Evaluation of the presence of SARS-CoV-2 in the aqueous humor and vitreous in patients undergoing combined phaco-vitrectomy surgery

Göksu Hande Naz Şimdivar; Emine Çiloğlu; Tuğba İncekalan; Neşe Çetin Doğan; Nevzat Ünal; Hivda Ülbegi Polat; Esin Akçael

Makale detayları

Evaluation of the presence of SARS-CoV-2 in the aqueous humor and vitreous in patients undergoing combined phaco-vitrectomy surgery

Yazarlar:

Göksu Hande Naz Şimdivar | Emine Çiloğlu | Tuğba İncekalan | Neşe Çetin Doğan | Nevzat Ünal | Hivda Ülbegi Polat | Esin Akçael

Yayıncı:

Pamukkale University

Ana Başlıklar:

Göz Hastalıkları

Dergi:

Pamukkale Tıp Dergisi Vol.16 Issue 3, pp.376-382

Belge Tipi:

Makale

Yayın Tarihi:

1/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001282764

pISSN:

1308-0865

Anahtar kelimeler:

Özet:

 Purpose: To investigate the presence of virus in the aqueous humor and vitreous of patients undergoing elective combined cataract and pars plana vitrectomy during the Coronavirus disease 2019 COVID-19 pandemic. Materials and methods: In this prospective cross-sectional study, of the patients to undergo elective surgery, those who had a negative severe acute respiratory syndrome coronavirus 2  SARS-CoV-2 nasal swab test 24-72 hours prior to the surgery and who were asymptomatic were included. SARS-CoV-2 IgG antibodies were evaluated in blood samples taken 24-72 hours before the operation. 0.1 cc of aqueous humor and 0.1 cc of vitreous fluid were aspirated at the beginning of the surgery. The presence of SARS-CoV-2 viral ribonucleic acid RNA was evaluated by real-time reverse transcriptase polymerase chain reaction RT-PCR. Results: Of 66 participants, 39 were male 59.1 while 27 were female 40.9. Twenty-five patients 37.8 had a history of COVID-19 between 20 days-60 days mean 49 days before the surgery. There were 58 patients 87.9 with a history of vaccination before the operation and 8 patients 12.1 without a history of vaccination. No SARS CoV 2 RNA was detected in the aqueous humor and vitreous samples of any patient. IgG against SARSCoV-2 was detected in 3 patients who had not been vaccinated against COVID-19 before and had no known history of COVID-19. Conclusion: We did not find any SARS-CoV-2 viral genetic material in the aqueous and vitreous fluids of asymptomatic participants whose nasal swab test results were negative, even if they recently had COVID-19. There is a need for more comprehensive studies investigating the latency of the virus in immune-privileged areas such as the eye, how long it remains in the eye even if it is withdrawn from the circulation, and possible eye diseases that it may cause during the convalescence period.

 Purpose: To investigate the presence of virus in the aqueous humor and vitreous of patients undergoing elective combined cataract and pars plana vitrectomy during the Coronavirus disease 2019 COVID-19 pandemic. Materials and methods: In this prospective cross-sectional study, of the patients to undergo elective surgery, those who had a negative severe acute respiratory syndrome coronavirus 2  SARS-CoV-2 nasal swab test 24-72 hours prior to the surgery and who were asymptomatic were included. SARS-CoV-2 IgG antibodies were evaluated in blood samples taken 24-72 hours before the operation. 0.1 cc of aqueous humor and 0.1 cc of vitreous fluid were aspirated at the beginning of the surgery. The presence of SARS-CoV-2 viral ribonucleic acid RNA was evaluated by real-time reverse transcriptase polymerase chain reaction RT-PCR. Results: Of 66 participants, 39 were male 59.1 while 27 were female 40.9. Twenty-five patients 37.8 had a history of COVID-19 between 20 days-60 days mean 49 days before the surgery. There were 58 patients 87.9 with a history of vaccination before the operation and 8 patients 12.1 without a history of vaccination. No SARS CoV 2 RNA was detected in the aqueous humor and vitreous samples of any patient. IgG against SARSCoV-2 was detected in 3 patients who had not been vaccinated against COVID-19 before and had no known history of COVID-19. Conclusion: We did not find any SARS-CoV-2 viral genetic material in the aqueous and vitreous fluids of asymptomatic participants whose nasal swab test results were negative, even if they recently had COVID-19. There is a need for more comprehensive studies investigating the latency of the virus in immune-privileged areas such as the eye, how long it remains in the eye even if it is withdrawn from the circulation, and possible eye diseases that it may cause during the convalescence period.

