DNA Sequence Based Molecular Identification of Eustrongylides excisus Larvas (Nematoda) in Sander Lucioperca from Lake Eğirdir

Kevser Öztürk; Mehmet Oğuz Öztürk

Makale detayları

DNA Sequence Based Molecular Identification of Eustrongylides excisus Larvas (Nematoda) in Sander Lucioperca from Lake Eğirdir

Yazarlar:

Kevser Öztürk | Mehmet Oğuz Öztürk

Yayıncı:

Burdur Mehmet Akif Ersoy University

Ana Başlıklar:

Bitki Bilimi (Diğer)

Dergi:

Mehmet Akif Ersoy Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.14 Issue 2, pp.346-354

Belge Tipi:

Makale

Yayın Tarihi:

12/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001293215

pISSN:

1309-2243

Anahtar kelimeler:

Özet:

 In this study, 926-954 bp partially distinct nucleotide sequences which belong to the 18S and 28SrDNA gene loci from 3 Eustrongylides excisus samples were identified as identical and registered in GenBank OP480437-39. Nucleotide values in 18S and 28S rDNA gene sequences of Eustrongylides excisus samples were defined respectively: 26.94 27.26 A; 26.78-26.84 T; 28.30-28.62 G; 17.39-17.92 C. According to the sequence dataset distance matrix obtained by the Pairwise comparison method, there was a complete agreement 0 between the Eustrongylides excisus isolates identified in this study OP480437-39 and the Eustrongylides excisus isolate registered in the GenBank MK007967, MT415236, MK545494.The Eustrongylides excisus isolates were collected in the same cluster in Maximum likelihood filogram analysis. The internal transcribed spacer ITS region gene sequence results of the isolates confirmed the taxonomic position of Eustrongylides excisus, which was defined according to its anatomical and morphological features. More, 18S and 28S rDNA gene sequences were defined for the first time in this study on 3 samples of Eustrongylides excisus species in Lake Eğirdir, and contributions were made to the determination of genetic characteristics of the species.

 In this study, 926-954 bp partially distinct nucleotide sequences which belong to the 18S and 28SrDNA gene loci from 3 Eustrongylides excisus samples were identified as identical and registered in GenBank OP480437-39. Nucleotide values in 18S and 28S rDNA gene sequences of Eustrongylides excisus samples were defined respectively: 26.94 27.26 A; 26.78-26.84 T; 28.30-28.62 G; 17.39-17.92 C. According to the sequence dataset distance matrix obtained by the Pairwise comparison method, there was a complete agreement 0 between the Eustrongylides excisus isolates identified in this study OP480437-39 and the Eustrongylides excisus isolate registered in the GenBank MK007967, MT415236, MK545494.The Eustrongylides excisus isolates were collected in the same cluster in Maximum likelihood filogram analysis. The internal transcribed spacer ITS region gene sequence results of the isolates confirmed the taxonomic position of Eustrongylides excisus, which was defined according to its anatomical and morphological features. More, 18S and 28S rDNA gene sequences were defined for the first time in this study on 3 samples of Eustrongylides excisus species in Lake Eğirdir, and contributions were made to the determination of genetic characteristics of the species.

 In this study, 926-954 bp partially distinct nucleotide sequences which belong to the 18S and 28SrDNA gene loci from 3 Eustrongylides excisus samples were identified as identical and registered in GenBank OP480437-39. Nucleotide values in 18S and 28S rDNA gene sequences of Eustrongylides excisus samples were defined respectively: 26.94 27.26 A; 26.78-26.84 T; 28.30-28.62 G; 17.39-17.92 C. According to the sequence dataset distance matrix obtained by the Pairwise comparison method, there was a complete agreement 0 between the Eustrongylides excisus isolates identified in this study OP480437-39 and the Eustrongylides excisus isolate registered in the GenBank MK007967, MT415236, MK545494.The Eustrongylides excisus isolates were collected in the same cluster in Maximum likelihood filogram analysis. The internal transcribed spacer ITS region gene sequence results of the isolates confirmed the taxonomic position of Eustrongylides excisus, which was defined according to its anatomical and morphological features. More, 18S and 28S rDNA gene sequences were defined for the first time in this study on 3 samples of Eustrongylides excisus species in Lake Eğirdir, and contributions were made to the determination of genetic characteristics of the species.

