ANTIOXIDANT, ENZYME INHIBITORY, AND CYTOTOXIC ACTIVITY SCREENING OF MYRTUS COMMUNIS L.

Esra Köngül Şafak; Selen İlgün; Kübra Nur Çoban; Sena Akçakaya Mutlu; Halil Yılmaz; Gökçe Şeker Karatoprak

Makale detayları

ANTIOXIDANT, ENZYME INHIBITORY, AND CYTOTOXIC ACTIVITY SCREENING OF MYRTUS COMMUNIS L.

Yazarlar:

Esra Köngül Şafak | Selen İlgün | Kübra Nur Çoban | Sena Akçakaya Mutlu | Halil Yılmaz | Gökçe Şeker Karatoprak

Yayıncı:

Ankara University

Ana Başlıklar:

Eczacılık ve İlaç Bilimleri

Dergi:

Ankara Üniversitesi Eczacılık Fakültesi Dergisi Vol.47 Issue 3, pp.827-835

Belge Tipi:

Makale

Yayın Tarihi:

9/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001291230

pISSN:

1015-3918

Anahtar kelimeler:

Özet:

 Objective: The present study aimed to evaluate the biological activities of Myrtus communis L., which has traditional medicinal use for different purposes, from a broad perspective. Material and Method: The antioxidant DPPH, ABTS, total phenol, and flavonoid amounts anti-inflammatory LOX enzyme inhibition, antidiabetic α-glucosidase enzyme inhibition, and cytotoxic properties MTT assay of a 70 methanol extract made from leaves and dichloromethane, butanol, ethyl acetate, and the residual water fractions were investigated. Result and Discussion: The DPPH radical scavenging effect of the ethyl acetate fraction, with the highest total phenol and flavonoid content, was found to be 1.4 µgml, and the ABTS radical scavenging effect was 2.58 mmolTrolox. The most potent inhibitor of LOX IC50: 31.17 µgml and α-glucosidase 96 inhibition at 0.5 mgml enzymes was determined as ethyl acetate extract. Dichloromethane fraction was shown to have the most cytotoxic activity in both Hela and Colo cell lines. This research has given us a better understanding of the traditional use of the M. communis plant, which stands out for its therapeutic properties.

 Objective: The present study aimed to evaluate the biological activities of Myrtus communis L., which has traditional medicinal use for different purposes, from a broad perspective. Material and Method: The antioxidant DPPH, ABTS, total phenol, and flavonoid amounts anti-inflammatory LOX enzyme inhibition, antidiabetic α-glucosidase enzyme inhibition, and cytotoxic properties MTT assay of a 70 methanol extract made from leaves and dichloromethane, butanol, ethyl acetate, and the residual water fractions were investigated. Result and Discussion: The DPPH radical scavenging effect of the ethyl acetate fraction, with the highest total phenol and flavonoid content, was found to be 1.4 µgml, and the ABTS radical scavenging effect was 2.58 mmolTrolox. The most potent inhibitor of LOX IC50: 31.17 µgml and α-glucosidase 96 inhibition at 0.5 mgml enzymes was determined as ethyl acetate extract. Dichloromethane fraction was shown to have the most cytotoxic activity in both Hela and Colo cell lines. This research has given us a better understanding of the traditional use of the M. communis plant, which stands out for its therapeutic properties.

 Objective: The present study aimed to evaluate the biological activities of Myrtus communis L., which has traditional medicinal use for different purposes, from a broad perspective. Material and Method: The antioxidant DPPH, ABTS, total phenol, and flavonoid amounts anti-inflammatory LOX enzyme inhibition, antidiabetic α-glucosidase enzyme inhibition, and cytotoxic properties MTT assay of a 70 methanol extract made from leaves and dichloromethane, butanol, ethyl acetate, and the residual water fractions were investigated. Result and Discussion: The DPPH radical scavenging effect of the ethyl acetate fraction, with the highest total phenol and flavonoid content, was found to be 1.4 µgml, and the ABTS radical scavenging effect was 2.58 mmolTrolox. The most potent inhibitor of LOX IC50: 31.17 µgml and α-glucosidase 96 inhibition at 0.5 mgml enzymes was determined as ethyl acetate extract. Dichloromethane fraction was shown to have the most cytotoxic activity in both Hela and Colo cell lines. This research has given us a better understanding of the traditional use of the M. communis plant, which stands out for its therapeutic properties.