 Purpose: To investigate the presence of virus in the aqueous humor and vitreous of patients undergoing elective combined cataract and pars plana vitrectomy during the Coronavirus disease 2019 COVID-19 pandemic. Materials and methods: In this prospective cross-sectional study, of the patients to undergo elective surgery, those who had a negative severe acute respiratory syndrome coronavirus 2  SARS-CoV-2 nasal swab test 24-72 hours prior to the surgery and who were asymptomatic were included. SARS-CoV-2 IgG antibodies were evaluated in blood samples taken 24-72 hours before the operation. 0.1 cc of aqueous humor and 0.1 cc of vitreous fluid were aspirated at the beginning of the surgery. The presence of SARS-CoV-2 viral ribonucleic acid RNA was evaluated by real-time reverse transcriptase polymerase chain reaction RT-PCR. Results: Of 66 participants, 39 were male 59.1 while 27 were female 40.9. Twenty-five patients 37.8 had a history of COVID-19 between 20 days-60 days mean 49 days before the surgery. There were 58 patients 87.9 with a history of vaccination before the operation and 8 patients 12.1 without a history of vaccination. No SARS CoV 2 RNA was detected in the aqueous humor and vitreous samples of any patient. IgG against SARSCoV-2 was detected in 3 patients who had not been vaccinated against COVID-19 before and had no known history of COVID-19. Conclusion: We did not find any SARS-CoV-2 viral genetic material in the aqueous and vitreous fluids of asymptomatic participants whose nasal swab test results were negative, even if they recently had COVID-19. There is a need for more comprehensive studies investigating the latency of the virus in immune-privileged areas such as the eye, how long it remains in the eye even if it is withdrawn from the circulation, and possible eye diseases that it may cause during the convalescence period.

 Amaç: Coronavirus 2019 COVID-19 pandemisi döneminde elektif kombine katarakt ve pars plana vitrektomi cerrahisi uygulanan hastaların aköz ve vitreus sıvısında virus varlığının araştırılması amaçlandı. Gereç ve yöntem: Bu prospektif kesitsel çalışmaya elektif kombine katarakt ve pars plana vitrektomi ameliyatı yapılacak hastalardan preoperatif 24-72 saat önceki SARS-CoV-2 nazal sürüntü testi negatif çıkan, COVID-19 bakımından asemptomatik olanlar dahil edildi. Preoperatif 24-72 saat önce alınan kan numunelerinde SARS-CoV-2 IgG antikoru değerlendirildi. Katarakt cerrahisinin başlangıcında 0.1 cc aköz hümör, vitrektominin başlangıcında ise 0.1 cc vitreus sıvısı aspire edildi. SARS-CoV-2 viral ribonucleic acid RNA varlığı real-time reverse transcriptase polymerase chain reaction RT-PCR ile değerlendirildi. Bulgular: Otuz dokuzu erkek 59,1, 27si kadın 40,9 olmak üzere 66 katılımcının göz içi sıvı örnekleri başarıyla analiz edildi. 25 hastada 37,8 operasyondan 20 gün-60 gün önce ortalama 49 gün COVID-19 geçirme öyküsü mevcuttu. Operasyondan önce aşı öyküsü bulunan 58 hasta 87,9, aşı öyküsü bulunmayan 8 hasta 12,1 mevcut idi. Hiçbir hastanın aköz hümör ve vitreus örneklerinde SARSCoV2 RNAsına rastlanmadı. Daha önce COVID-19 aşısı yapılmamış ve bilinen COVID-19 geçirme öyküsü olmayan 3 hastada SARSCoV-2ye karşı gelişen IgG tespit edildi. Sonuç: Bu çalışmada COVID-19 bakımından asemptomatik, nazal sürüntü testi negatif katılımcıların aköz ve vitreus sıvılarında SARS-CoV-2 viral genetik materyaline rastlamadık. Virüsün göz gibi immün ayrıcalıklı alanlarda latent kalma durumunu, dolaşımdan çekilse bile gözde ne kadar süre kaldığını ve nekahat döneminde neden olabileceği olası göz hastalıklarını araştıran daha kapsamlı çalışmalara ihtiyaç vardır.

Makale detayları

Evaluation of the presence of SARS-CoV-2 in the aqueous humor and vitreous in patients undergoing combined phaco-vitrectomy surgery

Yazarlar:

Göksu Hande Naz Şimdivar | Emine Çiloğlu | Tuğba İncekalan | Neşe Çetin Doğan | Nevzat Ünal | Hivda Ülbegi Polat | Esin Akçael

Yayıncı:

Pamukkale University

Ana Başlıklar:

Göz Hastalıkları

Dergi:

Pamukkale Tıp Dergisi Vol.16 Issue 3, pp.376-382

Belge Tipi:

Makale

Yayın Tarihi:

1/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001282764

pISSN:

1308-0865

Aqueous humor

COVID-19

SARS-CoV-2

vitreous

Aköz hümor

COVID-19

SARS-CoV-2

vitreous

Sayı

Makale detayları

Yazarlar: Göksu Hande Naz Şimdivar | Emine Çiloğlu | Tuğba İncekalan | Neşe Çetin Doğan | Nevzat Ünal | Hivda Ülbegi Polat | Esin Akçael

Yayıncı: Pamukkale University

Ana Başlıklar: Göz Hastalıkları

Dergi: Pamukkale Tıp Dergisi Vol.16 Issue 3, pp.376-382

Belge Tipi: Makale

Yayın Tarihi: 1/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001282764

pISSN: 1308-0865

Sayı

Yazarlar:

Göksu Hande Naz Şimdivar | Emine Çiloğlu | Tuğba İncekalan | Neşe Çetin Doğan | Nevzat Ünal | Hivda Ülbegi Polat | Esin Akçael

Yayıncı:

Pamukkale University

Ana Başlıklar:

Göz Hastalıkları

Dergi:

Pamukkale Tıp Dergisi Vol.16 Issue 3, pp.376-382

Belge Tipi:

Makale

Yayın Tarihi:

1/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001282764

pISSN:

1308-0865