Micropropagation of Pear Clone Rootstocks OHxF 97 and OHxF 333

Yunus Emre Tuncel; Bekir Şan

Makale detayları

Micropropagation of Pear Clone Rootstocks OHxF 97 and OHxF 333

Yazarlar:

Yunus Emre Tuncel | Bekir Şan

Yayıncı:

Ondokuz Mayıs University

Dergi:

Anadolu Tarım Bilimleri Dergisi Vol.38 Issue 2, pp.315-330

Belge Tipi:

Makale

Yayın Tarihi:

4/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001291419

pISSN:

1308-8750

Anahtar kelimeler:

Özet:

 In this study, the effects of growth regulator combinations on micropropagation of OHxF 97 and OHxF 333 pear clone rootstocks were investigated. In the proliferation phase of the research, the effects of different doses of BAP, a growth regulator, sucrose and glucose as a carbon source, and silver thiosulfate were investigated. In the first trial of the proliferation stage, it was determined that the addition of 2 mgl BAP  0.5 mgl IBA to MS nutrient medium gave the highest shoot number per explant 5.2 and 2.1 unitsexplant, respectively in both OHxF 97 and OHxF 333 rootstocks. In the second experiment of proliferation, the effects of carbon source and silver thiosulfate on shoot number per explant and shoot length in OHxF 97 rootstock were found to be insignificant. It was determined that the addition of glucose and glucose  5 µM silver thiosulfate to the nutrient medium in OHxF 333 rootstock significantly increased the number of shoots per explant 2.6 and 2.5, respectively compared to the control. In the rooting stage, the effect of combinations of IBA and activated charcoal was determined in the first trial. In the study, the highest rooting rate occurred in ½ MS medium containing 2 mgl IBA without activated charcoal on both rootstocks. It was determined that the addition of activated charcoal to the nutrient medium decreased the rooting rate compared to the control. In the second experiment, the effect of 2 mgl IBA  100 or 150 mgl organic iron Fe-EDDHA application was investigated. In the second stage of the rooting study, the highest rooting rate was achieved in ½ MS medium with 2 mgl IBA  150 mgl iron added in both rootstocks. It was determined that the addition of organic iron to the nutrient medium increased the rooting rate.

 In this study, the effects of growth regulator combinations on micropropagation of OHxF 97 and OHxF 333 pear clone rootstocks were investigated. In the proliferation phase of the research, the effects of different doses of BAP, a growth regulator, sucrose and glucose as a carbon source, and silver thiosulfate were investigated. In the first trial of the proliferation stage, it was determined that the addition of 2 mgl BAP  0.5 mgl IBA to MS nutrient medium gave the highest shoot number per explant 5.2 and 2.1 unitsexplant, respectively in both OHxF 97 and OHxF 333 rootstocks. In the second experiment of proliferation, the effects of carbon source and silver thiosulfate on shoot number per explant and shoot length in OHxF 97 rootstock were found to be insignificant. It was determined that the addition of glucose and glucose  5 µM silver thiosulfate to the nutrient medium in OHxF 333 rootstock significantly increased the number of shoots per explant 2.6 and 2.5, respectively compared to the control. In the rooting stage, the effect of combinations of IBA and activated charcoal was determined in the first trial. In the study, the highest rooting rate occurred in ½ MS medium containing 2 mgl IBA without activated charcoal on both rootstocks. It was determined that the addition of activated charcoal to the nutrient medium decreased the rooting rate compared to the control. In the second experiment, the effect of 2 mgl IBA  100 or 150 mgl organic iron Fe-EDDHA application was investigated. In the second stage of the rooting study, the highest rooting rate was achieved in ½ MS medium with 2 mgl IBA  150 mgl iron added in both rootstocks. It was determined that the addition of organic iron to the nutrient medium increased the rooting rate.

