The Effects of Dexamethasone on Claudin Tight Junction proteins in 18-day Embryonic Chick Intestine

Özkan ÖZDEN

Makale detayları

The Effects of Dexamethasone on Claudin Tight Junction proteins in 18-day Embryonic Chick Intestine

Yazarlar:

Özkan ÖZDEN

Yayıncı:

Erciyes University

Dergi:

Journal of The Faculty of Veterinary Medicine Erciyes University 2018 Vol.15 Issue 1, pp.6-10

Belge Tipi:

Makale

Yayın Tarihi:

4/2018

Belge Dili:

İngilizce

Document ID (Org No.):

2000413302

eISSN:

2667-5498

pISSN:

1304-7280

Özet:

 


Claudin (cldn) protein family is the main component
of tight junction (TJ) strands and it is directly involved in the barrier function
of TJs. In addition to the contributing to the barrier function of TJs in
mature tissues, these junctions are also present and required for normal
embryonic development of epithelial sheets. Glucocorticoids (GC) are essential
for the maturation of numerous tissues including the intestine during embryonic
development. They also have stimulatory effects on the integrity of tight
junctions. In this study, the effect of a synthetic glucocorticoid,
dexamethasone (DEX) on mRNA expression of tight junctional proteins was
investigated in 18-day embryonic chick intestine in vitro using quantitative
Real-Time PCR (RT-PCR) analyses. Pre-hatched chick intestine will provide a
convenient model because the organization of the barrier is not completely
established, and it is relatively easy to maintain its morphological integrity
for at least 24 hours in an organ culture system. RT-PCR analyses revealed that
both claudin-3 (cldn-3) and claudin-5 (cldn-5) tight junction gene expressions
were induced in response to DEX in a dose dependent manner. 

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Makale detayları

The Effects of Dexamethasone on Claudin Tight Junction proteins in 18-day Embryonic Chick Intestine

Yazarlar: Özkan ÖZDEN

Yayıncı: Erciyes University

Dergi: Journal of The Faculty of Veterinary Medicine Erciyes University 2018 Vol.15 Issue 1, pp.6-10

Belge Tipi: Makale

Yayın Tarihi: 4/2018

Belge Dili: İngilizce

Document ID (Org No.): 2000413302

eISSN: 2667-5498

pISSN: 1304-7280

Sayı

Yazarlar:

Özkan ÖZDEN

Yayıncı:

Erciyes University

Dergi:

Journal of The Faculty of Veterinary Medicine Erciyes University 2018 Vol.15 Issue 1, pp.6-10

Belge Tipi:

Makale

Yayın Tarihi:

4/2018

Belge Dili:

İngilizce

Document ID (Org No.):

2000413302

eISSN:

2667-5498

pISSN:

1304-7280