Prevalence of Toxoplasma gondii in sheep meats purchased from retailstores in Central Anatolia, Turkey

KADER YILDIZ; FATMA ÇİĞDEM PİŞKİN; ARMAĞAN ERDEM ÜTÜK; SAMİ GÖKPINAR

Makale detayları

Prevalence of Toxoplasma gondii in sheep meats purchased from retailstores in Central Anatolia, Turkey

Yazarlar:

KADER YILDIZ | FATMA ÇİĞDEM PİŞKİN | ARMAĞAN ERDEM ÜTÜK | SAMİ GÖKPINAR

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2015 Vol.39 Issue 3, pp.328-332

Belge Tipi:

Makale

Yayın Tarihi:

8/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000765582

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

Anahtar kelimeler:

Özet:

 

Toxoplasmosis is one of the most important foodborne parasitic diseases of humans. In particular, sheep muscles are significant sources of infection in the transmission of toxoplasmosis. Carnivorism is the most important transmission route for human populations. The aim of this study was to detect the prevalence of Toxoplasma gondii tissue cysts in sheep meats in retail stories of Turkey. A total of 250 boneless sheep meat samples were purchased from randomly selected retail stores in different locations of Ankara and Kırıkkale provinces of Turkey. The homogenized meat samples were centrifuged with Percoll dilutions. The tissue cysts were removed by pipette and analyzed under light microscope. Additionally, nested PCR was used to detect T. Gondii DNA in the meat samples. Tissue cysts were observed in 21.2% of the meat samples with Percoll gradient centrifugation. The prevalences of the tissue cysts were detected as 20.8% in the meat samples obtained from Ankara and 22.4% from Kırıkkale (P > 0.05). T. Gondii DNA was detected in 40.8% of the meat samples with nested PCR.

 

Toxoplasmosis is one of the most important foodborne parasitic diseases of humans. In particular, sheep muscles are significant sources of infection in the transmission of toxoplasmosis. Carnivorism is the most important transmission route for human populations. The aim of this study was to detect the prevalence of Toxoplasma gondii tissue cysts in sheep meats in retail stories of Turkey. A total of 250 boneless sheep meat samples were purchased from randomly selected retail stores in different locations of Ankara and Kırıkkale provinces of Turkey. The homogenized meat samples were centrifuged with Percoll dilutions. The tissue cysts were removed by pipette and analyzed under light microscope. Additionally, nested PCR was used to detect T. Gondii DNA in the meat samples. Tissue cysts were observed in 21.2% of the meat samples with Percoll gradient centrifugation. The prevalences of the tissue cysts were detected as 20.8% in the meat samples obtained from Ankara and 22.4% from Kırıkkale (P > 0.05). T. Gondii DNA was detected in 40.8% of the meat samples with nested PCR.

Makale detayları

Prevalence of Toxoplasma gondii in sheep meats purchased from retailstores in Central Anatolia, Turkey

Yazarlar:

KADER YILDIZ | FATMA ÇİĞDEM PİŞKİN | ARMAĞAN ERDEM ÜTÜK | SAMİ GÖKPINAR

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2015 Vol.39 Issue 3, pp.328-332

Belge Tipi:

Makale

Yayın Tarihi:

8/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000765582

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

Toxoplasma gondii

Apicomplexa

sheep

meat

tissue cyst

Turkey

Sayı

Makale detayları

Prevalence of Toxoplasma gondii in sheep meats purchased from retailstores in Central Anatolia, Turkey

Yazarlar: KADER YILDIZ | FATMA ÇİĞDEM PİŞKİN | ARMAĞAN ERDEM ÜTÜK | SAMİ GÖKPINAR

Yayıncı: TUBITAK

Dergi: Turkish Journal of Veterinary and Animal Sciences 2015 Vol.39 Issue 3, pp.328-332

Belge Tipi: Makale

Yayın Tarihi: 8/2015

Belge Dili: Türkçe

Document ID (Org No.): 2000765582

Sıklık: İki Aylık

eISSN: 1303-6181

pISSN: 1300-0128

Toxoplasma gondii

Apicomplexa

sheep

meat

tissue cyst

Turkey

Sayı

Yazarlar:

KADER YILDIZ | FATMA ÇİĞDEM PİŞKİN | ARMAĞAN ERDEM ÜTÜK | SAMİ GÖKPINAR

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2015 Vol.39 Issue 3, pp.328-332

Belge Tipi:

Makale

Yayın Tarihi:

8/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000765582

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128