Isolation and identification of Xanthomonas citri subsp. malvacearum,  cotton bacterial blight disease agent and determination of the  antibacterial activity of various plant essential oils

Soner Soylu; Merve Kara; Yusuf Gümüş; Emine Mine Soylu

Makale detayları

Isolation and identification of Xanthomonas citri subsp. malvacearum, cotton bacterial blight disease agent and determination of the antibacterial activity of various plant essential oils

Yazarlar:

Soner Soylu | Merve Kara | Yusuf Gümüş | Emine Mine Soylu

Yayıncı:

Harran University

Ana Başlıklar:

Fitopatoloji

Dergi:

Harran Tarım ve Gıda Bilimleri Dergisi Vol.28 Issue 2, pp.180-191

Belge Tipi:

Makale

Yayın Tarihi:

6/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001319585

pISSN:

2587-1358

Anahtar kelimeler:

Özet:

 Cotton is one of the most important industrial crops produced in large areas in Turkey. Cotton bacterial blight disease agent Xanthomonas citri subsp. malvacearum Syn. Xanthomonas axonopodis pv. malvacearum is a seed-borne pathogen. Plant essential oils are natural antimicrobial compounds that have the potential to be used as an alternative to chemical control of seed-borne phytopathogens. In this study, the bacterial pathogen X. citri subsp. malvacearum was isolated from cotton plants showing typical disease symptoms during the 2023 growing season in Diyarbakır provinces and the antibacterial effect of essential oils obtained from 11 different medicinal plants Thymus serpyllum, Origanum syriacum, Thymus syriacus, Origanum onites, Cistus laden, Salvia aramiensis, Laurus nobilis, Hypericum perforatum, Rosmarinus officinalis, Origanum majorana and Thymbra spicata were determined by disk diffusion method. Bacterial isolates, obtained from cotton plants showing typical disease symptoms, were identified as X. citri subsp. malvacearum by biochemical and pathogenicity tests and also MALDI-TOF analysis. The essential oils used in the study showed antibacterial activity against the bacterial agent by forming an inhibition zone with a diameter of 6.33-46.33 mm in the nutrient media. T. serpyllum and T. spicata 43.33 mm, O. syriacum 43.00 mm, T. syriacus 38.33 mm and O. onites 37.3 mm, were identified as the most effective essential oils respectively. The essential oils of H. perforatum 9.67 mm, L. nobilis 9.0 mm and S. aramiensis 6.33 mm showed relatively lower antibacterial activity against the disease agent. The results showed that essential oils of Thymus spp., Thymbra spp. and Origanum spp. have the potential to be used as an alternative to chemical control against seed-borne bacterial plant pathogens.

 Cotton is one of the most important industrial crops produced in large areas in Turkey. Cotton bacterial blight disease agent Xanthomonas citri subsp. malvacearum Syn. Xanthomonas axonopodis pv. malvacearum is a seed-borne pathogen. Plant essential oils are natural antimicrobial compounds that have the potential to be used as an alternative to chemical control of seed-borne phytopathogens. In this study, the bacterial pathogen X. citri subsp. malvacearum was isolated from cotton plants showing typical disease symptoms during the 2023 growing season in Diyarbakır provinces and the antibacterial effect of essential oils obtained from 11 different medicinal plants Thymus serpyllum, Origanum syriacum, Thymus syriacus, Origanum onites, Cistus laden, Salvia aramiensis, Laurus nobilis, Hypericum perforatum, Rosmarinus officinalis, Origanum majorana and Thymbra spicata were determined by disk diffusion method. Bacterial isolates, obtained from cotton plants showing typical disease symptoms, were identified as X. citri subsp. malvacearum by biochemical and pathogenicity tests and also MALDI-TOF analysis. The essential oils used in the study showed antibacterial activity against the bacterial agent by forming an inhibition zone with a diameter of 6.33-46.33 mm in the nutrient media. T. serpyllum and T. spicata 43.33 mm, O. syriacum 43.00 mm, T. syriacus 38.33 mm and O. onites 37.3 mm, were identified as the most effective essential oils respectively. The essential oils of H. perforatum 9.67 mm, L. nobilis 9.0 mm and S. aramiensis 6.33 mm showed relatively lower antibacterial activity against the disease agent. The results showed that essential oils of Thymus spp., Thymbra spp. and Origanum spp. have the potential to be used as an alternative to chemical control against seed-borne bacterial plant pathogens.

