Simultaneous determination of dexpanthenol, lidocaine hydrochloride, and mepyramine maleate in combined pharmaceutical gel by capillary electrophoresis

Gülden Başmakçi AKYIL; Hayriye Eda Şatana KARA; Sezen Yarimkaya BAŞ; Nusret ERTAŞ; Nilgün GÜNDEN GÖĞER

doi:https://doi.org/10.3906/kim-1309-48

Makale detayları

Simultaneous determination of dexpanthenol, lidocaine hydrochloride, and mepyramine maleate in combined pharmaceutical gel by capillary electrophoresis

Yazarlar:

Gülden Başmakçi AKYIL | Hayriye Eda Şatana KARA | Sezen Yarimkaya BAŞ | Nusret ERTAŞ | Nilgün GÜNDEN GÖĞER

Yayıncı:

TUBITAK

Ana Başlıklar:

Kimya ve ilgili bilimler

Dergi:

Turkish Journal of Chemistry 2014 Vol.38 Issue 5, pp.756-764

Belge Tipi:

Makale

Yayın Tarihi:

4/2014

Belge Dili:

İngilizce

Document ID (Org No.):

2000727070

Sıklık:

İki Aylık

eISSN:

1303-6130

pISSN:

1300-0527

DOI:

https://doi.org/10.3906/kim-1309-48

Özet:

 

A new capillary electrophoresis method was developed for the simultaneous  determination of dexpanthenol (DEX), lidocaine (LID), and mepyramine (MEP)  in pharmaceutical preparations. The best results were obtained using 20 mM  (pH 3.0) phosphate buffer as the background electrolyte. Separation was  obtained using a fused-silica capillary (75 m m internal diameter, 50 cm  total length, 41 cm effective length) and a potential of +30 kV at 20  °C. Standards and samples were injected using a pressure injection  at 50 mbar for 5 s and the analytes were monitored at a detection wavelength  of 200 nm. Under optimum conditions, migration times were 2.457 min for MEP,  3.520 min for LID, and 7.363 min for DEX. The method was linear over the  ranges of 25--200 m g mL-1 for DEX, 7.5--60 m g mL-1 for  MEP, and 7.5--60 m g mL-1 for LID. Limit of detection (LOD) values  were 0.8 m g mL-1 for MEP, 1.8 m g mL-1 for LID, and 3.1  m g mL-1 for DEX. The developed method is accurate, precise,  sensitive, selective, and repeatable.

 

A new capillary electrophoresis method was developed for the simultaneous  determination of dexpanthenol (DEX), lidocaine (LID), and mepyramine (MEP)  in pharmaceutical preparations. The best results were obtained using 20 mM  (pH 3.0) phosphate buffer as the background electrolyte. Separation was  obtained using a fused-silica capillary (75 m m internal diameter, 50 cm  total length, 41 cm effective length) and a potential of +30 kV at 20  °C. Standards and samples were injected using a pressure injection  at 50 mbar for 5 s and the analytes were monitored at a detection wavelength  of 200 nm. Under optimum conditions, migration times were 2.457 min for MEP,  3.520 min for LID, and 7.363 min for DEX. The method was linear over the  ranges of 25--200 m g mL-1 for DEX, 7.5--60 m g mL-1 for  MEP, and 7.5--60 m g mL-1 for LID. Limit of detection (LOD) values  were 0.8 m g mL-1 for MEP, 1.8 m g mL-1 for LID, and 3.1  m g mL-1 for DEX. The developed method is accurate, precise,  sensitive, selective, and repeatable.

Makale detayları

Simultaneous determination of dexpanthenol, lidocaine hydrochloride, and mepyramine maleate in combined pharmaceutical gel by capillary electrophoresis

Yazarlar: Gülden Başmakçi AKYIL | Hayriye Eda Şatana KARA | Sezen Yarimkaya BAŞ | Nusret ERTAŞ | Nilgün GÜNDEN GÖĞER

Yayıncı: TUBITAK

Ana Başlıklar: Kimya ve ilgili bilimler

Dergi: Turkish Journal of Chemistry 2014 Vol.38 Issue 5, pp.756-764

Belge Tipi: Makale

Yayın Tarihi: 4/2014

Belge Dili: İngilizce

Document ID (Org No.): 2000727070

Sıklık: İki Aylık

eISSN: 1303-6130

pISSN: 1300-0527

DOI: https://doi.org/10.3906/kim-1309-48

Sayı

Yazarlar:

Gülden Başmakçi AKYIL | Hayriye Eda Şatana KARA | Sezen Yarimkaya BAŞ | Nusret ERTAŞ | Nilgün GÜNDEN GÖĞER

Yayıncı:

TUBITAK

Ana Başlıklar:

Kimya ve ilgili bilimler

Dergi:

Turkish Journal of Chemistry 2014 Vol.38 Issue 5, pp.756-764

Belge Tipi:

Makale

Yayın Tarihi:

4/2014

Belge Dili:

İngilizce

Document ID (Org No.):

2000727070

Sıklık:

İki Aylık

eISSN:

1303-6130

pISSN:

1300-0527

DOI:

https://doi.org/10.3906/kim-1309-48