 Bu çalışmada, 3Eustrongylides excisus örneğinden 18S ve 28SrDNA gen lokuslarına ait 926-954 bp kısmi belirgin nükleotid dizileri özdeş olarak tanımlanmış ve GenBankta kayıt altına alınmıştır OP480437-39. Eustrongylides excisus örneklerinin 18S ve 28SrDNA gen dizilerindeki nükleotid değerleri tanımlanmıştır:  26,94-27,26 A; 26,78-26,84 T;  28,30-28,62 G;  17,39-17,92 C. Pairwise comparison metodu ile elde edilen dizi veri kümesi mesafe matrisine göre, bu çalışmada tanımlanan Eustrongylides excisus izolatları OP480437-39 ile GenBankta kayıtlı Eustrongylides excisus izolatları MK007967, MT415236, MK545494 arasında tam bir uyum görülmüştür 0,0. Bu Eustrongylides excisus izolatları Maximum likelihood filogram analizinde aynı kümede toplanmıştır. İzolatların internal transkripsiyonlu ayırıcı ITS bölgesi gen sekansı sonuçları, Eustrongylides excisusun anatomik ve morfolojik özelliklerine göre tanımlanan taksonomik konumunu doğrulamıştır. Ayrıca, Eğirdir Gölündeki Eustrongylides excisus türüne ait 3 örnek üzerinden 18S ve 28SrDNA gen dizileri ilk defa bu çalışmada tanımlanmış ve bu türe ait genetik özelliklerin belirlenmesi çalışmalarına katkı yapılmıştır.

Makale detayları

DNA Sequence Based Molecular Identification of Eustrongylides excisus Larvas (Nematoda) in Sander Lucioperca from Lake Eğirdir

Yazarlar:

Kevser Öztürk | Mehmet Oğuz Öztürk

Yayıncı:

Burdur Mehmet Akif Ersoy University

Ana Başlıklar:

Bitki Bilimi (Diğer)

Dergi:

Mehmet Akif Ersoy Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.14 Issue 2, pp.346-354

Belge Tipi:

Makale

Yayın Tarihi:

12/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001293215

pISSN:

1309-2243

Eustrongylides excisus

DNA sequencing

Sander luciopercaEustrongylides excisus

DNA sekanslama

Sander lucioperca

Sayı

Makale detayları

DNA Sequence Based Molecular Identification of Eustrongylides excisus Larvas (Nematoda) in Sander Lucioperca from Lake Eğirdir

Yazarlar: Kevser Öztürk | Mehmet Oğuz Öztürk

Yayıncı: Burdur Mehmet Akif Ersoy University

Ana Başlıklar: Bitki Bilimi (Diğer)

Dergi: Mehmet Akif Ersoy Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.14 Issue 2, pp.346-354

Belge Tipi: Makale

Yayın Tarihi: 12/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001293215

pISSN: 1309-2243

Eustrongylides excisus

DNA sequencing

Sander luciopercaEustrongylides excisus

DNA sekanslama

Sander lucioperca

Sayı

Yazarlar:

Kevser Öztürk | Mehmet Oğuz Öztürk

Yayıncı:

Burdur Mehmet Akif Ersoy University

Ana Başlıklar:

Bitki Bilimi (Diğer)

Dergi:

Mehmet Akif Ersoy Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.14 Issue 2, pp.346-354

Belge Tipi:

Makale

Yayın Tarihi:

12/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001293215

pISSN:

1309-2243