 Amaç: Bu çalışmada farklı amaçlarla geleneksel tıbbi kullanıma sahip olan Myrtus communis L.nin biyolojik aktivitelerinin geniş bir perspektiften değerlendirilmesi amaçlanmıştır. Gereç ve Yöntem: Yapraklardan yapılan 70 metanol ekstresinin ve diklorometan, butanol, etil asetat ve kalan su fraksiyonlarının antioksidan DPPH, ABTS, toplam fenol ve flavonoit miktarları, antienflamatuvar LOX enzim inhibisyonu, antidiyabetik α-glukozidaz enzim inhibisyonu ve sitotoksik özellikleri MTT testi incelenmiştir. Sonuç ve Tartışma: En yüksek toplam fenol ve flavonoit içeriğine sahip etil asetat fraksiyonunun DPPH radikal süpürücü etkisi 1,4 µgml ve ABTS radikal süpürücü etkisi 2,58 mmollTrolox olarak bulunmuştur. LOX IC50: 31,17 µgml ve α-glukozidaz 0,5 mgmlde 96 inhibisyon enzimlerinin en güçlü inhibitörü etil asetat ekstresi olarak belirlenmiştir. Diklorometan fraksiyonunun hem Hela hem de Colo hücre hatlarında en fazla sitotoksik aktiviteye sahip olduğu gösterilmiştir. Bu araştırma, tedavi edici özellikleriyle öne çıkan M. communis bitkisinin geleneksel kullanımını daha iyi anlamamızı sağlamıştır.

Makale detayları

ANTIOXIDANT, ENZYME INHIBITORY, AND CYTOTOXIC ACTIVITY SCREENING OF MYRTUS COMMUNIS L.

Yazarlar:

Esra Köngül Şafak | Selen İlgün | Kübra Nur Çoban | Sena Akçakaya Mutlu | Halil Yılmaz | Gökçe Şeker Karatoprak

Yayıncı:

Ankara University

Ana Başlıklar:

Eczacılık ve İlaç Bilimleri

Dergi:

Ankara Üniversitesi Eczacılık Fakültesi Dergisi Vol.47 Issue 3, pp.827-835

Belge Tipi:

Makale

Yayın Tarihi:

9/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001291230

pISSN:

1015-3918

Antioxidant

cytotoxic activity

enzyme inhibition

Myrtus communisAntioksidan

sitotoksik aktivite

enzim inhibisyonu

Myrtus communis

Sayı

Makale detayları

Yazarlar: Esra Köngül Şafak | Selen İlgün | Kübra Nur Çoban | Sena Akçakaya Mutlu | Halil Yılmaz | Gökçe Şeker Karatoprak

Yayıncı: Ankara University

Ana Başlıklar: Eczacılık ve İlaç Bilimleri

Dergi: Ankara Üniversitesi Eczacılık Fakültesi Dergisi Vol.47 Issue 3, pp.827-835

Belge Tipi: Makale

Yayın Tarihi: 9/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001291230

pISSN: 1015-3918

Sayı

Yazarlar:

Esra Köngül Şafak | Selen İlgün | Kübra Nur Çoban | Sena Akçakaya Mutlu | Halil Yılmaz | Gökçe Şeker Karatoprak

Yayıncı:

Ankara University

Ana Başlıklar:

Eczacılık ve İlaç Bilimleri

Dergi:

Ankara Üniversitesi Eczacılık Fakültesi Dergisi Vol.47 Issue 3, pp.827-835

Belge Tipi:

Makale

Yayın Tarihi:

9/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001291230

pISSN:

1015-3918