Measurement of Total Iron in Breast Tissue Samples by Inductively Coupled Plasma-Mass Spectrometry

Mete Bora Tüzüner; Burcin Tüzüner

Makale detayları

Measurement of Total Iron in Breast Tissue Samples by Inductively Coupled Plasma-Mass Spectrometry

Yazarlar:

Mete Bora Tüzüner | Burcin Tüzüner

Yayıncı:

Istanbul University

Ana Başlıklar:

Biochemistry and Cell Biology (Other)

Dergi:

European Journal of Biology Vol.82 Issue 2, pp.270-279

Belge Tipi:

Makale

Yayın Tarihi:

12/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001310878

pISSN:

2602-2575

Anahtar kelimeler:

Özet:

 Objective: Breast cancer is the commonest and the deadliest malignancy among women. Iron is known as an essential element for cell growth and division. The connection between oxidative stress, antioxidants, and progression, aggressiveness, and recurrence of breast cancer, is widely recognized to involve impaired iron metabolism. The purpose of this study was to investigate the connection between the tumor characteristics and the levels of total iron in breast tissues by employing state of the art technology, inductively-coupled plasmamass spectrometry ICP-MS with our in-house analysis methodology for tissue samples. Materials and Methods: Iron contents were determined in 25 tissue sets matched tumor and peritumoral tissues collected from 25 women diagnosed with invasive ductal breast cancer. In addition, three cancer-free breast tissues, obtained from breast reduction surgery, were analyzed as a normal group. Collected samples were digested and introduced to ICP-MS for iron analysis. Results: Our method showed a low rate of measurement error 10. A highly significant p0.001 3 fold difference of iron concentrations were observed in tumors 24.736.15 ppbmg as compared to peritumoral tissues 9.104.84 ppbmg. As the grade and stage of the cancer increases, iron levels in tumor tissues were also found to be increased p0.006, p0.022 respectively. Conclusion: Our ICP-MS based method can be reliably performed at the established conditions for tissue specimens, and also have potential to be used in clinical practice. Understanding the relationship between tissue iron levels and tumor characteristics is essential in identifying potential prevention and treatment strategies.

 Objective: Breast cancer is the commonest and the deadliest malignancy among women. Iron is known as an essential element for cell growth and division. The connection between oxidative stress, antioxidants, and progression, aggressiveness, and recurrence of breast cancer, is widely recognized to involve impaired iron metabolism. The purpose of this study was to investigate the connection between the tumor characteristics and the levels of total iron in breast tissues by employing state of the art technology, inductively-coupled plasmamass spectrometry ICP-MS with our in-house analysis methodology for tissue samples. Materials and Methods: Iron contents were determined in 25 tissue sets matched tumor and peritumoral tissues collected from 25 women diagnosed with invasive ductal breast cancer. In addition, three cancer-free breast tissues, obtained from breast reduction surgery, were analyzed as a normal group. Collected samples were digested and introduced to ICP-MS for iron analysis. Results: Our method showed a low rate of measurement error 10. A highly significant p0.001 3 fold difference of iron concentrations were observed in tumors 24.736.15 ppbmg as compared to peritumoral tissues 9.104.84 ppbmg. As the grade and stage of the cancer increases, iron levels in tumor tissues were also found to be increased p0.006, p0.022 respectively. Conclusion: Our ICP-MS based method can be reliably performed at the established conditions for tissue specimens, and also have potential to be used in clinical practice. Understanding the relationship between tissue iron levels and tumor characteristics is essential in identifying potential prevention and treatment strategies.

