistance in patients with urolithiasis: assessment of phenotypic and genotypic

Yazarlar:

Mohammed Munadi Mohammed Mohammed | Serhat Sirekbasan | Asal Aziz Tawfeeq

Yayıncı:

Adıyaman University

Ana Başlıklar:

Tıbbi Bakteriyoloji

Dergi:

Adıyaman Üniversitesi Sağlık Bilimleri Dergisi Vol.10 Issue 1, pp.16-23

Belge Tipi:

Makale

Yayın Tarihi:

4/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001315235

pISSN:

2458-9179

Urolithiasis

Biofilm

Kidney stones

Resistance genesÜrolitiyazis

Biyofilm

Böbrek taşı

Direnç genleri

Sayı

Makale detayları

Yazarlar: Mohammed Munadi Mohammed Mohammed | Serhat Sirekbasan | Asal Aziz Tawfeeq

Yayıncı: Adıyaman University

Ana Başlıklar: Tıbbi Bakteriyoloji

Dergi: Adıyaman Üniversitesi Sağlık Bilimleri Dergisi Vol.10 Issue 1, pp.16-23

Belge Tipi: Makale

Yayın Tarihi: 4/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001315235

pISSN: 2458-9179

Sayı

Yazarlar:

Mohammed Munadi Mohammed Mohammed | Serhat Sirekbasan | Asal Aziz Tawfeeq

Yayıncı:

Adıyaman University

Ana Başlıklar:

Tıbbi Bakteriyoloji

Dergi:

Adıyaman Üniversitesi Sağlık Bilimleri Dergisi Vol.10 Issue 1, pp.16-23

Belge Tipi:

Makale

Yayın Tarihi:

4/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001315235

pISSN:

2458-9179