 Cotton is one of the most important industrial crops produced in large areas in Turkey. Cotton bacterial blight disease agent Xanthomonas citri subsp. malvacearum Syn. Xanthomonas axonopodis pv. malvacearum is a seed-borne pathogen. Plant essential oils are natural antimicrobial compounds that have the potential to be used as an alternative to chemical control of seed-borne phytopathogens. In this study, the bacterial pathogen X. citri subsp. malvacearum was isolated from cotton plants showing typical disease symptoms during the 2023 growing season in Diyarbakır provinces and the antibacterial effect of essential oils obtained from 11 different medicinal plants Thymus serpyllum, Origanum syriacum, Thymus syriacus, Origanum onites, Cistus laden, Salvia aramiensis, Laurus nobilis, Hypericum perforatum, Rosmarinus officinalis, Origanum majorana and Thymbra spicata were determined by disk diffusion method. Bacterial isolates, obtained from cotton plants showing typical disease symptoms, were identified as X. citri subsp. malvacearum by biochemical and pathogenicity tests and also MALDI-TOF analysis. The essential oils used in the study showed antibacterial activity against the bacterial agent by forming an inhibition zone with a diameter of 6.33-46.33 mm in the nutrient media. T. serpyllum and T. spicata 43.33 mm, O. syriacum 43.00 mm, T. syriacus 38.33 mm and O. onites 37.3 mm, were identified as the most effective essential oils respectively. The essential oils of H. perforatum 9.67 mm, L. nobilis 9.0 mm and S. aramiensis 6.33 mm showed relatively lower antibacterial activity against the disease agent. The results showed that essential oils of Thymus spp., Thymbra spp. and Origanum spp. have the potential to be used as an alternative to chemical control against seed-borne bacterial plant pathogens.

 Pamuk Türkiyede geniş alanlarda üretimi yapılan önemli endüstri bitkilerin başında gelir. Pamuk bakteriyel yanıklık hastalığı etmeni Xanthomonas citri subsp. malvacearum Syn. Xanthomonas axonopodis pv. malvacearum tohum kökenli bir patojendir. Bitki uçucu yağları tohum kökenli fitopatojen türlere karşı kimyasal mücadeleye alternatif olarak kullanım potansiyeline sahip doğal antimikrobiyal bileşiklerdir. Bu çalışmada, Diyarbakır ilinde 2023 yetiştirme sezonunda tipik hastalık belirtileri gösteren pamuk bitkilerinden X. citri subsp. malvacearum bakteriyel etmenin izolasyonu, tanılanması ve 11 farklı tıbbi bitkiden Thymus serpyllum, Origanum syriacum, Thymus syriacus, Origanum onites, Cistus laden, Salvia aramiensis, Laurus nobilis, Hypericum perforatum, Rosmarinus officinalis, Origanum majorana ve Thymbra spicata elde edilen uçucu yağların antibakteriyel etkisi disk difüzyon yöntemi ile belirlenmiştir. Tipik hastalık belirtileri gösteren pamuk bitkilerinden izole edilen izolatlar biyokimyasal ve patojenite testleri ve MALDI-TOF ile tür düzeyinde X. citri subsp. malvacearum olarak tanılanmıştır. Çalışmada kullanılan bitki uçucu yağları besi yerinde 6.33-46.33 mm çapında engelleme zonu oluşturmak suretiyle bakteriyel etmene karşı antibakteriyel etkinlik göstermiştir. En etkili uçucun yağlar sırasıyla T. serpyllum ve T. spicata 43.33 mm, O. syriacum 43.00 mm, T. syriacus 38.33 mm ve O. onites 37.3 mm olarak belirlenmiştir. H. perforatum 9.67 mm, L. nobilis 9.0 mm ve S. aramiensis 6.33 mm bitki uçucu yağları hastalık etmene karşı nispeten daha düşük oranlarda antibakteriyel etkinlik göstermiştir. Elde edilen sonuçlar Thymus spp., Thymbra spp. ve Origanum spp.ye ait bitki uçucu yağlarının tohum kökenli bitki patojeni bakteriyel hastalık etmenine karşı kimyasal mücadeleye alternatif mücadele yöntemi olarak uygulanma potansiyeline sahip olduğunu göstermiştir.

Makale detayları

Isolation and identification of Xanthomonas citri subsp. malvacearum, cotton bacterial blight disease agent and determination of the antibacterial activity of various plant essential oils

Yazarlar:

Soner Soylu | Merve Kara | Yusuf Gümüş | Emine Mine Soylu

Yayıncı:

Harran University

Ana Başlıklar:

Fitopatoloji

Dergi:

Harran Tarım ve Gıda Bilimleri Dergisi Vol.28 Issue 2, pp.180-191

Belge Tipi:

Makale

Yayın Tarihi:

6/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001319585

pISSN:

2587-1358

cotton

bacterial blight

Xanthomonas

essential oil

antibacterialpamuk

bakteriyel yanıklık

Xanthomonas

uçucu yağ

antibakteriyel

Sayı

Makale detayları

Yazarlar: Soner Soylu | Merve Kara | Yusuf Gümüş | Emine Mine Soylu

Yayıncı: Harran University

Ana Başlıklar: Fitopatoloji

Dergi: Harran Tarım ve Gıda Bilimleri Dergisi Vol.28 Issue 2, pp.180-191

Belge Tipi: Makale

Yayın Tarihi: 6/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001319585

pISSN: 2587-1358

Sayı

Yazarlar:

Soner Soylu | Merve Kara | Yusuf Gümüş | Emine Mine Soylu

Yayıncı:

Harran University

Ana Başlıklar:

Fitopatoloji

Dergi:

Harran Tarım ve Gıda Bilimleri Dergisi Vol.28 Issue 2, pp.180-191

Belge Tipi:

Makale

Yayın Tarihi:

6/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001319585

pISSN:

2587-1358