 Objective: Breast cancer is the commonest and the deadliest malignancy among women. Iron is known as an essential element for cell growth and division. The connection between oxidative stress, antioxidants, and progression, aggressiveness, and recurrence of breast cancer, is widely recognized to involve impaired iron metabolism. The purpose of this study was to investigate the connection between the tumor characteristics and the levels of total iron in breast tissues by employing state of the art technology, inductively-coupled plasmamass spectrometry ICP-MS with our in-house analysis methodology for tissue samples. Materials and Methods: Iron contents were determined in 25 tissue sets matched tumor and peritumoral tissues collected from 25 women diagnosed with invasive ductal breast cancer. In addition, three cancer-free breast tissues, obtained from breast reduction surgery, were analyzed as a normal group. Collected samples were digested and introduced to ICP-MS for iron analysis. Results: Our method showed a low rate of measurement error 10. A highly significant p0.001 3 fold difference of iron concentrations were observed in tumors 24.736.15 ppbmg as compared to peritumoral tissues 9.104.84 ppbmg. As the grade and stage of the cancer increases, iron levels in tumor tissues were also found to be increased p0.006, p0.022 respectively. Conclusion: Our ICP-MS based method can be reliably performed at the established conditions for tissue specimens, and also have potential to be used in clinical practice. Understanding the relationship between tissue iron levels and tumor characteristics is essential in identifying potential prevention and treatment strategies.

 Objective: Breast cancer is the commonest and the deadliest malignancy among women. Iron is known as an essential element for cell growth and division. The connection between oxidative stress, antioxidants, and progression, aggressiveness, and recurrence of breast cancer, is widely recognized to involve impaired iron metabolism. The purpose of this study was to investigate the connection between the tumor characteristics and the levels of total iron in breast tissues by employing state of the art technology, inductively-coupled plasmamass spectrometry ICP-MS with our in-house analysis methodology for tissue samples. Materials and Methods: Iron contents were determined in 25 tissue sets matched tumor and peritumoral tissues collected from 25 women diagnosed with invasive ductal breast cancer. In addition, three cancer-free breast tissues, obtained from breast reduction surgery, were analyzed as a normal group. Collected samples were digested and introduced to ICP-MS for iron analysis. Results: Our method showed a low rate of measurement error 10. A highly significant p0.001 3 fold difference of iron concentrations were observed in tumors 24.736.15 ppbmg as compared to peritumoral tissues 9.104.84 ppbmg. As the grade and stage of the cancer increases, iron levels in tumor tissues were also found to be increased p0.006, p0.022 respectively. Conclusion: Our ICP-MS based method can be reliably performed at the established conditions for tissue specimens, and also have potential to be used in clinical practice. Understanding the relationship between tissue iron levels and tumor characteristics is essential in identifying potential prevention and treatment strategies.

Makale detayları

Measurement of Total Iron in Breast Tissue Samples by Inductively Coupled Plasma-Mass Spectrometry

Yazarlar:

Mete Bora Tüzüner | Burcin Tüzüner

Yayıncı:

Istanbul University

Ana Başlıklar:

Biochemistry and Cell Biology (Other)

Dergi:

European Journal of Biology Vol.82 Issue 2, pp.270-279

Belge Tipi:

Makale

Yayın Tarihi:

12/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001310878

pISSN:

2602-2575

Iron

Breast cancer

ROS

ICP-MS

Sayı

Makale detayları

Measurement of Total Iron in Breast Tissue Samples by Inductively Coupled Plasma-Mass Spectrometry

Yazarlar: Mete Bora Tüzüner | Burcin Tüzüner

Yayıncı: Istanbul University

Ana Başlıklar: Biochemistry and Cell Biology (Other)

Dergi: European Journal of Biology Vol.82 Issue 2, pp.270-279

Belge Tipi: Makale

Yayın Tarihi: 12/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001310878

pISSN: 2602-2575

Iron

Breast cancer

ROS

ICP-MS

Sayı

Yazarlar:

Mete Bora Tüzüner | Burcin Tüzüner

Yayıncı:

Istanbul University

Ana Başlıklar:

Biochemistry and Cell Biology (Other)

Dergi:

European Journal of Biology Vol.82 Issue 2, pp.270-279

Belge Tipi:

Makale

Yayın Tarihi:

12/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001310878

pISSN:

2602-2575