 In this study, the effects of growth regulator combinations on micropropagation of OHxF 97 and OHxF 333 pear clone rootstocks were investigated. In the proliferation phase of the research, the effects of different doses of BAP, a growth regulator, sucrose and glucose as a carbon source, and silver thiosulfate were investigated. In the first trial of the proliferation stage, it was determined that the addition of 2 mgl BAP  0.5 mgl IBA to MS nutrient medium gave the highest shoot number per explant 5.2 and 2.1 unitsexplant, respectively in both OHxF 97 and OHxF 333 rootstocks. In the second experiment of proliferation, the effects of carbon source and silver thiosulfate on shoot number per explant and shoot length in OHxF 97 rootstock were found to be insignificant. It was determined that the addition of glucose and glucose  5 µM silver thiosulfate to the nutrient medium in OHxF 333 rootstock significantly increased the number of shoots per explant 2.6 and 2.5, respectively compared to the control. In the rooting stage, the effect of combinations of IBA and activated charcoal was determined in the first trial. In the study, the highest rooting rate occurred in ½ MS medium containing 2 mgl IBA without activated charcoal on both rootstocks. It was determined that the addition of activated charcoal to the nutrient medium decreased the rooting rate compared to the control. In the second experiment, the effect of 2 mgl IBA  100 or 150 mgl organic iron Fe-EDDHA application was investigated. In the second stage of the rooting study, the highest rooting rate was achieved in ½ MS medium with 2 mgl IBA  150 mgl iron added in both rootstocks. It was determined that the addition of organic iron to the nutrient medium increased the rooting rate.

 Bu çalışmada, OHxF-97 ve OHxF-333 armut Pyrus spp. klon anaçlarında büyüme düzenleyici madde kombinasyonlarının mikroçoğaltım üzerine etkileri araştırılmıştır. Araştırmanın çoğaltma aşamasında büyümeyi düzenleyici maddelerden BAPın farklı dozları, karbon kaynağı olarak sakkaroz ve glikoz ile gümüş tiyosülfatın etkileri araştırılmıştır. Çoğaltma aşamasının ilk denemesinde hem OHxF-97 hem OHxF-333 anaçlarında MS besin ortamına 2 mgl BAP0.5 mgl IBA ilavesinin en yüksek eksplant başına sürgün sayısını sırasıyla, 5.2 ve 2.1 adeteksplant verdiği belirlenmiştir. Çoğaltmanın 2. denemesinde ise OHxF-97 anacında karbon kaynağı ve gümüş tiyosülfatın eksplant başına sürgün sayısı ve sürgün uzunluğu üzerine etkileri önemsiz bulunmuştur. OHxF-333 anacında ise besin ortamına glikoz ve glikoz5 µM gümüş tiyosülfat ilavesinin kontrole göre eksplant başına sürgün sayısını sırasıyla, 2.6 ve 2.5 adeteksplant önemli oranda artırdığı belirlenmiştir. Köklendirme aşamasının ilk denemesinde, IBA ve aktif kömür kombinasyonlarının etkisi belirlenmiştir. Buna göre en yüksek köklenme oranı her iki anaçta da 2 mgl IBA içeren aktif kömürsüz ½ MS ortamında meydana gelmiştir. Besin ortamına aktif kömür ilave edilmesinin köklenme oranını kontrole göre azalttığı saptanmıştır. Köklendirmenin ikinci denemesinde ise 2 mgl IBA  100 ya da 150 mgl organik demir Fe-EDDHA uygulamasının etkisi incelenmiş olup en yüksek köklenme oranı her iki anaçta da 2 mgl IBA150 mgl demir ilave edilniş ½ MS ortamında gerçekleşmiştir. Sonuçta, besin ortamına organik demir ilavesinin köklenme oranını arttırdığı tespit edilmiştir.

Makale detayları

Micropropagation of Pear Clone Rootstocks OHxF 97 and OHxF 333

Yazarlar:

Yunus Emre Tuncel | Bekir Şan

Yayıncı:

Ondokuz Mayıs University

Dergi:

Anadolu Tarım Bilimleri Dergisi Vol.38 Issue 2, pp.315-330

Belge Tipi:

Makale

Yayın Tarihi:

4/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001291419

pISSN:

1308-8750

Aktif kömür

Armut

Demir

Gümüş tiyosülfatAktif kömür

Armut

Demir

Gümüş tiyosülfat

Sayı

Makale detayları

Yazarlar: Yunus Emre Tuncel | Bekir Şan

Yayıncı: Ondokuz Mayıs University

Dergi: Anadolu Tarım Bilimleri Dergisi Vol.38 Issue 2, pp.315-330

Belge Tipi: Makale

Yayın Tarihi: 4/2023

Belge Dili: Türkçe

Document ID (Org No.): 2001291419

pISSN: 1308-8750

Sayı

Yazarlar:

Yunus Emre Tuncel | Bekir Şan

Yayıncı:

Ondokuz Mayıs University

Dergi:

Anadolu Tarım Bilimleri Dergisi Vol.38 Issue 2, pp.315-330

Belge Tipi:

Makale

Yayın Tarihi:

4/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001291419

pISSN:

1308-8750